Applications of compound Sorbicillin in preventing and treating candida albicans
A Candida albicans and compound technology, applied in the field of biomedicine, can solve problems such as research on the compound sorbicillin that has never been seen before, and achieve the effect of promoting development, low toxicity and promoting development
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[0020] The fermentation extraction of embodiment 1 actinomycetes bacterial strain and the preparation of compound sorbicillin
[0021] The actinomycete strain SCSIOT05 was isolated from the seabed sediments (E94°3364′, S1°4256′) at 4617m in the Indian Ocean, and the 16SrDNA gene sequence analysis confirmed that the strain was Streptomyces olivaceusSCSIO T05. The gene sequence was saved to GenBank (accession number MF429815).
[0022] The strain Streptomyces olivaceus SCSIOT05 grown on ISP2 solid medium was inoculated into 1L Erlenmeyer flasks (10 flasks) containing 200mL ISP2 liquid medium, and the culture medium was fermented on a shaker, and fermented at 28°C and 200rpm for two days to obtain seeds. The seeds were inoculated and fermented in a 65L fermenter containing 40LRA medium, and the culture conditions were as follows: temperature 28°C, rotational speed 200rpm, sterile air 20L / min, pressure 0.1Mpa. Sampling 50mL every 12h, butanone extraction and HPLC detection to obs...
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