Application of taro leaf ethanol extract in improvement of immunity
A technology of ethanol extraction and immunity, applied in the field of medicine, to achieve the effect of comprehensive effect, expansion of application field and remarkable effect
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Embodiment 1
[0060] Embodiment 1, the improving immunity purposes of bean taro leaf ethanol extract, as Figure 1-Figure 11 As shown, the ethanol extract of bean taro leaf has a significant effect of improving immunity.
[0061] The specific preparation steps of the taro leaf ethanol extract are as follows:
[0062] (1) According to the solid-liquid ratio of 1g: 8ml, after mixing the taro leaves and 85% ethanol containing 0.1mol / L hydrochloric acid, the pulp is processed by a juice extractor. The obtained slurry was leached by ultrasonic (ultrasonic frequency of 40,000 Hz) for 240 min at 45° C., the extract was centrifuged at 4000 rpm for 30 min, and the extract was centrifuged to obtain a supernatant.
[0063] (2), replace the bean taro leaves in step (1) with the centrifuged filter residue obtained in step (1), repeat the extraction 2 times, merge the filtrate three times, and use the obtained supernatant in a rotary evaporator (the process parameters set by the rotary evaporator The t...
Embodiment 2
[0075] Embodiment 2, the influence of bean taro leaf ethanol extract on thymus index and spleen index of immunosuppressed mice;
[0076] The mice in Example 1 were sacrificed 30 days after gavage according to step 1, the thymus and spleen of the mice were removed and weighed, and the thymus index and spleen index of the mice were calculated.
[0077] The result is as figure 2 with image 3 As shown, compared with the blank control group, the thymus index and spleen index of the mice in the model group were significantly decreased. Compared with the model group, the thymus index and spleen index of the positive control group and each bean taro leaf ethanol extract treatment group tended to return to normal, and both the low-dose group and the high-dose group could significantly increase the thymus index and spleen index of the immunocompromised mice .
Embodiment 3
[0078] Embodiment 3, bean taro leaf ethanol extract is to the influence of immunosuppressed mouse peripheral blood routine;
[0079] In Example 1, the mice were gavaged for 30 days according to step 1, and the whole blood was collected by removing the eyeballs of the mice, and the eyeballs of one or both sides of the mice were removed with clean and sterile ophthalmic tweezers, and the blood dripped freely. In a 1.5ml centrifuge tube (or a commercially available anticoagulant tube) filled with anticoagulant, the blood and anticoagulant were continuously mixed during blood collection to prevent blood coagulation, and about 1ml of anticoagulant whole blood was collected from each animal. Mix the blood by inversion before going to the machine, and pay attention to check for clots. The total number of white blood cells and the number of lymphocytes were detected with a complete blood cell analyzer within 24 hours, and the operation on the machine was performed according to the ins...
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