Anti-torch-IgM antibody spectrum chip, preparation method of chip and TORCH detection kit

A technology of antibody spectrum and kit, applied in the field of anti-torch-IgM antibody spectrum chip and its preparation, TORCH detection kit, which can solve the problems of high cost, high price, and inability to quantify, and achieve high accuracy and stability Good performance, the effect of reducing the amount of use

Active Publication Date: 2018-08-14
SHENZHEN BLOT BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

ELISA reagents are widely used in ordinary laboratories because of their advantages such as stability, high sensitivity, strong specificity, and low cost, but they are generally used for qualitative, not quantitative
However, IFT is generally only for the detection of a single indicator, and the colloidal gold method is generally only qualitative or semi-quantitative. Although the gene chip technology is effective, it is expensive and generally expensive

Method used

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  • Anti-torch-IgM antibody spectrum chip, preparation method of chip and TORCH detection kit
  • Anti-torch-IgM antibody spectrum chip, preparation method of chip and TORCH detection kit
  • Anti-torch-IgM antibody spectrum chip, preparation method of chip and TORCH detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Embodiment 1, the preparation of anti-torch-IgM type antibody spectrum chip kit

[0048] 1. Antigen coating:

[0049] 1) The array design of the chip and the specific distribution of antigens and reference points are as follows (not limited to the following arrangement design):

[0050]

[0051] 2), specific coating process:

[0052] Antigen and relevant reference point proteins are first diluted as follows:

[0053] PC, NC, S1, S2, S3, S4, S5, and EC points in the array are respectively coated with 2μg / ml, 0.01μg / ml, 0.5μg / ml, 1μg / ml, 2.5μg / ml, 5μg / ml , 10 μg / ml, 2.5 μg / ml human IgM, the dilution buffer is the CB buffer solution of pH9.6 (which contains 0.5% PEG4000, 5% trehalose, 0.02% Captisol, 0.05% Proclin300, and 15 % of glycerol).

[0054] SC dots were coated with 2 μg / ml goat anti-IgM antibody, and the dilution buffer was CB buffer at pH 9.6.

[0055] Loc spots were coated with 2 μg / ml human IgM, and the dilution buffer was CB buffer at pH 9.6.

[0056]...

Embodiment 2

[0070] Example 2, Evaluation of the accuracy of the anti-torch-IgM antibody profile chip kit

[0071] 1. 10 cases of anti-toxoplasma IgM positive serum and 10 cases of anti-toxoplasma IgM negative serum determined by Diasorin company ELISA kit screening were tested with the anti-torch-IgM antibody spectrum chip prepared in Example 1, and the results were shown in Table 1 (+ means positive, - means negative).

[0072] Anti-torch-IgM antibody spectrum chip test anti-toxoplasma gondii IgM serum result prepared by table 1 embodiment 1

[0073]

[0074] 2. Determine anti-rubella virus IgM positive sera and 10 examples of anti-rubella virus IgM negative sera with Diasorin company's ELISA kit screening, and use the anti-torch-IgM antibody spectrum chip test prepared in Example 1, the results are as follows in table 2 ( + means masculine, - means negative).

[0075] The anti-torch-IgM type antibody spectrum chip test anti-rubella virus IgM serum result that table 2 embodiment 1 p...

Embodiment 3

[0100] Embodiment 3, anti-torch-IgM type antibody profile chip kit specificity evaluation

[0101] Compare the specificity of the anti-torch-IgM antibody spectrum chip kit of the present invention with the traditional ELISA kit. Taking the Toxoplasma project as an example, 20 cases of anti-Toxoplasma IgM antibody clinical negative serum were selected, and the anti-torch-IgM antibody spectrum chip prepared in Example 1 was tested simultaneously with the Diasorin ELISA kit. The test data are as follows in Table 11 (“ +" means positive, "±" means weak positive, "-" means negative).

[0102] The anti-torch-IgM type antibody spectrum chip specificity comparison result prepared in Table 11 Example 1

[0103]

[0104] The results showed that the anti-torch-IgM antibody spectrum chip prepared in Example 1 had no false positives in the 20 negative sera tested, while 2 false positives occurred in the 20 negative sera tested by the Diasorin ELISA kit on the market. It shows that the...

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Abstract

The invention belongs to the technical field of biological detection, and discloses an anti-torch-IgM antibody spectrum chip, a preparation method of the chip, a TORCH detection kit and a detection method. The anti-torch-IgM antibody spectrum chip can be used for simultaneously and quantificationally detecting the infection of various related pathogenic microbes of TORCH; by optimizing coating liquid and coating conditions of the chip and sealing stabilizing agents, the stability of the anti-torch-IgM antibody spectrum chip is better; the use period is longer. The TORCH detection kit providedby the invention has the advantages that the consumption of relevant antigens can be effectively reduced; the antigen cost can be reduced; meanwhile, the stable period is longer; the use period is longer; the transportation cost is reduced; the storage and transportation convenience is improved. The TORCH detection method can be used for simultaneously and quantificationally detecting the infection of various related pathogenic microbes of TORCH; the sensitivity is high; the specificity is high.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to an anti-torch-IgM antibody spectrum chip, a preparation method thereof and a kit for TORCH detection. Background technique [0002] TORCH is the pathogen that causes congenital intrauterine infection and perinatal infection and causes perinatal malformation. It is the abbreviation of the English name of a group of pathogenic microorganisms, where T (Toxoplasma) is Toxoplasma gondii, O (Others) is other pathogenic microorganisms, Such as Treponema pallidum, herpes zoster virus, parvovirus B19, Coxsackie virus, etc., R (Rubella.Virus) is rubella virus, C (Cytomegalo.Virus) is cytomegalovirus, H (Herpes.Virus) is herpes simplex Type I / II. TORCH infection is one of the important factors that seriously endanger the health of newborns. It is called TORCH syndrome in perinatal medicine, and its infection is distributed worldwide. In the Chinese population, TOR...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/68G01N21/76
CPCG01N21/76G01N33/569G01N33/68G01N33/56905G01N33/56927G01N33/56983G01N33/56994G01N33/5761G01N2333/19G01N2333/45G01N2469/20
Inventor 张大准肖川张永顶马伟民王洪涛马新民
Owner SHENZHEN BLOT BIOTECH
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