Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

White blood cell classification reagent

A white blood cell classification and reagent technology, which is applied in the field of blood cell analysis, can solve the problems of not clear graphic classification, narrow applicable temperature range, etc., and achieves the effect of obvious location demarcation, wide temperature range and convenient use.

Pending Publication Date: 2018-08-17
SHENZHEN DYMIND BIOTECH
View PDF11 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] In view of the above-mentioned deficiencies in the prior art, the purpose of the present invention is to provide a new leukocyte classification reagent, which can be used in a wide temperature range and can divide leukocytes into four cell groups with clear boundaries, aiming to solve the problem of Existing white blood cell classification reagents have problems such as too narrow applicable temperature range and unclear graphic classification

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • White blood cell classification reagent
  • White blood cell classification reagent
  • White blood cell classification reagent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] A reagent for the five classifications of white blood cells, which consists of the following components:

[0047]

[0048] The pH of the reagent prepared according to the above formula is 4.5. The above reagents were tested on the blood sample A on the DF50 automatic blood cell analyzer, and the results were as follows: figure 1 As shown, the white blood cells can be divided into four clear cell groups of lymphocytes, monocytes, neutrophils and eosinophils on the scatter diagram.

Embodiment 2

[0050] A reagent for the five classifications of white blood cells, which consists of the following components:

[0051]

[0052]

[0053] The pH of the reagent prepared according to the above formula is 5.5. The above reagents were tested on blood sample B on the DF50 automatic blood cell analyzer, and the results were as follows: figure 2 As shown, the white blood cells can be divided into four clear cell groups of lymphocytes, monocytes, neutrophils and eosinophils on the scatter diagram.

Embodiment 3

[0055] A reagent for the five classifications of white blood cells, which consists of the following components:

[0056]

[0057] The pH of the reagent prepared according to the above formula is 4.2. The above reagents were tested on blood sample C on the DF50 automatic blood cell analyzer, and the results were as follows: image 3 As shown, the white blood cells can be divided into four clear cell groups of lymphocytes, monocytes, neutrophils and eosinophils on the scatter diagram.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a white blood cell classification reagent, which comprises the following raw materials: an ionic surfactant, a pH value adjusting buffer, an alkali metal salt for adjusting osmotic pressure, and an alcohol capable of dissolving and accelerating. According to the present invention, with the white blood cell classification reagent, white blood cells can be divided into four cell clusters, and the boundary between each cell cluster is clear; compared to the traditional five-class hemolytic agent, the white blood cell classification reagent of the present invention has thefollowing advantages that the boundary between the monocyte clusters and the neutrophil cluster is clear, and the clustering property is strong; the white blood cell classification reagent has characteristics of strong low-temperature hemolysis ability and wide temperature range, and can rapidly dissolve red blood cells in a low-temperature environment, ensure the normal classification of white blood cells, and reduce the manufacturing cost of the instrument on temperature control; and the white blood cell classification reagent further has advantages of low raw material cost, environmental protection and no toxicity, and can classify white blood cells by requiring only one reagent so as to achieve advantages of convenience, time saving and reduced production cost.

Description

technical field [0001] The invention relates to the field of blood cell analysis, and mainly relates to a white blood cell classification reagent. Background technique [0002] In clinical practice, traditional blood routine examination uses manual microscopy to analyze parameters such as white blood cells, red blood cells, and platelets in the blood. However, manual operation is inefficient and has large errors, which is not suitable for large-scale inspection needs. The emergence of blood cell analyzers has changed the traditional blood analysis methods, making blood analysis gradually develop in the direction of automation and standardization. Among them, the classification and counting of white blood cells is an important part of clinical testing. Collecting blood samples from patients, classifying and counting white blood cells can provide evidence for clinical diagnosis and treatment, and play a very important role in disease diagnosis. [0003] So far, there are many...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N15/14
CPCG01N15/1434G01N2015/1006G01N2015/1486
Inventor 张勇亮郑剑通李春晖余晓尘
Owner SHENZHEN DYMIND BIOTECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products