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Inactivated clostridium butyricum injection

A technology for inactivating Clostridium butyricum and live Clostridium butyricum, which is used in the field of antibacterial and antiviral injections

Active Publication Date: 2018-09-04
WEIFANG HUAYING BIOTECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no research report on the use of inactivated Clostridium butyricum as an injection for disease prevention and treatment at home and abroad

Method used

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  • Inactivated clostridium butyricum injection
  • Inactivated clostridium butyricum injection
  • Inactivated clostridium butyricum injection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Inoculate Clostridium butyricum (Latin name: Clostridium butyricum, purchased from China Industrial Microorganism Culture Collection Management Center, preservation number: CICC 20036) in MRS broth medium, anaerobically ferment at 37°C for 36-48 hours, and then 3000 Centrifuge for 5 minutes, remove the supernatant culture solution and retain the precipitate, add sterile normal saline to wash the precipitate, centrifuge for 5 minutes, repeat the washing 3 times, add sterile normal saline, mix with the precipitate, and make a suspension. Take a certain amount of Clostridium butyricum normal saline suspension, and carry out the bacterial count through the THOMA bacterial counting plate, so that each ml suspension contains about 10 9 Clostridium butyricum. The prepared Clostridium butyricum physiological saline suspension was inactivated at 121° C. under a pressure of 0.12 MPa for 15 minutes to obtain an inactivated Clostridium butyricum injection. Live Clostridium butyric...

Embodiment 2

[0019] The prevention and treatment effect of the inactivated Clostridium butyricum injection prepared in Example 1 on Salmonella-infected mice was tested. The clean-grade Kunming mice with a body weight of 18-22 g were divided into 3 groups, namely the normal control group, the Salmonella group and the inactivated Clostridium butyricum injection group, with 30 mice in each group, half male and half male. The mice in the normal control group and the Salmonella group were injected with sterile saline into the tail vein, and the mice in the inactivated Clostridium butyricum injection group were injected with the inactivated Clostridium butyricum suspension in the tail vein, and the administration volume of mice in each group was 0.1 mL / 10g. At intervals of 24 hours after administration, the mice in the Salmonella group and the inactivated Clostridium butyricum injection group were injected with Salmonella enteritidis (China Center for Veterinary Microbiological Culture Collectio...

Embodiment 3

[0024] The effect of the inactivated Clostridium butyricum injection prepared in Example 1 on the number of deaths of mice infected with influenza virus PR8 strain was tested. In the experiment, 18-22g clean-grade Kunming mice were selected and divided into normal control group, PR8 strain model group (namely, influenza virus PR8 strain infected mouse model group) and inactivated Clostridium butyricum injection group. There were 30 mice in each group, half male and half male. The mice in the normal control group and the PR8 strain model group were injected with sterile saline into the tail vein, and the mice in the inactivated Clostridium butyricum injection group were injected with the inactivated Clostridium butyricum suspension in the tail vein, and the administration volume of each group was 0.1 mL / 10g. The mice in each group were administered at an interval of 24 hours after administration once. Except for the normal control group, the mice in other groups were inoculate...

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Abstract

The invention relates to an antibacterial and anti-virus injection and belongs to the field of biomedicines. A main component of the medicine is inactivated clostridium butyricum; the injection is prepared by inactivating live clostridium butyricum; the inactivated clostridium butyricum has antibacterial and anti-virus effects after being injected and can be used for prevention, treatment or adjuvant treatment of various bacterium diseases and virus diseases of human or animals, so that the injection has a wide application prospect.

Description

technical field [0001] The invention belongs to the field of biomedicine, in particular to an antibacterial and antiviral injection. Background technique [0002] Clostridium butyricum, also known as Clostridium butyricum, is an anaerobic Gram-positive bacillus isolated from the intestinal tract of healthy humans and animals. Current studies have found that Clostridium butyricum has good probiotic properties such as maintaining the balance of intestinal flora, enhancing the body's immune function, generating nutrients, and preventing enteritis. At present, live Clostridium butyricum preparations are orally used in the prevention and treatment of enteritis in human medicine, and live Clostridium butyricum is mostly used as feed additives for the health care of the digestive tract of livestock and poultry in the livestock and poultry industry. Someone isolated 2 strains of Clostridium butyricum from the feces of adults and young children, and confirmed that these 2 strains of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/742A61K9/10A61P31/04A61P31/12
CPCA61K9/0019A61K9/10A61K35/742A61P31/04A61P31/12
Inventor 张勇石有斐
Owner WEIFANG HUAYING BIOTECH CO LTD
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