Oxygen-rich gel gent for promoting wound healing, preparation method and applications thereof

An oxygen-enriched gel and wound surface technology, which is applied in the direction of medical preparations, drug combinations, and pharmaceutical formulas with non-active ingredients, can solve the problems of ineffective tissue repair and perfluorinated compounds that cannot be metabolized by tissues. It is not easy to achieve Effect of immunogen response, improvement of sterilization efficiency and production efficiency, and promotion of repair

Active Publication Date: 2018-08-03
北京颐方生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Chinese patent CN102949709B discloses the preparation and application of an external gel for treating diabetic foot. The oxygen-carrying material in the patent is a perfluorinated compound. Can not be metabolized by tissues, its application has certain limitations
Patent CN103041438B discloses a wet antibacterial hydrogel dressing for treating diabetic foot ulcers, which uses nano-silver as a bactericidal component and alginate as a gel component, but the hydrogel dressing has no obvious effect on tissue repair
Patent CN103083226B discloses the preparation of fibroblast growth factor-1 modified gel and its application in the treatment of diabetic foot. The gel matrix is ​​carbomer, and the gel has no obvious effect on tissue repair; thus It can be seen that the above-mentioned technical solutions all have certain limitations.

Method used

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  • Oxygen-rich gel gent for promoting wound healing, preparation method and applications thereof
  • Oxygen-rich gel gent for promoting wound healing, preparation method and applications thereof
  • Oxygen-rich gel gent for promoting wound healing, preparation method and applications thereof

Examples

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Embodiment 1

[0040] This embodiment provides a preparation of Nereis worm extracellular hemoglobin extract, including:

[0041] 1. Disinfection of clamworms: Take clamworm adults that have not produced sex cells with reddened color. This experiment uses Tylorrhynchus heterochaetus (Tylorrhynchus heterochaetus) commonly known as worms 2Kg, and starvation culture in filtered flowing sea water for 12 hours. After starvation treatment, Nereist worms are placed in filtered and purified seawater, and ozone is filled into the water with an ozone generator to make the ozone concentration in the water ≥ 0.3 mg / L, and maintain this concentration for 30 minutes.

[0042] 2. Tissue homogenization / centrifugation: Put the sterilized clamworm into 1 L of saline solution (mass fraction 0.1% CaCl 2 and 0.2% MgCl 2 , pH is 6.5), and crushed in a tissue pulverizer for 30s; put the pulverized tissue slurry into a high-speed centrifuge for high-speed centrifugation, the centrifugation conditions are: temperat...

Embodiment 2

[0047] Example 2 A gel that increases the oxygen content of wound tissue

[0048] This embodiment provides a gel for increasing the oxygen content of wound tissue, each 1ml of the gel contains 0.33ml of Nereis extracellular hemoglobin extract prepared in Example 1, 30mg of sodium alginate, 40mg of gelatin, carboxymethyl Chitosan 5mg.

[0049] The preparation method of described gel comprises the following steps:

[0050] 1. Solution preparation and sterilization:

[0051] ① Take pharmaceutical grade sodium alginate and make 90mg / ml sodium alginate solution, add the weighed sodium alginate into sterile deionized water, the pH is 6.5, and stir magnetically at 50-60°C until Completely dissolved to obtain sodium alginate viscous liquid.

[0052] ② Take pharmaceutical grade gelatin to prepare a gelatin solution of 240 mg / ml, add the weighed gelatin into deionized water with a pH of 6.0, place it under the condition of 50-60°C, and magnetically stir until it is completely dissolv...

Embodiment 3

[0058] This embodiment provides a gel for increasing the oxygen content of the wound tissue. Each 1ml of the gel contains 0.33ml of the nereis extracellular hemoglobin extract prepared in Example 1, 30mg of sodium alginate, 40mg of gelatin, and 4mg of carboxymethyl base chitosan.

[0059] The preparation method of described gel comprises the following steps:

[0060] 1. Solution preparation:

[0061] ① Take sodium alginate to prepare a 90mg / ml sodium alginate solution, add the weighed sodium alginate into deionized water, the pH is 6.5, and stir magnetically at 50-60°C until it is completely dissolved to obtain seaweed Sodium acid viscous liquid;

[0062] ② Take gelatin to prepare a 240mg / ml gelatin solution, add the weighed gelatin into deionized water, the pH is 6.0, place it under the condition of 50-60°C, stir it with magnetic force until it is completely dissolved, and obtain an aqueous gelatin solution;

[0063] ③ Weigh the pharmaceutical grade water-soluble chitosan ...

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Abstract

The invention relates to an oxygen-rich gel gent for promoting wound healing, a preparation method and applications thereof, wherein the oxygen-rich gel gent contains a Nereidae extracellular hemoglobin extraction solution, and the Nereidae extracellular hemoglobin extraction solution comprises the following components: Nereidae extracellular hemoglobin with a mass fraction of 0.1-10.0%, 5-20 mmol / L Ca<2+>, and 10-100 mmol / L Mg<2+>. According to the present invention, the gel agent prepared by using the Nereidae extracellular hemoglobin extraction solution has functions of sterilizing, moisture retention, wound exudate absorption and the like, and can promote the healing of refractory wounds, burn wounds, and contaminated wounds such as diabetic foot and bedsore wounds.

Description

technical field [0001] The present invention relates to the technical field of medical equipment and its preparation, in particular to a clam worm extracellular hemoglobin extract, a method for preparing an oxygen-enriched gel capable of promoting wound healing, and its application in refractory wounds and polluted wounds such as Application in diabetic foot, decubitus wound and burn wound. Background technique [0002] Tissue ulcerative necrosis due to persistent tissue ischemia, hypoxia, and malnutrition is a common problem in nursing. Common symptoms include foot tissue ulceration caused by diabetes and pressure ulcers (pressure sores, decubitus ulcers) caused by long-term pressure on local tissues. Diabetic foot is one of the main chronic complications of diabetes and the main reason for non-traumatic amputation, which seriously affects the quality of life of diabetic patients. There are currently more than 200 million diabetic patients in the world, of which 10% to 15%...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/64A61K9/06A61K47/36A61K47/42A61P17/02A61L26/00
CPCA61K9/0014A61K9/06A61K47/36A61K47/42A61L26/0023A61L26/0038A61L26/0066A61L26/008A61L2300/30A61L2300/404A61K35/63C08L5/04C08L5/08
Inventor 石清东王正元陈赫岩王姣
Owner 北京颐方生物科技有限公司
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