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High-density molecular-marker-assisted pyramiding breeding method based on core genealogy varieties

A molecular marker-assisted, polymerization breeding technology, applied in the fields of botany equipment and methods, biochemical equipment and methods, plant genetic improvement, etc. Effects of breeding, reducing blindness and randomness, reducing breeding costs

Active Publication Date: 2018-08-07
RICE RES INST GUANGDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] The present invention proposes a new hybrid breeding strategy, aiming to solve the problems of wide-spreading, high investment, and long cycle in traditional hybrid breeding, so as to realize refined and rapid directional breeding

Method used

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  • High-density molecular-marker-assisted pyramiding breeding method based on core genealogy varieties
  • High-density molecular-marker-assisted pyramiding breeding method based on core genealogy varieties
  • High-density molecular-marker-assisted pyramiding breeding method based on core genealogy varieties

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Experimental program
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Effect test

Embodiment 1

[0048] 1. Determine the genome segment information related to the traits of the core pedigree varieties.

[0049] Using the research method in section (4) of the background technology, more than 1,000 genome segments related to traits of the South China rice core pedigree variety "Huanghuazhan" were determined. In the subsequent analysis and processing, these genome segments are considered as the smallest recombination unit and are closely related to breeding.

[0050] The literature [Pedigree-based analysis of derivation of genome segments of anelite rice reveals key regions during its breeding. Plant Biotechnology Journal, 2016, 14(2): 638-648] introduced in detail the method of population resequencing combined with recombination breakpoint map (bin map) analysis, QTL mapping, and cloned gene information to determine the research methods of the associated segments of "Huang Huazhan" traits. Examples of related results are as follows figure 1 shown.

[0051] 2. Obtain the ...

Embodiment 2

[0098] In this example, the new-generation core pedigree variety Huangguangyouzhan derived from Huanghuazhan was used as the female parent, the introduced variety 1 was Wuguangzhan, and the introduced variety 2 was Fengyue Huazhan. Huangguangyouzhan is highly resistant to rice blast, the rice quality is not high-quality, and moderately sensitive to bacterial blight; Wuguangzhan is resistant to rice blast, with national standard high-quality grade 3 and provincial standard high-quality grade 2, and moderate resistance to bacterial blight; Fengyuehua Accounting: medium resistance to rice blast, rice quality national standard provincial standard high-quality grade 2, medium resistance to bacterial blight. The purpose of breeding is to maintain the high yield and rice blast resistance of Huangguangyouzhan, introduce the high-quality rice traits and bacterial blight resistance of Wuguangzhan and Fengyue Huazhan, and select high-yield, high-quality, and disease-resistant conventional...

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Abstract

The invention discloses a high-density molecular-marker-assisted pyramiding breeding method based on core genealogy varieties. The method disclosed by the invention comprises the steps: by utilizing parting results of high-density molecular markers, in combination with related markers of important characters such as stress resistance, quality, yield and the like of the core genealogy varieties andgenome zone information, a hybrid breeding strategy which takes a construction core genealogy variety as a main body and introducing excellent genes and characters of other varieties is established;existing high-quality variety resources are integrated to achieve quick and directional breeding. The method disclosed by the invention greatly reduces seed selection blindness and randomness, improves breeding efficiency and reduces breeding cost. Whole-genome high-density SNP marking is utilized to perform assistant selection, so that the choosing efficiency is improved; meanwhile, obtained derived variety phenotype information and genetic information bring convenience to following pyramiding breeding. In a pyramiding breeding process, more high-quality genetic resources are introduced, an optimal fragment combination pattern is continuously optimized and improved, and breeding of varieties in which more protogene and fragments are pyramided is accelerated.

Description

technical field [0001] The invention belongs to the technical field of rice breeding, in particular to a high-density molecular marker-assisted polymerization breeding method based on core pedigree varieties. Background technique [0002] (1) Single nucleotide polymorphism (single nucleotide polymorphism, SNP), mainly refers to the DNA sequence polymorphism caused by a single nucleotide variation at the genome level. It is the most common type of heritable variation in organisms, accounting for more than 90% of all known polymorphisms. The number of SNPs is large and widely distributed, suitable for rapid and large-scale screening, easy for genotyping, and has a wide range of applications in the field of molecular breeding. The high-density molecular markers used in the present invention usually refer to SNP unless otherwise specified. It should be understood that the use of other molecular markers is also applicable to the method of the present invention. [0003] (2) Nex...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H6/46A01H1/04A01H1/02C12Q1/6895
Inventor 王重荣周少川周德贵李宏黄道强赖穗春王志东陈宜波吴玉坤
Owner RICE RES INST GUANGDONG ACADEMY OF AGRI SCI
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