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A kind of Alcaligenes faecalis and its application in the degradation and decolorization of azo dyes
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A technology of Alcaligenes faecalis and azo dyes, applied in the field of environmental microorganisms
Active Publication Date: 2021-01-26
WENZHOU UNIV
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However, Alcaligenes faecalis, which has a broad-spectrum effect on the degradation and decolorization of azo dyes, has not been reported yet.
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Embodiment 1
[0032] Example 1 Screening, isolation and identification of Alcaligenes faecalis WZ-010
[0033] 1. Alcaligenes faecalis WZ-010 was isolated from the sludge obtained from the biological treatment tank of Wenzhou Lucheng Printing and Dyeing Factory. Take 1 gram of sludge into a 250 ml sterilized Erlenmeyer flask containing 10 small glass beads, add 100 ml of sterile saline, shake and cultivate on a shaker at 30°C at 150 rpm for 30 minutes, and let stand for 2 hours; take the above bacterial suspension, diluted 10 -5 , 10 -6 , 10 -7 Take 100 microliters and smear it on the LB medium plate (the composition of LB medium, unit g / L: peptone 10, yeast extract 5, NaCl 10, agar 20, pH7.0, sterilized at 121°C for 15 minutes), Each concentration was repeated three times, cultured at 37°C for 48 hours, and a single colony with a large transparent circle was selected for multiple purification, and the purified strain was inoculated on the slant medium, cultured at 37°C for 48 hours, and...
Embodiment 2
[0052] Example 2 Decolorization and Decolorization of Acid Scarlet 3R by Alcaligenes faecalis WZ-010
[0053] (1) Slant culture: Alcaligenes faecalis WZ-010 was inoculated on the slant medium, and cultured at 37° C. for 48 hours. The composition of the slant medium used was (unit g / L): peptone 10, yeast extract 5, sodiumchloride 10, agar 20, pH value 7.0, sterilized at 121° C. for 15 minutes.
[0054] (2) Seed culture: Take a loop of the strain grown on the slant medium with an inoculation loop and insert it into the seed medium, and culture at 37° C. for 24 hours. The seed medium used for seed cultivation consists of (unit g / L): peptone 10, yeast extract 5, sodiumchloride 10, pH 7.0, 100 ml of seed medium in each 250 ml Erlenmeyer flask, sterilized at 121° C. for 15 minutes.
[0055] (3) Degradation and decolorization culture: take 10 milliliters of the cultured seed solution, put it in a 15 milliliter sterile centrifuge tube, centrifuge for 10 minutes at 8000 rpm, discard...
Embodiment 3
[0059] Example 3 Decolorization and Decolorization of Acid Scarlet 3R by Alcaligenes faecalis WZ-010
[0060] Get 10 milliliters of cultivated seed liquids, transfer the collected thalli all into 250 milliliters of Erlenmeyer flasks, wherein 100 milliliters of decolorization medium containing Acid Scarlet 3R are housed, and the concentration of Acid Scarlet 3R is 100 mg / liter, decolorization The pH of the medium is 7.0, and the degradation and decolorization rate reaches 98.76% after static culture at 37° C. for 12 hours.
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Abstract
The invention discloses a bacterium called Alcaligenes faecalis named as Alcaligenes faecalis WZ-010, which is preserved in the General Microorganism Center of China Committee for the Preservation of Microorganisms, and the preservation number is CGMCC No.14108. The invention discloses the application of the bacterial strain in the degradation and decolorization of azo dyes. The steps of the application method are as follows: 1. Cultivation on an inclined plane; 2. Cultivation of seeds; All the bacteria were transferred to the decolorization medium, and the azo dye was decolorized and decolorized under the conditions of temperature 30-45°C and pH value 6.0-9.0 for 4-24 hours, and the decolorization rate reached 92.62%-99.17%. The present invention first obtains the Alcaligenes faecalis which has broad-spectrum degradation and decolorization effects on azo dyes, and it is of great significance to the treatment of dye pollution and the protection of human health.
Description
technical field [0001] The invention relates to the technical field of environmental microorganisms, in particular to Alcaligenes faecalis and its application in the degradation and decolorization of azo dyes. Background technique [0002] Synthetic dyes are widely used in textile printing and dyeing, papermaking, leather, cosmetics, pharmaceuticals and other industries. my country is a major producer of dyes, with an annual production of more than 1.5 million tons of dyes, and 10%-20% of dyes are discharged into the environment along with wastewater every year. middle. There are more than 1,000 kinds of dyes used in the printing and dyeing industry. Due to the needs of textile and other industries, most of the synthetic dyes used in practice are refractory compounds that are resistant to light and oxidation. [0003] There are many kinds of synthetic dyes. According to the basic chemical structure in the molecule, the dyes can be divided into azo dyes, anthraquinone dyes, t...
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