Pancreatic duct adenocarcinoma marker and application thereof

A pancreatic ductal adenocarcinoma and biomarker technology, applied in the field of tumor diagnosis and molecular targeted therapy, can solve the problems of only 15%, less than 20% survival rate, easy recurrence and metastasis, etc.

Inactive Publication Date: 2018-08-28
THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the high degree of malignancy and rapid progression of pancreatic cancer, its prognosis is extremely poor, and the overall 5-year survival rate is less than 5%.
Although radical surgical resection can improve the prognosis and increase the long-term survival rate, due to the hidden onset, rapid progression, and easy recurrence and metastasis of pancreatic cancer, the radical surgical resection rate is only 15%, and the 5-year survival rate after surgery is insufficient. 20%
However, the expression and biological function of LINC01133 in pancreatic cancer have not been reported; after searching, there is no patent application for LINC001133 gene as a biological tumor marker in pancreatic ductal adenocarcinoma

Method used

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  • Pancreatic duct adenocarcinoma marker and application thereof
  • Pancreatic duct adenocarcinoma marker and application thereof
  • Pancreatic duct adenocarcinoma marker and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1: The relationship between the LINC01133 gene and pancreatic ductal adenocarcinoma was mined through the database, and the difference in the expression level of the LINC01133 gene in pancreatic ductal adenocarcinoma patient specimens was compared between cancer and paracancerous.

[0019] The Cancer Genome Atlas (TCGA) database contains multiple levels of data information for various tumor types, including: miRNA, mRNA, protein profile, mutation profile, clinical diagnosis information, etc. These data provide a rich resource for tumor data analysis. Therefore, we first analyzed the correlation between LINC01133 gene and cancer with the help of TCGA database (https: / / tcga-data.nci.nih.gov / tcga / ).

[0020] (1) Three sets of gene chip data (GSE15471, GSE16515, GSE32676) mined in the GEO database (https: / / www.ncbi.nlm.nih.gov / gds / ) were selected for analysis. Among them, the gene LINC01133 was differentially expressed in the cancer and adjacent tumors in the three...

Embodiment 2

[0023] Example 2: Combined with the clinical samples of 134 patients with pancreatic ductal adenocarcinoma in the First Affiliated Hospital of Sun Yat-sen University, RT-PCR was performed to detect the expression of LINC01133 gene and different samples of pancreatic ductal adenocarcinoma patients.

[0024] The detection process is as follows:

[0025] 1. Extract total RNA from pancreatic ductal adenocarcinoma tumors and matched normal tissues,

[0026] (1) About 20mg of fresh or frozen tissue at -80°C, add 1ml Trizol and grind on ice. You can cut the tissue with scissors first. To prevent overflow, generally add 400ul Trizol first, grind it fully, and then make up to 1ml. Place at room temperature for 5-10min.

[0027] (2) Add chloroform according to the volume ratio of Trizol and chloroform at a ratio of 5:1, vortex for 30 s, place at room temperature for 5 min, and centrifuge at 12000 g for 15 min at 4°C.

[0028] (3) Aspirate about 400ul of the supernatant layer, try to b...

Embodiment 3

[0059] Example 3: Detection of the influence of LINC01133 on cell growth in pancreatic ductal adenocarcinoma cells SW1990 and BXPC3.

[0060] First, the LINC01133 gene was knocked down in SW1990 and BXPC3 cells using siRNA gene editing technology. Control cells and LINC01133 knockdown cells were cultured in DMEM / F12 complete medium (DMEM+10% fetal bovine serum+100-U / ml penicillin / streptomycin) at 37°C in a 5% CO2 incubator. After the cells grow on the wall, take the cells in the logarithmic growth phase, extract the total RNA, and test the knockdown efficiency of the LINC0133 gene. The results are shown in Figure 4

[0061] Then, cells were trypsinized and counted at 2x10 per well 3 Cells were seeded in a 96-well plate and cultured in a 37°C incubator. At 12h, 24h, 36h, and 48h of cell culture, the cells were digested with trypsin and counted. It can be seen from the results that the growth rate of SW1990 and BXPC3 cells was significantly reduced after knocking out LINC01...

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Abstract

The invention discloses a pancreatic duct adenocarcinoma related tumor marker LINC01133, and application of a kit for detecting expression level of LINC01133 in the detection and diagnosis of pancreatic duct adenocarcinoma or patient prognostic evaluation. The expression level of LINC01133 is closely related to the clinical pathological parameters of pancreatic duct adenocarcinoma and the patientprognosis, and can be adopted as a specific tumor marker in pancreatic duct adenocarcinoma detection and a prognostic indicator, and thus is of an important clinical value.

Description

technical field [0001] The invention belongs to the field of tumor diagnosis and molecular targeted therapy, and relates to a tumor marker related to pancreatic ductal adenocarcinoma and its application. Background technique [0002] In recent years, the incidence of pancreatic ductal adenocarcinoma (Pancreatic Duct Adenocarcinoma) has been increasing year by year worldwide. Due to the high degree of malignancy and rapid progression of pancreatic cancer, its prognosis is extremely poor, and the overall 5-year survival rate is less than 5%. Although radical surgical resection can improve the prognosis and increase the long-term survival rate, due to the hidden onset, rapid progression, and easy recurrence and metastasis of pancreatic cancer, the radical surgical resection rate is only 15%, and the 5-year survival rate after surgery is insufficient. 20%. In addition, pancreatic cancer still lacks specific chemotherapy drugs and targeted therapy drugs, which are not highly se...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886A61K31/7088A61P35/00
CPCA61K31/7088A61P35/00C12Q1/6886C12Q2600/118C12Q2600/158C12Q2600/178
Inventor 殷晓煜赵蔚黄晨松
Owner THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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