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Horseradish tree zymolyte as well as preparation method and application thereof

A technology of moringa enzyme and enzymatic hydrolyzate, which is applied in the field of preparation of moringa enzymatic hydrolyzate and moringa enzymatic hydrolyzate, can solve the problems of lagging development of moringa oleifera, poor efficacy, low absorption efficiency of moringa oleifera leaves, etc., and achieve Low production cost, high oral bioavailability, and reduced post-processing effects

Active Publication Date: 2018-09-04
成都金希生态农业开发股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the development of Moringa products in my country is relatively lagging behind. The functional foods on the market are mainly rough processed directly by crushing Moringa leaves. The absorption efficiency of Moringa leaves is low and the efficacy is not good.
Moreover, most of the current research on Moringa leaves is to extract flavonoids, and the research on polysaccharides, alkaloids, water-soluble proteins and other components and their active components is rarely seen.

Method used

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  • Horseradish tree zymolyte as well as preparation method and application thereof
  • Horseradish tree zymolyte as well as preparation method and application thereof

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preparation example Construction

[0027] The invention provides a kind of preparation method of Moringa oleifera hydrolyzate, comprises the following steps:

[0028] a. Mix 100 parts by weight of Moringa raw material with 800 to 1200 parts by weight of water, add 1 to 8 parts of carbohydrase to the mixture, and perform constant temperature enzymolysis at 30 to 65°C for 0.5 to 12 hours to obtain carbohydrate hydrolyzate;

[0029] b. Adjust the pH value of the sugar hydrolyzate to 8-9, add 1-8 parts by weight of alkaline protease to the sugar hydrolyzate, and perform constant temperature enzymolysis at 30-65°C for 0.5-12 hours to obtain primary proteolyzate;

[0030] c. Add 1-8 parts by weight of neutral protease to the primary proteolyzate obtained in step b, and perform constant temperature enzymolysis at 30-65° C. for 0.5-12 hours to obtain a final proteolyzate;

[0031] d. Inactivate the enzyme in the final protein hydrolyzate described in step c, and obtain Moringa oleifera hydrolyzate after filtering and ...

Embodiment 1

[0052] Preparation of Moringa oleifera leaf enzymatic hydrolyzate from Yunnan dried Moringa oleifera leaves

[0053] Crush 100 grams of clean Moringa oleifera leaves from Yunnan, add 1000 ml of deionized water at a constant temperature of 55°C, adjust the pH value to 5, add 2 grams of carbohydrase, and react for 3 hours; then adjust the pH value with sodium hydroxide solution at the same temperature To 8, add 2 grams of alkaline protease to react for 2 hours under constant temperature stirring; then add 2 grams of neutral protease to react for 2 hours under the same temperature conditions; raise the temperature of the obtained enzymatic hydrolyzate to 90 ° C, keep the temperature for half an hour, so that the enzyme loses Live, filter. The filtrate was concentrated under reduced pressure at a vacuum degree of 0.08 Mpa and a temperature of 50° C., and finally freeze-dried to obtain solid Moringa leaf enzymatic hydrolyzate dry powder with a yield of 62.1%.

[0054] According to...

Embodiment 2

[0059] Preparation of dry powder of Moringa oleifera leaf enzyme hydrolyzate from Sichuan moringa oleifera leaves

[0060] Add 100 grams of clean dried and crushed leaves of Moringa oleifera from Sichuan, add 800 ml of deionized water at a constant temperature of 35°C, adjust the pH value to 5, add 7 grams of carbohydrase, and react for 4 hours; then adjust the pH value to 8 at the same temperature , add 8 grams of alkaline protease and react for 5 hours under constant temperature stirring; then add 8 grams of neutral protease and react for 5 hours under the same temperature conditions; then raise the temperature to 80°C, react at constant temperature for 40 minutes to inactivate the enzyme, and filter. The filtrate was concentrated under reduced pressure at a vacuum degree of 0.10 MPa and a temperature of 40° C., and finally freeze-dried to obtain solid Moringa oleifera leaf enzymatic hydrolyzate dry powder with a yield of 48.5%.

[0061] According to the measurement, in the ...

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Abstract

The invention discloses horseradish tree zymolyte as well as a preparation method and application thereof and relates to the technical field of biological extraction. According to the horseradish treezymolyte disclosed by the invention, a horseradish tree raw material is resolved by carbohydrase at first, horseradish tree cell walls are broken, and other matters in the cells are released; then protease is used for degrading plant protein in the horseradish tree cells into small-molecular polypeptide; zymolyte obtained by degradation can be directly applied to drying after enzyme deactivationand filtering to obtain horseradish tree zymolyte; thus, a preparation technology is simple and convenient, and production cost is low. The prepared horseradish tree zymolyte can extract protein and polysaccharide in the horseradish trees to the maximum extent, can fully gather biological active small molecules, is easy to digest and absorb in the human body and has high bioavailability and a goodanti-fatigue effect.

Description

technical field [0001] The invention relates to the technical field of biological extraction, in particular to a method for preparing a moringa enzymatic hydrolyzate, the prepared moringa enzymatic hydrolyzate and its application. Background technique [0002] Moringa is a perennial tropical and subtropical deciduous tree belonging to the genus Moringa of the family Moringa. Triangle), known as Drumstick tree (Drumstick tree), because its root has a spicy taste, it is called Horseradish tree (Horseradish tree); because its seeds can extract oil, it is called Ben oil tree (Benzoil tree) . There are about 14 varieties of Moringa trees in the world, which can grow naturally outdoors in tropical or subtropical climates with an altitude of 0-2000 meters and an annual rainfall of 800-3000 mm. The soil conditions are relatively wide, and the sandy pH value is 5 It is most suitable in the soil of -9. The height of the tree is 10-12 meters, the trunk is erect, and the diameter can...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C12P19/14C12P19/04A23L33/00A61K36/185A61P39/00
CPCA23V2002/00A61K36/185A23L33/00A61P39/00C12P19/04C12P19/14C12P21/06A23V2200/30
Inventor 史宗洁张思良张涛朱筱莉周玮黄萍叶仁礼张军郭黄勋张晓凤
Owner 成都金希生态农业开发股份有限公司
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