Preparation method for hyaluronic acid self-assembled colloidal particle

A technology of hyaluronic acid and colloidal particles, which is applied in the preparation of hyaluronic acid composite colloidal particles, formulation composition, preparation and drying, can solve the problems of easy degradation, short retention time, poor stability, etc., and achieve good hydrophilicity, The effect of excellent safety

Inactive Publication Date: 2018-08-17
YANBIAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, HA is easily soluble in water, has poor stability, is sensitive to hyaluronidase and free radicals, is easily degraded, has a short retention time in the body, and lacks mechanical strength in water systems, which greatly limits its application. Modification to slow down the degradation rate in the gastrointestinal tract to improve its mechanical strength and anti-degradation properties

Method used

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  • Preparation method for hyaluronic acid self-assembled colloidal particle
  • Preparation method for hyaluronic acid self-assembled colloidal particle
  • Preparation method for hyaluronic acid self-assembled colloidal particle

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1: 50kD HA prepares the method for HA-PDM colloidal particle

[0034] Accurately weigh 0.5g of 50kD HA, put it in 50ml of ultrapure water, stir and dissolve, add 300μl of DM and stir for 2h, use dilute hydrochloric acid to adjust the pH of the mixed solution to 6.30, put it in a round bottom flask, add the initiator KPS (2.5%) solution: 1.5ml, feed nitrogen, stir at 1300rpm / min, heat in a water bath at 70°C, condense and reflux, react for 4h, then cool the solution and put it into a dialysis bag (8000Da), in a phosphate buffer with pH 5.0 After dialysis for 72 hours, the solution was taken out and placed in a watch glass, pre-frozen at -20°C (refrigerator), and dried in a freeze dryer for 48-72 hours. Take it out and grind it, and store it at 4°C.

Embodiment 2

[0035] Embodiment 2: 500kD HA prepares the method for HA-PDM colloidal particle

[0036] Precisely weigh 0.5g of 500kD HA, put it in 50ml of ultrapure water, stir and dissolve, add 500μl of DM and stir for 2h, use dilute hydrochloric acid to adjust the pH of the mixed solution to 6.30, put it in a round bottom flask, add the initiator KPS (2.5%) solution: 2.5ml, feed nitrogen, stir at 1560rpm / min, heat in a water bath at 70°C, condense and reflux, react for 4h, then cool the solution and put it into a dialysis bag (8000Da), in a phosphate buffer with pH 5.0 After dialysis for 72 hours, the solution was taken out and placed in a watch glass, pre-frozen at -20°C (refrigerator), and dried in a freeze dryer for 48-72 hours. Take it out and grind it, and store it at 4°C.

Embodiment 3

[0037] Embodiment 3: 1200kD HA prepares the method for HA-PDM colloidal particle

[0038] Precisely weigh 0.5g of 1200kD HA, put it in 50ml of ultrapure water, stir and dissolve, add 300μl of DM and stir for 2h, use dilute hydrochloric acid to adjust the pH of the mixed solution to 6.30, put it in a round bottom flask, add the initiator KPS (2.5%) solution: 2.5ml, feed nitrogen, stir at 1560rpm / min, heat in a water bath at 70°C, condense and reflux, react for 4h, then cool the solution and put it into a dialysis bag (8000Da), in a phosphate buffer with pH 5.0 After dialysis for 72 hours, the solution was taken out and placed in a watch glass, pre-frozen at -20°C (refrigerator), and dried in a freeze dryer for 48-72 hours. Take it out and grind it, and store it at 4°C.

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Abstract

The invention discloses a preparation method for a hyaluronic acid self-assembled colloidal particle, which comprises formula composition and preparation process. With different molecular weights of hyaluronic acid materials, the method adopts a static electricity-polymerization method to prepare the HA-PDM colloidal particle. According to the invention, with the appropriate method, the electrostatic adsorption of anions and cations and the polymerization reaction of cationic monomer are utilized for preparing the HA-PDM colloidal particle, and the characteristics of hyaluronic acid are kept.The method is easy to operate, and conditions are easy to control.

Description

technical field [0001] The invention relates to a preparation method of hyaluronic acid composite colloid particles, specifically including prescription composition, preparation and drying methods. It belongs to the technical field of polymer materials. Background technique [0002] Hyaluronic acid (HA) is a linear acidic mucopolysaccharide found in all vertebrate organs and blood, and widely distributed in the extracellular matrix (ECM) of soft connective tissue. It is a natural polymer material. Hyaluronic acid has high viscoelasticity, plasticity, super strong water holding capacity, permeability, good biocompatibility and degradability, and it has a wide range of applications in the fields of pharmacy, medicine, food, cosmetics and so on. However, HA is easily soluble in water, has poor stability, is sensitive to hyaluronidase and free radicals, is easily degraded, has a short retention time in the body, and lacks mechanical strength in water systems, which greatly lim...

Claims

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Application Information

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IPC IPC(8): C08J3/00C08L5/08C08L33/14C08F120/34
CPCC08F120/34C08J3/00C08J2305/08C08J2333/14
Inventor 朴明贯常美玲南京华
Owner YANBIAN UNIV
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