Applications of compound Sorbicillin in preventing and treating candida albicans

[0020] Example 1 Fermentation extraction of actinomycete strains and preparation of compound sorbicillin

[0021] Actinomycetes strain SCSIOT05 was isolated from the bottom sediments (E94°3364', S1°4256') of the Indian Ocean 4617m. The 16SrDNA gene sequence analysis confirmed that the strain was Streptomyces olivaceusSCSIO T05. The gene sequence was saved in GenBank (accession number MF429815).

[0022] The strain Streptomyces olivaceus SCSIOT05 grown on the ISP2 solid medium was inoculated into a 1L Erlenmeyer flask (10 flasks) containing 200 mL of ISP2 liquid medium, and the medium was shaken for fermentation. The seeds were fermented at 28° C. and 200 rpm for two days. The seeds were inoculated in a 65L fermentor containing 40LRA medium for fermentation and culture. The culture conditions were 28°C, 200rpm, sterile air 20L/min, and pressure 0.1Mpa. 50mL was sampled every 12h, butanone was extracted and tested by HPLC to observe the fermentation.

[0023] Among them, the composition of ISP2 solid medium is: 4.0g/L glucose, 4.0g/L yeast extract powder, 10g/L malt extract powder, 30g/L sea salt, 20g/L agar powder, adjust the pH value to 7.4. The composition of RA medium is: 10g/L glucose, 10g/L malt extract powder, 5.0g/L corn flour, 20g/L soluble starch, 10g/L maltose, 2.0g/L CaCO 3 , 30g/L sea salt, adjust the pH to 7.4.

[0024] After 3 days of fermentation, the bacteria were harvested, centrifuged, the mycelium was extracted three times with acetone and concentrated under reduced pressure to obtain an extract (245mg); the supernatant was extracted three times with butanone and concentrated under reduced pressure to obtain an extract (12.1g). According to HPLC analysis As a result, the two parts of the extract were combined, silica gel column chromatography, chloroform-methanol (100:0, 95:5, 90:10, 85:15, 80:20, 70:30, 50:50) eluted to obtain Fr. There are 7 parts in 1-Fr.7, and the medium-pressure Rp-18 reversed-phase column chromatography in part Fr.2 (30:70→100:0, 0→120min), acetonitrile-water elution, to obtain Fr.2-1 to Fr.2-5 five parts, Fr.2-2 part semi-preparative column chromatography, acetonitrile-water elution (5:5→10:0, 0→30min), to obtain the compound sorbicillin (5.0mg, Rf=19.01min ). The structure of the compound is analyzed by mass spectrometry and 1 H. 13 CNMR data and comparison with literature (Zhao P.J., et al. Chem. Biodivers. 2006, 3:337-342) confirmed.

[0025] Example 2 Effect detection test