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Method for extracting hematopoietic stem cells in placenta by using multifunctional placenta tissue processing device

A hematopoietic stem cell and processing device technology, applied in the field of placental tissue processing, can solve the problems of less interstitial cells, easy pollution, and labor time-consuming, etc., and achieve the effect of efficient cleaning and cell recovery

Active Publication Date: 2021-06-29
天晴干细胞股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problems that the placental tissue is easy to be polluted when the placental tissue is opened and processed during the cleaning and separation of conventional placenta cells. Methods of hematopoietic stem cells in the placenta

Method used

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  • Method for extracting hematopoietic stem cells in placenta by using multifunctional placenta tissue processing device
  • Method for extracting hematopoietic stem cells in placenta by using multifunctional placenta tissue processing device
  • Method for extracting hematopoietic stem cells in placenta by using multifunctional placenta tissue processing device

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specific Embodiment approach 1

[0042] Embodiment 1: This embodiment is a method for extracting hematopoietic stem cells in the placenta using a multi-functional placental tissue processing device, which is specifically completed according to the following steps:

[0043] 1. Clean the placenta:

[0044] ①. The lower end of the processor cylinder 8 of the placenta processing device is threadedly connected with the upper end of the conical treatment liquid receiver 10 of the cleaning liquid receiver, and the aseptically collected placenta is put into the processor cylinder 8, and the sterile physiological The saline is injected into the processor cylinder 8 through the closed silica gel cap 5, and the processor cylinder 8 is sealed; the volume ratio of the volume of the sterile saline to the processor cylinder 8 is 1:(16~40); the placenta The volume ratio of the volume to the processor cylinder 8 is 1:(10~20);

[0045] ②. Place the connecting pipe 6 and the cleaning fluid guide pipe 12 on the arc-shaped parts...

specific Embodiment approach 2

[0069] Embodiment 2: The difference between this embodiment and Embodiment 1 is: the diameter of the arc portion of the first belt 1 is 20 cm, and the diameter of the arc portion of the second belt 3 is 20 cm. Others are the same as the first embodiment.

specific Embodiment approach 3

[0070] Embodiment 3: The difference between this embodiment and Embodiment 1 or Embodiment 2 is that the diameter of the platform of the electromagnetic stirrer 2 with a platform is 20 cm. Others are the same as those of the specific embodiment one or two.

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Abstract

The invention discloses a method for extracting hematopoietic stem cells in the placenta by using a multifunctional placenta tissue processing device, which relates to the field of biotechnology, and specifically relates to a method for processing placenta tissue. The purpose of the present invention is to solve the problems that placental tissue is easy to be polluted when the placental tissue is opened and treated in the conventional placental cell cleaning and separation process, the process is labor-intensive, the process is incomplete, and the interstitial cells are less obtained. Placental tissue processing and processing methods: 1. Wash the placenta; 2. Break the placenta; 3. Collect placental hematopoietic stem cells, that is, complete the tissue processing of the placenta before separating stem cells. The invention is mainly used for tissue processing of placenta.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for processing placental tissue. Background technique [0002] After the fertilized egg is implanted with the endometrium of the mother, it obtains nutrients and metabolic waste through the placenta during the process of gradually developing into an individual. The placenta is a delicate structure, which is an important place for protection and exchange of material and energy between the mother and the baby during the growth of mammals. A full-term placenta has a diameter of about 160-200mm, a thickness of about 20-25mm, and a weight of 450-650 grams. Its structure includes amnion, phyllodes chorion, and decidua basalis. In the chorion, there are a large number of protruding villi leaflets composed of fetal veins and arteries, which greatly increases its specific surface area to about 14 square meters, greatly increasing the material exchange area between the fetus and the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0789C12M1/42C12M1/40C12M1/33C12M1/02C12M1/00
CPCC12M47/04C12N5/0647C12N2509/00C12N2509/10
Inventor 张怡聂艳波刘艳青
Owner 天晴干细胞股份有限公司
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