White blood cell classification reagent

Abstract

The invention discloses a white blood cell classification reagent, which comprises the following raw materials: an ionic surfactant, a pH value adjusting buffer, an alkali metal salt for adjusting osmotic pressure, and an alcohol capable of dissolving and accelerating. According to the present invention, with the white blood cell classification reagent, white blood cells can be divided into four cell clusters, and the boundary between each cell cluster is clear; compared to the traditional five-class hemolytic agent, the white blood cell classification reagent of the present invention has thefollowing advantages that the boundary between the monocyte clusters and the neutrophil cluster is clear, and the clustering property is strong; the white blood cell classification reagent has characteristics of strong low-temperature hemolysis ability and wide temperature range, and can rapidly dissolve red blood cells in a low-temperature environment, ensure the normal classification of white blood cells, and reduce the manufacturing cost of the instrument on temperature control; and the white blood cell classification reagent further has advantages of low raw material cost, environmental protection and no toxicity, and can classify white blood cells by requiring only one reagent so as to achieve advantages of convenience, time saving and reduced production cost.

Description

technical field

[0001] The invention relates to the field of blood cell analysis, and mainly relates to a white blood cell classification reagent. Background technique

[0002] In clinical practice, traditional blood routine examination uses manual microscopy to analyze parameters such as white blood cells, red blood cells, and platelets in the blood. However, manual operation is inefficient and has large errors, which is not suitable for large-scale inspection needs. The emergence of blood cell analyzers has changed the traditional blood analysis methods, making blood analysis gradually develop in the direction of automation and standardization. Among them, the classification and counting of white blood cells is an important part of clinical testing. Collecting blood samples from patients, classifying and counting white blood cells can provide evidence for clinical diagnosis and treatment, and play a very important role in disease diagnosis.

[0003] So far, there are many...

Examples

Embodiment 1

[0046] A reagent for the five classifications of white blood cells, which consists of the following components:

[0047]

[0048] The pH of the reagent prepared according to the above formula is 4.5. The above reagents were tested on the blood sample A on the DF50 automatic blood cell analyzer, and the results were as follows: figure 1 As shown, the white blood cells can be divided into four clear cell groups of lymphocytes, monocytes, neutrophils and eosinophils on the scatter diagram.

Embodiment 2

[0050] A reagent for the five classifications of white blood cells, which consists of the following components:

[0051]

[0052]

[0053] The pH of the reagent prepared according to the above formula is 5.5. The above reagents were tested on blood sample B on the DF50 automatic blood cell analyzer, and the results were as follows: figure 2 As shown, the white blood cells can be divided into four clear cell groups of lymphocytes, monocytes, neutrophils and eosinophils on the scatter diagram.

Embodiment 3

[0055] A reagent for the five classifications of white blood cells, which consists of the following components:

[0056]

[0057] The pH of the reagent prepared according to the above formula is 4.2. The above reagents were tested on blood sample C on the DF50 automatic blood cell analyzer, and the results were as follows: image 3 As shown, the white blood cells can be divided into four clear cell groups of lymphocytes, monocytes, neutrophils and eosinophils on the scatter diagram.

technical field

[0001] The invention relates to the field of blood cell analysis, and mainly relates to a white blood cell classification reagent. Background technique

[0002] In clinical practice, traditional blood routine examination uses manual microscopy to analyze parameters such as white blood cells, red blood cells, and platelets in the blood. However, manual operation is inefficient and has large errors, which is not suitable for large-scale inspection needs. The emergence of blood cell analyzers has changed the traditional blood analysis methods, making blood analysis gradually develop in the direction of automation and standardization. Among them, the classification and counting of white blood cells is an important part of clinical testing. Collecting blood samples from patients, classifying and counting white blood cells can provide evidence for clinical diagnosis and treatment, and play a very important role in disease diagnosis.

[0003] So far, there are many...

Examples

Embodiment 1

[0046] A reagent for the five classifications of white blood cells, which consists of the following components:

[0047]

[0048] The pH of the reagent prepared according to the above formula is 4.5. The above reagents were tested on the blood sample A on the DF50 automatic blood cell analyzer, and the results were as follows: figure 1 As shown, the white blood cells can be divided into four clear cell groups of lymphocytes, monocytes, neutrophils and eosinophils on the scatter diagram.

Embodiment 2

[0050] A reagent for the five classifications of white blood cells, which consists of the following components:

[0051]

[0052]

[0053] The pH of the reagent prepared according to the above formula is 5.5. The above reagents were tested on blood sample B on the DF50 automatic blood cell analyzer, and the results were as follows: figure 2 As shown, the white blood cells can be divided into four clear cell groups of lymphocytes, monocytes, neutrophils and eosinophils on the scatter diagram.

Embodiment 3

[0055] A reagent for the five classifications of white blood cells, which consists of the following components:

[0056]

[0057] The pH of the reagent prepared according to the above formula is 4.2. The above reagents were tested on blood sample C on the DF50 automatic blood cell analyzer, and the results were as follows: image 3 As shown, the white blood cells can be divided into four clear cell groups of lymphocytes, monocytes, neutrophils and eosinophils on the scatter diagram.

Images