Synergistic application of farnesol on triazole fungicides in prevention and treatment of smut
A technology for farnesol and smut, applied in the direction of fungicides, applications, biocides, etc., can solve the problems of long retention period, pesticide environmental pollution, etc., and achieve the effect of improving bactericidal ability
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Embodiment 1
[0037] On the basis of the preliminary test, the farnesol and 10% difenoconazole microemulsion were prepared in proportion to a certain concentration gradient mother solution for use (farnesol: 0.05μM, 0.08μM, 0.1μM, 0.2μM, 0.3μM , 0.4μM, 0.5μM; 10% difenoconazole microemulsion (diluted): 1×10 5 Times, 5×10 4 Times, 3.34×10 4 times, 2×10 4 Times, 1.25×10 4 Times, 1×10 4 Times, 6.67×10 3 Times, 5×10 3 times). Pick a little sugarcane smut basidiospores from the plate and culture them overnight in 6mL YEPS culture medium as seed liquid, collect the bacteria by centrifugation the next day, and resuspend the bacteria with YEPS liquid medium (OD 600 0.2) for use. Draw 300 μL of mother solutions of different concentrations into 2 mL sterilized centrifuge tubes, add an equal amount of basidiospore suspension and mix well as a single use group; draw 250 μL of mother solutions of different concentrations of farnesol and 250 μL of azoxystrobin mother solutions and mix them in 5 m...
Embodiment 2
[0040] On the basis of the preliminary test, farnesol and 10% difenoconazole microemulsion were prepared in proportion to a certain concentration gradient mother solution for use (farnesol: 0.5 μM, 10% difenoconazole microemulsion: dilute 5000 times). Pick a little sugarcane smut basidiospores from the plate and culture overnight in 6mL YEPS culture solution as seed solution, transfer 500 μL to 50mL YEPS culture solution for expansion culture (to OD) the next day 600 0.2), draw 500 μL of different concentrations of mother solution into 5 mL sterilized centrifuge tubes, add an equal volume of bacterial solution and mix; then dilute 5000 times 10% difenoconazole microemulsion to 5 mL with 250 μL farnesol + 250 μL Add an equal volume of bacterial solution to a sterilized centrifuge tube and mix well; add 500 μL YEPS to a 5 mL sterilized centrifuge tube, add an equal volume of bacterial solution and mix well, and place in a shaker at 28°C and 200 rpm / min for 24 hours. Then use fr...
Embodiment 3
[0045] On the basis of the preliminary test, farnesol and 10% difenoconazole microemulsion were prepared in proportion to a mother solution with a certain concentration gradient for later use (farnesol: 0.08 μM, 10% difenoconazole microemulsion: dilute 2×10 4 times). Pick a little sugarcane smut basidiospores from the plate and culture overnight in 6mL YEPS culture solution as seed solution, transfer 500 μL to 50mL YEPS culture solution for expansion culture (to OD) the next day 600 0.2) for use. Control the concentration of farnesol to 0.08μM, and dilute the concentration of difenoconazole to 2×10 4 times, and then set a gradient dilution sequence to 0.5 times, 0.4 times, 0.25 times, 0.1 times, 0.05 times of the mother solution. Take the same 250 μL of farnesol and the same volume of different concentrations of difenoconazole and mix them in a centrifuge tube, and use 500 μL of farnesol and azoxystrobin mother solutions as a single use group; 500 μL of YEPS as a blank cont...
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