Glycyrrhiza polysaccharide granules and preparation method thereof

A technology of licorice polysaccharides and granules, which is applied in the field of traditional Chinese medicine formula granule health food, can solve the problems of licorice polysaccharides in the field of research and development of health care products, etc., and achieve obvious health care functions, convenient use, and obvious effects of immunomodulatory activity

Inactive Publication Date: 2012-06-27
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to solve the problems in the prior art, the present invention provides a licorice polysaccharide granule and its preparation method, which solves the blank problem of licorice polysaccharide in the research and development field of health care products

Method used

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  • Glycyrrhiza polysaccharide granules and preparation method thereof
  • Glycyrrhiza polysaccharide granules and preparation method thereof

Examples

Experimental program
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preparation example Construction

[0021] Preparation of licorice polysaccharide

[0022] (1) Obtaining of licorice aseptic seedlings: select licorice seeds full of particles and dark green, stir and sterilize the seeds on a magnetic stirrer with 10% sodium hypochlorite, the rotating speed is 300r / min, and the temperature is 25°C. Bacteria time 30min, MS culture containing 3% sucrose, pH 5.9 before sterilization, inoculated in a 50ml Erlenmeyer flask containing MS solid medium, placed in a light incubator at 25°C, and cultivated for 30 days to obtain licorice sterile seedlings .

[0023] (2) Acquisition of licorice callus: the licorice callus was induced from the hypocotyls of the aseptic seedlings of licorice, and after several times of selection and subculture, the growth state was stable. The subculture medium of callus was MS medium, supplemented with 2,4-D (1 mg·L -1 ), NAA (1mg·L -1 ), 6-BA (0.2mg·L -1 ), 3% sucrose, pH 5.9 before sterilization, subculture cycle 28 days.

[0024] (3) Obtaining licori...

Embodiment 1

[0027] Take licorice suspension cells as raw material, take 2kg of cell powder, add 20 times the mass of licorice cell powder in water, soak for 12 hours, heat water bath for extraction, temperature 100°C, extract for 1 hour, repeat extraction twice, concentrate to a certain volume, add 4 times the volume Measure absolute ethanol, let it stand at 4°C for 12h, remove the supernatant, remove protein by sevage method, blow dry at 45°C for 24h, and grind to obtain licorice polysaccharide powder.

[0028] Take 100g of the above-mentioned licorice polysaccharide, 200g of hydroxypropyl methylcellulose, and 150g of lactose, mix well, pass through a 100-mesh sieve, add 20g of 1-5% ethyl cellulose ethanol solution, dry at 60°C for 5 hours, and then pass through a 20-mesh sieve Whole the grains to obtain the licorice polysaccharide granule preparation.

Embodiment 2

[0030] Take licorice suspended cells as raw material, take 2kg of cell powder, add 15 times the mass of licorice cell powder in water, soak for 5 hours, heat water bath for extraction, temperature 100°C, extract for 3 hours, repeat extraction once, concentrate to a certain volume, add 4 times the volume Measure absolute ethanol, let stand at 4°C for 12h, remove the supernatant, remove protein by sevage method, air-dry at 45°C for 24h, grind to obtain white licorice polysaccharide powder.

[0031] Take 100g of the above-mentioned licorice polysaccharide, 200g of ethyl cellulose, and 150g of mannitol, mix well, pass through a 100-mesh sieve, add 20g of 1-5% ethanol solution of ethyl cellulose, dry at 60°C for 5 hours, and then pass through a 20-mesh sieve Granules to obtain licorice polysaccharide granule preparations.

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Abstract

The invention discloses glycyrrhiza polysaccharide granules and a preparation method thereof; a tissue culture method is adopted to produce glycyrrhiza polysaccharide, and the glycyrrhiza polysaccharide granules can be prepared by the glycyrrhiza polysaccharide and other excipients. The glycyrrhiza polysaccharide granules comprise the components by parts: 100-150 parts of glycyrrhiza polysaccharide, 100-300 parts of sustained-release material, 100-200 parts of filler and 5-10 parts of adhesive. The glycyrrhiza polysaccharide is produced by being cultured by a fermentation tank; the developed glycyrrhiza polysaccharide granules are convenient to use and can be directly taken or taken with water; the produced glycyrrhiza polysaccharide has obvious immune dispensing activity and is capable of activating macrophage and tuberculosis (TB) lymphocyte, so that humoral immunity and cellular immunity can be enhanced, and the aims of improving the general immunity function and more effectively inhibiting tumours and acquired immunodeficiency syndrome (AIDS) can be achieved; furthermore, products developed by the polysaccharide have obvious health care function so as to have excellent develop space.

Description

technical field [0001] The invention relates to a health food and a preparation method thereof, in particular to a Chinese medicine formula granule health food. Background technique [0002] Glycyrrhiza plants are perennial herbaceous plants of the Fabaceae Papilionaceae family. The study of plant cell suspension culture is an important step towards industrial production of plant tissue culture. The liquid suspension culture of plant cells has the outstanding advantages of fast cell proliferation, can provide a large number of uniform cultures and can be cultivated on a large scale. Therefore, since the beginning of this century, the research on the production of medicinal active substances by plant cells, tissues and organs has made amazing progress. Nearly 1,000 kinds of plants have been studied on cell culture. It is estimated that 400 Tissue and cell cultures have been established in various plants and more than 600 metabolites have been isolated from them. As a tradit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/09
Inventor 高文远郭松波满淑丽刘辉王仁芳王娟
Owner TIANJIN UNIV
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