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Method for extracting microalgae oil with algicidal bacteria

A technology of algae-dissolving bacteria and microalgae oil, which is applied in the direction of fat oil/fat production and fat production, which can solve the problems of energy consumption, high recovery cost, low energy density, etc., and achieve high oil extraction rate, low equipment requirements, and easy operation simple effect

Inactive Publication Date: 2018-07-27
PEKING UNIV SHENZHEN GRADUATE SCHOOL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example: the mechanical method can completely break up the cells, which has the advantages of not being limited by the type of microalgae and not polluting oil products, but this process consumes a lot of energy, generally higher than 400MJ / kg, and there are certain limitations in scale application. Limitations: Although the chemical method has high breaking efficiency, chemical reagents are used in the breaking process, which is likely to cause oil pollution and high recycling costs; compared with traditional mechanical and chemical methods, the enzymatic method has low energy density and environmental protection. It is friendly, and the enzyme reaction has the advantages of high selectivity and less by-products, but the enzyme needs to be separated and purified, and its pretreatment will greatly increase the production cost of biodiesel, thus limiting its large-scale application

Method used

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specific Embodiment 1

[0016] Specific Example 1: The present invention relates to a method for extracting oil from microalgae with algophilic bacteria, in particular to a method for extracting oil from Monopodella algae with algalytic bacteria Aeromona hydrophila.

[0017] The method that the present invention extracts oil from the algae-dissolving bacterium Aeromona hydrophila is realized through the following steps:

[0018] 1. Collect the supernatant of the algae-lytic bacterium Aeromona hydrophila: put the Aeromona hydrophila cultured for 24 hours into the centrifuge for centrifugation, the centrifugal speed is 3500rpm, and the centrifugation time is 5min to obtain the supernatant of Aeromona hydrophila;

[0019] 2. Prepare the mixed solution: collect and cultivate the Monopodella hydrophila for 20 days, mix the Aeromona hydrophila supernatant obtained in step 1 with the Monopodella algae liquid, the mixing volume ratio is 1:1, place it in a light-proof shaker, and Mix for 72 hours at a tempera...

specific Embodiment 2

[0022] Specific Example 2: The present invention relates to a method for extracting oil from microalgae with algicidal bacteria, in particular to a method for extracting oil from Chlorella with algophilic bacteria Bowmanella denitrificans.

[0023] The present invention extracts the method for grease from chlorella with algae-dissolving bacterium Bowmanella denitrificans, is to realize through the following steps:

[0024] 1. Collect the supernatant of Bowmanella denitrificans, an algalytic bacterium: add the Bowmanella denitrificans cultured for 24 hours into a centrifuge for centrifugation, control the centrifugal speed at 3500 rpm, and separate for 5 minutes to obtain the supernatant of Bowmanella denitrificans;

[0025] 2. Prepare the mixed solution: collect and cultivate the chlorella for 20 days, mix the Bowmanella denitrificans supernatant obtained in step 1 with the chlorella algae liquid, the volume ratio is 1:1, place it in a light-proof shaker, and keep it at a tempe...

specific Embodiment 3

[0028] Specific Example 3: The present invention relates to a method for extracting oil from microalgae with algicidal bacteria, in particular to a method for extracting oil from Nannochloropsis with algicidal bacteria Kordia sp.

[0029] The present invention extracts the method for grease from Nannochloropsis with algae-dissolving bacterium Kordia sp, is to realize through the following steps:

[0030] 1. Collect the supernatant of the algae-lytic bacteria Kordia sp: put the Kordia sp bacterial liquid cultured for 24 hours into a centrifuge for centrifugation, control the centrifugal speed at 3500rpm, and the separation time is 5min to obtain the supernatant of Kordia sp;

[0031] 2. Prepare the mixed solution: collect the Nannochloropsis that has been cultured for 15 days, mix the Kordia sp supernatant obtained in step 1 with the Nannochloropsis algae liquid, the mixing volume ratio is 1:1, and place it in a dark shaker , mixing for 72 hours at a temperature of 30° C. and a...

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PUM

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Abstract

The invention belongs to the fields of renewable energy sources and biochemical industry, and specifically relates to a method for extracting microalgae oil with algicidal bacteria. The method comprises the following steps: collection of an algicidal bacteria supernatant, preparation of a mixed solution, obtaining of an organic phase, obtaining of the microalgae oil by distillation, etc. The method provided by the invention solves the technical problems of high energy consumption and easy pollution to oil products for a conventional microalgae cell disruption technology, and is an oil extraction method with the advantages of high efficiency, energy conservation, economy, low cost and high oil product purity.

Description

technical field [0001] The invention relates to a method for extracting oil from microalgae by using algae-dissolving bacteria, which belongs to the field of renewable energy and the field of biochemical industry. Background technique [0002] With the depletion of petrochemical resources and the seriousness of environmental pollution, the development of clean and sustainable bioenergy has attracted widespread attention. Microalgae has the characteristics of high oil content, short growth cycle, and high yield per unit area. It has become a research hotspot of bioenergy at home and abroad in recent years. Encapsulated in microalgae cells, it is difficult to extract, which directly affects the economic cost and yield of oil extraction, and is not conducive to large-scale production and application. Therefore, the research and development of high-efficiency and energy-saving cell crushing technology is an unresolved problem in the microalgae oil extraction process. question. ...

Claims

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Application Information

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IPC IPC(8): C11B1/04
CPCC11B1/025
Inventor 崔岩邓春芳梁园梅成家杨李静
Owner PEKING UNIV SHENZHEN GRADUATE SCHOOL