Sunflower disc water extractive with xanthine oxidase inhibitory activity, preparation method and application thereof
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A technology of xanthine oxidase and inhibitory activity, applied in the field of medicine, can solve the problems of less research on medicinal value and the like
Pending Publication Date: 2018-08-10
JILIN UNIV
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As its by-product resources, flower discs are often used as livestock feed, and there are few studies on its medicinal value
Method used
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Embodiment 1
[0020] Example 1: Preparation of Sunflower Disc Water Extract and Its XO Inhibitory Activity in Vitro
[0021] 1. Preparation of water extract of sunflower disc
[0022] (1) Sample pretreatment: grind the sunflower disc grinder, pass through a 60-mesh sieve, and collect the crude product for later use.
[0023] (2) Weigh 5g of the crude product from step (1), put them into conical flasks respectively, add 100 mL of distilled water to each conical flask, and set them at different temperatures (50, 60, 70, 80, 90, 100°C) Extract by shaking water for 2 hours, centrifuge at 5000rpm / min for 10min to obtain filtrate Ⅰ; collect the precipitate, add 100mL of distilled water, extract by shaking water for 1h at the same temperature, and centrifuge at 5000rpm / min for 10min to obtain filtrate II; collect the precipitate, add 50mL of distilled water, Shake water extraction at the same temperature for 1 h, centrifuge at 5000 rpm / min for 10 min to obtain filtrate III; combine the filtrates....
Embodiment 2
[0035]Example 2: In vivo uric acid-lowering and safety effects of sunflower disc water extract
[0036] 1. Experimental materials
[0037] Animals: Kunming rats, male, body weight (20±2g), provided by the experimental technology platform of the College of Life Sciences, Jilin University; administration samples: normal blank group (distilled water), hyperuricemia model group, positive drug group (allopurinol ), the group of the present invention (water extract of sunflower disc, low, middle, high, dosage groups).
[0038] 2. Experimental method
[0039] Get 60 male mice, after adapting to one week, get rid of unqualified mice, be randomly divided into normal blank group, hyperuricemia model group, positive drug group and the present invention group (low, middle, high dose group), 9 per group. Except the normal group, hypoxanthine (500 mg / kg) was intragastrically administered to each group, and oxonate potassium (100 mg / kg) was injected intraperitoneally at the same time to e...
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Abstract
The invention discloses a sunflower disc water extractive with the xanthine oxidase inhibitory activity, a preparation method and application thereof, and belongs to the technical field of medical science. The method comprises the steps that sunflower discs are dried, ground and screened by a 60-mesh sieve to obtain a crude product; 3-8 g of the crude product is added into 100 mL distilled water,oscillation water extraction is performed for 1-3 h under the temperature of 50-100 DEG C, centrifuging is performed under the rate of 3,000-8,000 rpm / min for 10-20 min, and a filtrate I is obtained;precipitates are collected, 100 mL of distilled water is added, oscillation water extraction is performed under the same condition, and centrifuging is performed to obtain a filtrate II; precipitatesare collected, 50 mL of distilled water is added, oscillation water extraction is performed under the same condition, and centrifuging is performed to obtain a filtrate III; the filtrates I, II and III are combined; ultrafiltration is performed on the filtrates, then, the filtrates pass through a filter membrane of the size being 0.45 micrometer, and freeze drying is performed to obtain the sunflower disc water extractive; the yield is 25.00-39.20%. The sunflower disc water extractive can be applied for preparing medicine or food for reducing uric acid and promoting mouse kidney health.
Description
technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a water extract of sunflower disc with xanthine oxidase inhibitory activity, a preparation method and application thereof. Background technique [0002] Sunflower (helian thus annuus L.) is an annual plant of the genus Helianthus in the Compositae family. It is native to North America and is now one of the world's oil crops, widely planted around the world. It is not only an economical edible plant, but also a medicinal plant worthy of development and utilization. It is sweet in taste, warm in nature, and non-toxic. [0003] Sunflower is widely planted in my country. As an important oil crop, sunflower has a very important application in the food industry. As its by-product resources, flower discs are often used as livestock feed, and there are few studies on its medicinal value. Contents of the invention [0004] The object of the present invention is to provide...
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