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Embryo sex interference preparation for sheep as well as preparation method and application thereof

An application method and preparation technology, which is applied in the field of embryo sex interference preparations for sheep, can solve the problems of high technical requirements for sperm thawing and artificial insemination, the inability to use large-scale flock breeding and production, and the difficulty of using small farms, etc., to achieve The effect of improving the chance of conception, low cost, and easy to use

Inactive Publication Date: 2018-08-28
陕西奥能生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] 1) High price, low benefit: The price of each sex-controlled semen for sheep is about 300 yuan, and the artificial insemination is one at a time, and the pregnancy rate is about 30%, so one ewe lamb (90% possibility) The cost is about 1,000 yuan, but the price of most ewe lambs (such as dairy goats) is about 500-800 yuan, so most people will not choose such products
[0006] 2) Low pregnancy rate: The number of sperm in each sexually controlled semen straw is about 2 million, while the normal semen straw contains 20 million sperm. The number of sperm is small, so the fertilization rate is low, making the pregnancy rate low reach about 30%
[0007] 3) Sperm thawing and artificial insemination require high technology, which is difficult for ordinary small farms to use
[0008] Therefore, this product cannot be used in the breeding and production of large-scale sheep flocks. In addition, there is no similar technology or product that can be used to increase the proportion of ewes in sheep
[0009] In 2010, the inventor developed a gender-disrupting preparation for cows, and applied for a national invention patent (CN102526701B), but this interference preparation is not suitable for sheep. It is extremely important to use gender disrupting agents

Method used

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  • Embryo sex interference preparation for sheep as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] An embryo sex disrupting preparation for sheep, the ingredients of the preparation and the mass parts of each ingredient can be as shown in the following ① to ⑤.

[0031] ①Vitamin B12 (VB12, C 63 h 88 CoN 14 o 14 P) 1 part, vitamin C (VC, C 6 h 8 o 6 ) 3 parts, 1 part of gentamicin sulfate, 2.2 parts of oxytocin and 1 part of glucose solution; ②Vitamin B12 (VB12, C 63 h 88 CoN 14 o 14 P) 3 parts, vitamin C (VC, C 6 h 8 o 6 ) 1 part, 2 parts of gentamicin sulfate, 0.8 parts of oxytocin and 2 parts of glucose solution; ③Vitamin B12 (VB12, C 63 h 88 CoN 14 o 14 P) 1.5 parts, vitamin C (VC, C 6 h 8 o 6 ) 2 parts, 1.5 parts of gentamicin sulfate, 1.5 parts of oxytocin and 1.5 parts of glucose solution; ④ Vitamin B12 (VB12, C 63 h 88 CoN 14 o 14 P) 2 parts, vitamin C (VC, C 6 h 8 o 6 ) 1.5 parts, gentamicin sulfate 1.5 parts, oxytocin 1.8 parts and glucose solution 1.5 parts; ⑤Vitamin B12 (VB12, C 63 h 88 CoN 14 o 14 P) 1.8 parts, vitamin C (VC, C...

Embodiment 2

[0034] A preparation method of an embryo sex disrupting preparation for sheep, the preparation method is as follows:

[0035] S1: Preparation of raw materials: Take vitamin B12, vitamin C, gentamicin sulfate, oxytocin and 5% glucose solution according to the ratio in Example 1;

[0036] S2: Add vitamin B12, vitamin C, gentamicin sulfate and 5% glucose solution into the container in sequence, stir for 5 minutes at 18°C, and finally add oxytocin and stir for 5 minutes to make sheep embryos gender disrupting agents;

[0037] S3: Packaging: 2ml glass ampoules are mechanically filled and sealed under aseptic conditions, and 10 bottles are packed in a cardboard box.

Embodiment 3

[0039] An application method of an embryo sex disrupting preparation for sheep, the application method includes the following three types:

[0040] (1) Conventional cervical artificial insemination: when inseminating ewes in estrus, first use an insemination device to inject 2 ml into the 1-3 cm of the cervix, then slowly withdraw the insemination device, and then follow the same steps Insemination can be completed. Infusion and insemination should be at the same location;

[0041] (2) Laparoscopic artificial insemination: mix 1 ml of this product with a sufficient amount of semen, and inject it into the uterine horns on both sides at one time;

[0042](3) In vitro fertilization of eggs and culture of embryos: Use 2 ml of this product, a tube of semen and embryo culture fluid to form a fertilization fluid, and put the collected unfertilized eggs into the fertilization fluid for in vitro fertilization.

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Abstract

The invention relates to an embryo sex interference preparation for sheep. The embryo sex interference preparation is prepared from the following components in parts by mass: 1-3 parts of vitamin B12,1-3 parts of vitamin C, 1-2 parts of gentamicin sulfate, 0.8-2.2 parts of oxytocin and 1-2 parts of glucose liquid. The preparation has the advantages that the activity of Y-chromosome sperms of thesheep can be obviously inhibited, capacitation of X-chromosome sperms can be promoted, the movement of the X-chromosome sperms can be promoted, the activity ability of the X-chromosome sperms can be enhanced, the survival time of the X-chromosome sperms can be prolonged, the combination probability of the X-chromosome sperms with eggs can be increased, and therefore the sex of embryos can be interfered and influenced, and finally the effect of improving the proportion of female lambs can be achieved.

Description

technical field [0001] The invention relates to the technical field of animal husbandry, in particular to an embryo sex-interfering preparation for sheep, a preparation method and an application thereof. Background technique [0002] Animal husbandry is already a major pillar industry of agriculture and agricultural industrialization, especially the sheep industry has become the second largest animal husbandry industry after the dairy industry. The breeding of sheep is the first job faced by all farms and runs through it all. Generally, one breeding ram can meet the breeding needs of about 100 ewes, that is to say, in most cases, the value of ewes is higher than that of rams, and people expect more ewes to be bred. The male and female lambs generally account for 50% of each gender, and sometimes the proportion of rams is greater than that of ewes, which greatly affects the economic benefits of the farm. Thereby people expect a kind of technology that can improve the ratio ...

Claims

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Application Information

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IPC IPC(8): A61K38/11A61P15/00A61P15/08A01K67/027A61K31/714A61K31/375A61K31/7036A61K31/7004A61K38/095
CPCA01K67/027A01K2227/102A01K2227/103A01K2267/02A61K31/375A61K31/7004A61K31/7036A61K31/714A61P15/00A61P15/08A61K38/095A61K2300/00
Inventor 杨永祥马拴林李拴军
Owner 陕西奥能生物科技有限公司
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