Phosphoglyceric acid mutase gene segment capable of improving yield of paddy rice and application of phosphoglyceric acid mutase gene segment

A technology of rice and fragments, applied in the fields of application, genetic engineering, plant genetic improvement, etc., to achieve the effect of high accuracy and simple operation

Active Publication Date: 2018-07-06
INST OF BOTANY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current breeding method is still mainly regional, and the ability to find gene types and their exact sequences that can increase yield in multiple regions is still a limiting factor for molecular breeding

Method used

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  • Phosphoglyceric acid mutase gene segment capable of improving yield of paddy rice and application of phosphoglyceric acid mutase gene segment
  • Phosphoglyceric acid mutase gene segment capable of improving yield of paddy rice and application of phosphoglyceric acid mutase gene segment
  • Phosphoglyceric acid mutase gene segment capable of improving yield of paddy rice and application of phosphoglyceric acid mutase gene segment

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Embodiment 1, design and synthesis of primers

[0068] Phosphoside oleic acid is one of the basic raw materials for the synthesis of various substances in rice. In the rice genome, one gene (LOC_Os01g60190.2) on chromosome 1 was annotated as a gene encoding phosphoside oleate mutase (EC 5.4.2.12, 2,3-bisphosphoglycerate-independent phosphoglycerate mutase 1; OsIPGAM1) (hereinafter abbreviated as OsIPGAM1 gene). Through a large number of preliminary experiments and sequence comparisons, the inventors of the present invention have found that there are two allele fragments in the OsIPGAM1 gene (one kind of allele fragment is named as the allele fragment OsIPGAM1_a as shown in sequence 1 of the sequence table; the other An allele fragment is shown in Sequence 2 of the Sequence Listing, named allele fragment OsIPGAM1_b), and they are related to rice yield per plant.

[0069] A specific primer pair was designed according to the above two allelic fragments, consisting of pri...

Embodiment 2

[0072] Example 2, Establishment of a typing method based on allele fragments in rice

[0073] The established method is as follows:

[0074] 1. Using the genomic DNA (about 10-100 ng) of the rice to be tested as a template, a specific primer pair composed of primer 1 and primer 2 is used to perform PCR amplification to obtain a PCR amplification product.

[0075] The reaction program of PCR amplification: 95°C for 5 minutes; 35 cycles of 95°C for 30 seconds, 60°C for 1 minute, and 72°C for 1 minute; 72°C for 8 minutes.

[0076] 2. After step 1 is completed, the PCR amplification product is sequenced, and the following judgment is made according to the sequencing result: if there is only one PCR amplification product, and as shown in sequence 1 in the sequence table, the genotype of the rice to be tested is OsIPGAM1_a Homozygous; if there is only one PCR amplification product, and as shown in sequence 2 in the sequence listing, the genotype of the rice to be tested is OsIPGAM1...

Embodiment 3

[0078] Example 3, Correlation analysis between genotypes based on allelic fragments and rice yield per plant in rice

[0079] 1. Statistical yield per plant of different rice varieties

[0080] In 2014, multiple rice varieties were planted in Sanya City and Beijing City, Hainan Province (see Table 1 and Table 2 for details, see the second column for the name of the rice variety, see the third column for the origin of the rice variety, and see the 2009 number of some rice varieties in column 6). A completely randomized block test design was adopted in the field. After the rice is mature, the rice plants are harvested per plant, weighed, and averaged to obtain the yield per plant of the variety (results are shown in Table 1 and Table 2, column 5).

[0081] Two, according to the typing method established in embodiment 2, detect the genotype of each rice variety (results are shown in Table 1 and Table 2, the 4th row).

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Abstract

The invention discloses a phosphoglyceric acid mutase gene segment capable of improving the yield of paddy rice and application of the phosphoglyceric acid mutase gene segment. A method for screeningpaddy rice with different yields and properties can comprise the following steps: (1) detecting a gene type based on a specific gene segment of paddy rice to be detected, wherein the specific gene segment is located in a paddy rice genome, is OsIPGAM1 and has two allelic forms including OsIPGAM1_a and OsIPGAM1_b, the OsIPGAM1_a is shown as a sequence 1 in a sequence table and the OsIPGAM1_b is shown as a sequence 2 in the sequence table; (2) carrying out following judgment: judging that the average yield of a paddy rice population with the gene type of OsIPGAM1_b homozygous type is higher thanthe average yield of a paddy rice population with the gene type of OsIPGAM1_a homozygous type at different geographic locations and under the same growth condition. An experiment proves that the method disclosed by the invention can be used for screening the paddy rice with different individual plant yield characters; the method is simple to operate and high in accuracy, and has important application value in seed breeding of the paddy rice.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a specific genome fragment and a pair of specific primers for universally cultivating high-yielding rice, in particular to a phosphoside oleate mutase gene fragment for improving rice yield and its application. Background technique [0002] Rice (Oryza sativa L.) is the staple food of more than half of the world's population. As the global population increases and living standards improve, there is an increasing need to breed broad-spectrum, high-yielding rice varieties. Finding out the genome fragments and identification methods that can improve the high yield of rice from various existing rice materials is conducive to the targeted selection, tracking and confirmation of rice varieties with high yield traits in the breeding process. [0003] A basic goal of rice breeding is to obtain rice varieties that grow well under a wide range of geographical conditions. The current breeding m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/29C12N15/11
CPCC07K14/415C12Q1/6895C12Q2600/13
Inventor 鲁迎青
Owner INST OF BOTANY CHINESE ACAD OF SCI
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