Application of patchoulone in the preparation of drugs for preventing and treating porcine reproductive and respiratory syndrome

A technology for respiratory syndrome and patchouli ketone, which is applied in the direction of pharmaceutical formulations, medical preparations containing active ingredients, antiviral agents, etc., can solve the problem of unpatented and unseen patchouli ketone anti-pig reproductive and respiratory syndrome To achieve good antiviral effect, clear medicinal ingredients and mechanism of action, and high biological safety

Active Publication Date: 2022-08-02
SOUTH CHINA AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there is no research report on the anti-porcine reproductive and respiratory syndrome virus effect of patchouli ketone, and there is no published related patent

Method used

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  • Application of patchoulone in the preparation of drugs for preventing and treating porcine reproductive and respiratory syndrome
  • Application of patchoulone in the preparation of drugs for preventing and treating porcine reproductive and respiratory syndrome
  • Application of patchoulone in the preparation of drugs for preventing and treating porcine reproductive and respiratory syndrome

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: Extraction of Patchoulone

[0025] Patchouli whole herb 50kg, steam distillation to obtain 1.78kg of pale yellow oil, rectification under reduced pressure, under 1.3KPa, to obtain 159-163 ℃ of fractions 345g, carry out silica gel column chromatography, with petroleum ether ( 30-60 ℃)-diethyl ether gradient elution, combined 10% diethyl ether-petroleum ether eluent, and then subjected to silica gel column chromatography to obtain colorless needle crystals (260mg), melting point 32.5-33 ℃, by mass spectrometry and nuclear magnetic resonance Hydrogen spectrum analysis identified patchoulone.

Embodiment 2

[0026] Example 2: Cytotoxicity of patchoulone on Marc-145 determined by CCK-8 method

[0027] After the Marc-145 cells grow to a monolayer in the 96-well cell culture plate, the medium is discarded, washed twice with PBS (phosphate buffered saline), and 100 μL of DMEM cell maintenance solution containing 2% fetal bovine serum is added to dilute it. different concentrations of patchoulone, and three replicate wells were made for each concentration. After continuing to incubate at 37°C for 48h, add 10 μL of LCCCK-8 (Japan Dojin Chemical) reagent to each well, incubate at 37°C for about 2h, and detect the absorbance value at a wavelength of 450nm. from figure 1 It can be seen that patchoulone does not inhibit the proliferation of Marc-145 cells at a concentration of 10 μg / mL.

Embodiment 3

[0028] Example 3: Determination of the inhibitory effect of patchoulone on the proliferation of porcine reproductive and respiratory syndrome virus RNA in Marc-145 cells by real-time fluorescence quantitative PCR

[0029] After the Marc-145 cells grow to a monolayer in a 6-well cell culture plate, the medium is discarded, washed twice with PBS (phosphate buffered saline), and diluted with DMEM cell maintenance solution containing 2% fetal bovine serum. Including 100TCID 50 of porcine reproductive and respiratory syndrome virus (PRRSV), 600 μL / well, incubated at 37°C for 2 h, discarded the virus supernatant, washed twice with PBS, and added 12, 6, and 3 μg / mL of patchoulone, 2 mL / well, respectively. . In the experiment, a normal control group (without test drug, without PRRSV) and a PRRSV control group (without test drug) were set at the same time, and three parallels were set for each test concentration. The cells were cultured at 37°C until 48 h after infection. After obse...

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PUM

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Abstract

The invention discloses the application of patchoulone in preparing medicine for preventing and treating porcine reproductive and respiratory syndrome. Patchoulone was extracted from the traditional Chinese medicine Patchouli. It was confirmed by immunofluorescence microscopy, RT-PCR and Western blot that patchoulone had good anti-PRRSV activity on Marc-145 cells, and could be used as an active ingredient to prepare Drugs for the prevention and treatment of porcine reproductive and respiratory syndrome.

Description

technical field [0001] The invention relates to the field of veterinary medicine, and more particularly relates to the use of patchoulone in preparing a medicine for preventing and treating porcine reproductive and respiratory syndrome. Background technique [0002] Porcine reproductive and respiratory syndrome (PRRS), commonly known as "porcine blue-ear disease", is caused by porcine reproductive and respiratory syndrome virus (PRRSV). A highly contagious disease with a major characteristic. The disease can be transmitted both vertically and horizontally. It can cause fever, anorexia, abortion, premature birth, mummified fetus and weak litter in pregnant sows, as well as various diseases of the respiratory system of piglets and finishing pigs. huge loss. At present, the prevention and control of PRRS is mainly through vaccine immunization, and there is no effective drug approved for the treatment of the disease. However, due to the high variability of PRRSV, the clinical...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/366A61P31/14
CPCA61K31/366
Inventor 陈建新田鸽李志萍曾振灵张桂红汤有志邱电张明昕吴倩倩
Owner SOUTH CHINA AGRI UNIV
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