13 results about "Tetrahydrofolates" patented technology

The invention relates to the technical field of medicines, in particular to a preparation method of calcium L-5-methyltetrahydrofolate. According to the method, N-(4-aminobenzoyl)-L-glutamic acid, 2, 4, 5-triamino-6-hydroxypyrimidine sulfate and propane trihalide are adopted as starting raw materials, and tetrahydrofolic acid is synthesized through a one-pot method; compared with synthesis of L-5-methyl calcium tetrahydrofolate by using high-purity folic acid as an initial raw material, the process route does not involve use of chemical reagents such as sodium borohydride, zinc powder and some noble metal catalysts any more, the reaction is easier to control, the cost is lower, the safety is higher, the method is more environmentally friendly, and industrial production is easier to carry out.

The invention discloses a 5-methyltetrahydrofolic acid detection method, and belongs to the technical field of detection. According to the invention, a dual-mode aptamer sensor for 5-MTHF is constructed. According to the prepared aptamer sensor, through the synergistic effect of nanometer material catalysis, DNA walking amplification and CRISPR-Cas12a cis-cleavage, ultra-sensitive quantitative detection of 5-MTHF is achieved, and the detection limit is as low as 0.044 ng / mL (electrochemistry) and 0.114 ng / mL (colorimetric method). In addition, good performance is also shown in a marked real sample, and t test analysis results (pgt; 0.05) shows that there is no significant difference, which shows that the dual-mode detection has excellent stability and reliability.

Compounds comprising a radical of a therapeutic agent conjugated to a radical of raltitrexed, 5-methyltetrahydrofolate (5-MTHF), an analog of raltitrexed, or an analog of 5-MTHF via a linker; compositions comprising same; and a method of immunomodulating Tregs.

The invention relates to a method for simultaneously detecting multiple folic acid substances, and relates to the technical field of analysis and detection, and the method comprises the following steps: S1, preparing a to-be-detected sample solution; s2, quantitatively detecting the folic acid substances in the to-be-detected sample solution by adopting an ultra-high performance liquid chromatography-mass spectrometry method; wherein the chromatographic separation conditions of the ultra-high performance liquid chromatography-mass spectrometry method are as follows: the chromatographic column is Phenomenex LunaOmega Polar C18, the column temperature is 20-30 DEG C, and the column temperature is 20-30 DEG C; mobile phases are as follows: a phase A is ultrapure water containing acetic acid and VC, a phase B is methanol containing acetic acid and VC, and gradient elution is performed. According to the application, the synchronous detection of five target objects (folic acid, tetrahydrofolic acid, 5-methyltetrahydrofolic acid, 5-formyltetrahydrofolic acid and 10-formyltetrahydrofolic acid) can be completed in a short time, the sensitivity is very high, and a reliable analysis tool is provided for functional food development and nutrition evaluation.

The present invention relates to formulations of folate, in particular D-glucosamine 5-methyl-(6S)-tetrahydrofolate, in the form of a suspension in a mixture of medium chain triglyceride oil and stearin. Medium chain triglyceride oil and stearin having a water content of less than 0.5 wt% and a free glycerol and / or ethylene glycol content of less than 2 wt% are preferably used. The invention also discloses a method for preparing the preparation.

The invention provides a kit for detecting homocysteine and metabolites thereof in serum. The kit comprises a calibrator and a sample releasing agent, the calibrator comprises a component 1 and a component 2; the component 1 comprises a BSA (Bovine Serum Albumin) matrix and a first protective agent; the component 2 comprises homocysteine, metabolite of the homocysteine and a second protective agent; each of the first protective agent and the second protective agent is prepared from ascorbic acid, dithiothreitol, N-acetyl-DL-penicillamine and EDTA (Ethylene Diamine Tetraacetic Acid). According to the present invention, the 5-methyltetrahydrofolic acid and the homocysteine which are easily oxidized are well protected, and the synchronous detection of the folic acid, the 5-methyltetrahydrofolic acid, the vitamin B2, the pyridoxal phosphate, the methylmalonic acid, the homocysteine and the methionine is achieved. The kit has good stability at 4 DEG C and can meet the use requirement.

The application discloses 6S-5-methyltetrahydrofolate calcium fortified sterilized milk and a preparation method thereof, and steps are as follows: S1, acceptance of raw milk; S2, cleaning of milk; S3, storage of milk; S4, standardization; S5, preheating; S6, primary homogenization; S7, pasteurization; S8, secondary homogenization; S9, cooling; after homogenization, the sterilized milk is rapidly cooled to 4 DEG C; S10, aseptic filling; filling is performed at about 200mL per bag, temperature control is below 20 DEG C, and finished products enter a 4 DEG C cold storage. The preparation method of the (6S)-5-MTHF-Ca fortified sterilized milk can combine (6S)-5-MTHF-Ca and sterilized milk well by researching optimal preparation conditions, improves the stability of (6S)-5-MTHF-Ca in the sterilized milk, responds to the expansion of the application field of (6S)-5-MTHF-Ca, and provides a new way for pregnant women and lactating women to supplement folic acid.

The invention provides a pharmaceutical composition. The pharmaceutical composition is prepared from an oral anticoagulant drug, folic acid, 5-methyltetrahydrofolic acid and pharmaceutically acceptable auxiliary materials. The invention also provides application of the pharmaceutical composition in preparation of drugs for treating anti-phospholipid syndromes. The pharmaceutical composition has the advantages that the pharmaceutical composition is particularly suitable for patients with repeated thrombus formation, and the clinical benefit of the pharmaceutical composition is remarkably superior to that of single-use anti-oral coagulation drugs. In addition, a patient can take the medicine conveniently, and the medical cost is reduced.

The invention provides a pharmaceutical composition. The pharmaceutical composition is prepared from statins lipid-lowering drugs, folic acid, 5-methyltetrahydrofolic acid and pharmaceutically acceptable auxiliary materials. The invention also provides application of the pharmaceutical composition in preparation of medicines for preventing gallstone. The pharmaceutical composition disclosed by the invention has the advantages that the pharmaceutical composition is particularly suitable for preventing gallbladder cholesterol calculus, and the clinical benefit is obviously superior to that of a single statin lipid-lowering drug. In addition, a patient can take the medicine conveniently, and the medical cost is reduced.

The invention discloses a DNA (deoxyribonucleic acid) methylation anti-aging composition for improving sleep and skin states and resisting aging. Each capsule comprises the following substances in parts by weight: 96.00 + / -6 mg of an HPMC hollow capsule, 305.00 mg of alpha-ketoglutarate calcium (98%), 10.00 mg of pyrroloquinoline quinone disodium salt (98%), 10.00 mg of spermidine trihydrochloride, 0.60 mg of L-5-methyl calcium tetrahydrofolate (95%-102%), 2.9 mg of riboflavin, 3.5 mg of pyridoxine hydrochloride, and 98.00 mg of microcrystalline cellulose, silicon dioxide and magnesium stearate. The composition can stimulate cell viability, promote cell viability recovery and morphological repair, beautify skin, reduce inflammation, resist oxidation, help sleep and resist aging, and Ca-AKG, PQQ, spermidine, cofactor folic acid and other B vitamins are scientifically proportioned to synergistically activate cell metabolism, autophagy and mitochondrial functions. Long-term supplementation of the formula can improve sleep quality and improve deep sleep time and sleep efficiency; meanwhile, collagen generation is promoted, skin elasticity and glossiness are enhanced, and wrinkles and color spots are reduced.

The utility model relates to the technical field of mixing tanks, discloses a high-speed shearing vacuum mixing tank for calcium 6S-5-methyltetrahydrofolate, and solves the problem that the calcium 6S-5-methyltetrahydrofolate is easy to degrade or lose biological activity due to temperature rise of mixed materials in the mixing process. A high-speed shearing vacuum mixing tank for calcium 6S-5-methyltetrahydrofolate comprises a tank body, a tank cover, an annular supporting frame, supporting legs and a mixing assembly, and the mixing assembly comprises the tank cover, a motor, a rotating shaft, a driving gear, a driven gear, a first cooling pipe, a second cooling pipe, a mounting base and stirring blades. The first cooling pipe, the second cooling pipe and the stirring blade are driven by the motor to rotate, the second cooling pipe and the stirring blade rotate to mix materials in the tank body, when the mixing temperature is high, a cooling medium is injected from the first cooling pipe, the first cooling pipe and the second cooling pipe form a cooling loop, and the cooling efficiency is improved. The condition that the biological activity of the calcium 6S-5-methyltetrahydrofolate is lost due to too high temperature is prevented.

The present application provides a yeast strain capable of producing 5-methyltetrahydrofolate, a construction method and a fermentation method thereof, and the use of the strain or its fermentation product in the preparation of 5-methyltetrahydrofolate or its salt, in the supplementation of 5-methyltetrahydrofolate or its salt in a subject. Further, the present application also provides the use of the strain or its fermentation product in the prevention and / or treatment of Alzheimer's disease.