Method of Controlling Botrytis Botrytis of Tomato Using Biocontrol Agent
A technology of tomato gray mold and biocontrol agent, which is applied in the field of tomato gray mold prevention and control, can solve the problems of antagonism and pollution, and the poor control effect of biocontrol agent, so as to reduce the burden, increase the added value of products, and improve the quality of products. quality effect
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Embodiment 1
[0033] Utilize the method for preventing and treating tomato gray mold with biocontrol agent, it may further comprise the steps:
[0034] Add the biocontrol agent to 100 times the weight of water, stir evenly, place it at 30°C for 3 hours, and then spray 20ml per plant on the tomato, and repeat the spraying once a week later.
[0035] The tomato is in the early stage of gray mold disease;
[0036] The biocontrol agent is prepared according to the following process:
[0037] Streak inoculation of Trichosporium yeast on YPDA medium and culture to obtain a single colony; pick a single colony and inoculate it on YPD liquid medium for culture, culture at 30°C for 24 hours, and obtain seed liquid for use;
[0038] Formula of YPD medium: glucose 20g, peptone 20g, yeast extract powder 10g, add distilled water to 1000mL;
[0039] Formula of YPDA medium: glucose 20g, peptone 20g, yeast extract powder 10g, agar 20g, add distilled water to 1000mL;
[0040] Streak inoculation of Trichod...
Embodiment 2
[0046] Utilize the method for preventing and treating tomato gray mold with biocontrol agent, it may further comprise the steps:
[0047] Add the biocontrol agent to 50 times the weight of water, stir evenly, place it at 28°C for 5 hours, and then spray 10ml per plant on the tomato, and repeat the spraying once a week later.
[0048] The tomato is in the early stage of gray mold disease;
[0049] The biocontrol agent is prepared according to the following process:
[0050] Streak inoculation of Trichosporium yeast on YPDA medium and culture to obtain a single colony; pick a single colony and inoculate it on YPD liquid medium for culture, culture at 30°C for 24 hours, and obtain seed liquid for use;
[0051] Formula of YPD medium: glucose 20g, peptone 20g, yeast extract powder 10g, add distilled water to 1000mL;
[0052] Formula of YPDA medium: glucose 20g, peptone 20g, yeast extract powder 10g, agar 20g, add distilled water to 1000mL;
[0053] Streak inoculation of Trichode...
Embodiment 3
[0059] Tomato gray mold field control:
[0060] The tomato variety was Yukang No. 4, and it was planted in rows in greenhouses, with a control spacing of 40×50 cm. The tomato seedlings were planted on January 7, and the biocontrol agent was sprayed on February 3 at the early stage of Botrytis cinerea, and the biocontrol agent was sprayed again on February 10, all spraying at an amount of 20ml per plant.
[0061] Groups: Experimental group: Example 1; Control group 1: use Trichoderma konshii-Tychosporium mixed fermentation liquid, the rest is the same as in Example 1; Control group 2: Use Pseudomonas stutzeri culture liquid, the rest is the same as the implementation Example 1; Control group 3: Trichoderma konshii and Trichosporium yeast were fermented separately, Trichoderma konshii fermentation broth, Trichosporium yeast fermentation broth and Pseudomonas stutzeri culture fluid were mixed to prepare bacterial powder, and the rest were implemented in the same way example 1. ...
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