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A kind of simultaneous detection method of 5-hydroxy coniferaldehyde and 5-hydroxy coniferyl alcohol

A technology of hydroxy coniferyl aldehyde and hydroxy coniferyl alcohol, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problem of instability, inability to detect the content of 5-hydroxy coniferyl alcohol and 5-hydroxy coniferyl aldehyde, and the inability to detect 5-hydroxy coniferyl alcohol and 5-hydroxy coniferyl alcohol -Hydroxyconiferyl aldehyde separation and other issues

Active Publication Date: 2019-10-18
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The structures of 5-hydroxy coniferyl alcohol and 5-hydroxy coniferyl aldehyde are relatively similar, so that their physical and chemical properties are relatively similar, and 5-hydroxy coniferyl alcohol and 5-hydroxy coniferyl aldehyde are unstable at room temperature, so it is currently impossible to combine 5-hydroxy coniferyl alcohol and 5-hydroxy coniferyl alcohol. Hydroxy coniferyl aldehyde is separated, and the content of 5-hydroxy coniferyl alcohol and 5-hydroxy coniferyl aldehyde cannot be detected

Method used

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  • A kind of simultaneous detection method of 5-hydroxy coniferaldehyde and 5-hydroxy coniferyl alcohol
  • A kind of simultaneous detection method of 5-hydroxy coniferaldehyde and 5-hydroxy coniferyl alcohol
  • A kind of simultaneous detection method of 5-hydroxy coniferaldehyde and 5-hydroxy coniferyl alcohol

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preparation example Construction

[0062] In the present invention, the preparation method of the sample to be tested preferably comprises the following steps:

[0063] (a), mixing the substance to be tested with an alcoholic organic solvent, performing ultrasonic extraction and centrifugation in sequence to obtain a supernatant;

[0064] (b) Drying the supernatant obtained in the step (a) with nitrogen gas, then redissolving with an alcoholic organic solvent and filtering with a microporous membrane to obtain the sample to be tested.

[0065] In the present invention, it is preferred to mix the analyte with an alcoholic organic solvent, perform ultrasonic extraction and centrifugation in sequence to obtain a supernatant.

[0066] In the present invention, the volume ratio of the mass of the analyte to the alcoholic organic solvent is preferably 1g:10-20mL, more preferably 1g:12-18mL, more preferably 1g:14-16mL, even more preferably 1g: 15mL.

[0067] In the present invention, the alcoholic organic solvent in...

Embodiment 1

[0096] (1) Drawing of standard curve

[0097] Weigh the standard substances of 5-hydroxy coniferyl aldehyde and 5 mg of 5-hydroxy coniferyl alcohol respectively. Dissolve in 5mL chromatographic grade methanol solution respectively to obtain 5-hydroxy coniferaldehyde standard solution and 5-hydroxy coniferyl alcohol standard solution. Then be diluted into standard solutions of 100 μg / mL, 300 μg / mL, 500 μg / mL and 700 μg / mL respectively, and measure the absorbance of 5-hydroxy coniferaldehyde and 5-hydroxy coniferyl alcohol under corresponding concentrations respectively, draw standard curve, obtain 5 respectively - Standard curve for hydroxyconiferaldehyde and 5-hydroxyconiferyl alcohol.

[0098] The standard curve of the measured 5-hydroxy coniferaldehyde is y 1 =9282.8x 1 -47.372, as in Figure 6 shown, where x 1 is the concentration of 5-hydroxyconiferinal, in mg / mL, y 1 is the absorbance of 5-hydroxyconiferinal, the correlation coefficient R 2 =0.997, the linear range...

Embodiment 2

[0114] Peel and remove the pit from the young Dangshansu pear fruit, cut it into quarters, weigh 2 g of the sample to be tested and mix it with 30 mL of methanol, extract it ultrasonically at 35 °C for 35 min, and then centrifuge it for 10 min at a centrifuge speed of 5500 r / min , take the centrifuged supernatant and dry it with nitrogen, then add 2 mL of chromatographic grade methanol to redissolve. The solution obtained by reconstitution was filtered with an organic filter membrane with a pore size of 0.22 μm, and the obtained filtrate was used as the test solution.

[0115] Using high performance liquid chromatography, the test sample solution is tested under the following chromatographic conditions to obtain the absorbance of 5-hydroxy coniferaldehyde and the absorbance of 5-hydroxy coniferyl alcohol in the test sample. Then according to the standard curve of 5-hydroxy coniferaldehyde and the absorbance of 5-hydroxy coniferaldehyde in the test sample, the concentration of ...

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Abstract

The invention provides a synchronous detection method of 5-hydroxyconiferyl aldehyde and 5-hydroxyconiferyl alcohol. First, the absorbance of the 5-hydroxyconiferyl aldehyde and the absorbance of the5-hydroxyconiferyl alcohol in a sample to be tested are determined by high performance liquid chromatography, wherein the detection wavelength is 243 nm; the high performance liquid chromatography uses gradient elution in which an eluent comprises methanol and water; according to the standard curve of the 5-hydroxyconiferyl aldehyde and the absorbance of the 5-hydroxyconiferyl aldehyde in the sample to be tested, the concentration of the 5-hydroxyconiferyl aldehyde can be obtained; according to the standard curve of the5-hydroxyconiferyl alcohol and the absorbance of the 5-hydroxyconiferyl alcohol in the sample to be tested, the concentration of the 5-hydroxyconiferyl alcohol in the sample to be tested can be obtained. The synchronous detection method can effectively separate the 5-hydroxyconiferyl aldehyde and the 5-hydroxyconiferyl alcohol, and can separately detect the concentration of the 5-hydroxyconiferyl aldehyde and the concentration of the 5-hydroxyconiferyl alcohol.

Description

technical field [0001] The invention relates to the field of substance separation and detection, in particular to a method for synchronous detection of 5-hydroxy coniferaldehyde and 5-hydroxy coniferyl alcohol. Background technique [0002] Both 5-hydroxyconiferyl alcohol and 5-hydroxy coniferyl alcohol are key intermediate metabolites in the process of pear secondary metabolism to form lignin. By studying the content changes of 5-hydroxy coniferyl alcohol and 5-hydroxy coniferyl alcohol, it is conducive to better research Metabolic pathways of pears. But a kind of method that can separate 5-hydroxy coniferaldehyde and 5-hydroxy coniferyl alcohol in pears and other plants, and detect the content of 5-hydroxy coniferyl aldehyde and 5-hydroxy coniferyl alcohol in pears or other plants has not been found yet. [0003] At present, there are few studies on 5-hydroxyconiferyl alcohol and 5-hydroxyconiferaldehyde in the field of chemistry or biology. The structural formula of 5-h...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/38
Inventor 蔡永萍李国辉程曦江桃山朱英鹏金青林毅
Owner ANHUI AGRICULTURAL UNIVERSITY
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