SNP marker affecting yean trait of Huyang sheep (sheep raised in Zhejiang Province) and application of SNP marker

Abstract

The invention belongs to the fields of molecular biotechnology and molecular marker technology, and relates to applications of an SNP molecular marker in researches on yean trait of sheep and in sheepbreeding. The site of the sheep SNP molecular marker is shown as SEQ ID NO.1, and nucleic acid single-base mutation at the 193rd site of the sequence fragment is named as G765A. The SNP molecular marker corresponds to G>A mutation at the 765bp of a coding region sequence of a BMPR-IB gene (accession number: NC-019463.2) on the No.6 sheep chromosome, which is issued, on GeneBank. According to theinvention, by optimizing a dominant allele type of the SNP molecular marker, a genetic progress of yean trait of the Huyang sheep can be accelerated and breeding time of reproduction trait of the Huyang sheep can be shortened, so that reproduction cost is reduced, and economic benefits of mutton sheep breeding industry are effectively improved.

Description

technical field [0001] The invention belongs to the field of molecular biotechnology and molecular marker technology, and in particular relates to the application of a Hu sheep SNP molecular marker in the study of sheep lambing traits and sheep breeding. Background technique [0002] For breeding stock, fecundity is productivity, and fecundity is directly related to the economic effect of mutton sheep industry. Reproductive performance mainly includes the total number of lambs born, the number of live lambs born, birth weight, newborn litter weight, weaned litter weight and other indicators. Low lambing rate is the main bottleneck restricting the development of high-quality and efficient sheep raising industry. It is difficult to quickly improve the reproductive performance of mutton sheep by adopting conventional breeding methods. However, it is very important to study the breeding method of improving lambing rate from the genetic nature. Sheep scientific and technological...

AI Technical Summary

Problems solved by technology

Patent CN104099330A discloses that the allelic mutation occurs at position 713 of the LHβ gene fragment of sheep (breeds include Dorper and Hu sheep), which affects the number of lambs born in Mianyang

[0005] However, there are still some problems in molecular breeding at present, mainly including: the limitation of marker genetic map; the polymorphism frequency of molecular markers linked to breeding target traits is low; The degree of automation is low, and the required equipment and reagents are expensive

However, in gene mapping, due to the limitations of current mapping materials, there is often the problem of inaccurate positioning, and in the technology of QTL mapping of quantitative trait loci, not all target QTLs can be detected, and QTL and There are problems such as the interaction between the environment and the masking of QTLs with large effects on QTLs with small effects

In addition, the RFLP labeling method used in this study also has technical defects such as a large amount of DNA required, complicated detection techniques, and difficulty in applying to large-scale breeding practices.

And these factors have increased the difficulty of finding a new SNP molecular marker

[0006] In addition, some existing molecular markers for lambing traits have different genotypes in their alleles, and the difference in the number of lambs is not particularly significant, which is not particularly ideal for the improvement of the number of lambs

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