Embryo sex interference preparation for sheep as well as preparation method and application thereof
An application method and preparation technology, which is applied in the field of embryo sex interference preparations for sheep, can solve the problems of high technical requirements for sperm thawing and artificial insemination, the inability to use large-scale flock breeding and production, and the difficulty of using small farms, etc., to achieve The effect of improving the chance of conception, low cost, and easy to use
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Embodiment 1
[0030] An embryo sex disrupting preparation for sheep, the ingredients of the preparation and the mass parts of each ingredient can be as shown in the following ① to ⑤.
[0031] ①Vitamin B12 (VB12, C 63 h 88 CoN 14 o 14 P) 1 part, vitamin C (VC, C 6 h 8 o 6 ) 3 parts, 1 part of gentamicin sulfate, 2.2 parts of oxytocin and 1 part of glucose solution; ②Vitamin B12 (VB12, C 63 h 88 CoN 14 o 14 P) 3 parts, vitamin C (VC, C 6 h 8 o 6 ) 1 part, 2 parts of gentamicin sulfate, 0.8 parts of oxytocin and 2 parts of glucose solution; ③Vitamin B12 (VB12, C 63 h 88 CoN 14 o 14 P) 1.5 parts, vitamin C (VC, C 6 h 8 o 6 ) 2 parts, 1.5 parts of gentamicin sulfate, 1.5 parts of oxytocin and 1.5 parts of glucose solution; ④ Vitamin B12 (VB12, C 63 h 88 CoN 14 o 14 P) 2 parts, vitamin C (VC, C 6 h 8 o 6 ) 1.5 parts, gentamicin sulfate 1.5 parts, oxytocin 1.8 parts and glucose solution 1.5 parts; ⑤Vitamin B12 (VB12, C 63 h 88 CoN 14 o 14 P) 1.8 parts, vitamin C (VC, C...
Embodiment 2
[0034] A preparation method of an embryo sex disrupting preparation for sheep, the preparation method is as follows:
[0035] S1: Preparation of raw materials: Take vitamin B12, vitamin C, gentamicin sulfate, oxytocin and 5% glucose solution according to the ratio in Example 1;
[0036] S2: Add vitamin B12, vitamin C, gentamicin sulfate and 5% glucose solution into the container in sequence, stir for 5 minutes at 18°C, and finally add oxytocin and stir for 5 minutes to make sheep embryos gender disrupting agents;
[0037] S3: Packaging: 2ml glass ampoules are mechanically filled and sealed under aseptic conditions, and 10 bottles are packed in a cardboard box.
Embodiment 3
[0039] An application method of an embryo sex disrupting preparation for sheep, the application method includes the following three types:
[0040] (1) Conventional cervical artificial insemination: when inseminating ewes in estrus, first use an insemination device to inject 2 ml into the 1-3 cm of the cervix, then slowly withdraw the insemination device, and then follow the same steps Insemination can be completed. Infusion and insemination should be at the same location;
[0041] (2) Laparoscopic artificial insemination: mix 1 ml of this product with a sufficient amount of semen, and inject it into the uterine horns on both sides at one time;
[0042](3) In vitro fertilization of eggs and culture of embryos: Use 2 ml of this product, a tube of semen and embryo culture fluid to form a fertilization fluid, and put the collected unfertilized eggs into the fertilization fluid for in vitro fertilization.
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