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A kind of corn oligopeptide and its industrial production method

A corn oligopeptide, production method technology, applied in the preparation method of peptide, chemical instrument and method, peptide and other directions, can solve the problem of undetectable functional peptide segment, etc., to avoid loss, wide application prospect, broaden application range effect

Active Publication Date: 2021-03-19
CHINA NAT RES INST OF FOOD & FERMENTATION IND CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the existing corn oligopeptide products, the above-mentioned functional peptides are basically not detected

Method used

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  • A kind of corn oligopeptide and its industrial production method
  • A kind of corn oligopeptide and its industrial production method
  • A kind of corn oligopeptide and its industrial production method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] 1. Preparation of corn oligopeptides:

[0064] 1) Add 500g of corn gluten powder into the reaction tank, adjust the slurry with 5L of deionized water, add NaOH to adjust the pH value to 9.0, raise the temperature to 50°C, add 4.5g of alkaline protease, and then add neutral protease after 30min of enzymatic hydrolysis 3.6g, continue to enzymatically hydrolyze for 3.5h, heat up to 100°C to inactivate the enzyme, and maintain for 30min.

[0065] 2) Centrifuge the enzymolysis solution with a desktop centrifuge, collect the supernatant, and then filter it with a ceramic membrane with a pore size of 200 nm to further clarify the enzymolysis solution.

[0066] 3) Add 20 g of food-grade woody powdered activated carbon (100% fineness and pass through a 200 mesh sieve) to the enzymolysis solution, absorb at 50°C for about 30 minutes, and then remove the activated carbon.

[0067] 4) Pass the enzymatic hydrolysis solution adsorbed by activated carbon through the pre-balanced hydr...

Embodiment 2

[0076] 1. Preparation of corn oligopeptides:

[0077] 1) Add 500g of corn gluten powder into the reaction tank, adjust the slurry with 5L of deionized water, add NaOH to adjust the pH value to 9.0, raise the temperature to 50°C, add 4.5g of alkaline protease, and then add neutral protease after 30min of enzymatic hydrolysis 3.6g, continue to enzymatically hydrolyze for 3.5hr, heat up to 100°C to inactivate the enzyme, and maintain for 30min.

[0078] 2) Centrifuge the enzymolysis solution with a desktop centrifuge, collect the supernatant, and then filter it with a ceramic membrane with a pore size of 200 nm to further clarify the enzymolysis solution.

[0079] 3) Add 100 g of food-grade woody powdery activated carbon (fineness 100% through a 200-mesh sieve) to the enzymolysis solution, absorb at 50° C. for about 12 minutes, and then remove the activated carbon.

[0080] 4) Pass the enzymatic hydrolysis solution adsorbed by activated carbon through the pre-balanced hydrogen-typ...

Embodiment 3

[0087] 1. Preparation of corn oligopeptides:

[0088] 1) Add 500g of corn gluten powder into the reaction tank, adjust the slurry with 5L of deionized water, add NaOH to adjust the pH value to 9.0, raise the temperature to 50°C, add 4.5g of alkaline protease, and then add neutral protease after 30min of enzymatic hydrolysis 3.6g, continue to enzymatically hydrolyze for 3.5hr, heat up to 100°C to inactivate the enzyme, and maintain for 30min.

[0089] 2) Centrifuge the enzymolysis solution with a desktop centrifuge, collect the supernatant, and then filter it with a ceramic membrane with a pore size of 200 nm to further clarify the enzymolysis solution.

[0090] 3) Add 50 g of food-grade woody powdered activated carbon (fineness 100% through a 200 mesh sieve) to the enzymolysis solution, absorb at 50°C for about 30 minutes, and then remove the activated carbon.

[0091] 4) Pass the enzymatic hydrolysis solution adsorbed by activated carbon through the pre-balanced hydrogen-typ...

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Abstract

The invention provides a corn oligo-peptide and an industrial production method thereof, wherein the corn oligo-peptide is at least prepared from peptides pEL, VL and LL. The industrial production method of the corn oligo-peptide comprises the following steps of using prolease for implementing enzymolysis on corn gluten meal so as to obtain enzymolysis liquid; sequentially performing centrifugation, filtering, active carbon adsorption, cation exchange resin purification and anion exchange resin purification treatment on the enzymolysis solution. By using the industrial production method provided by the invention, the three functional peptides including the pEL with various physiological functions such as liver protection, inflammation resistance and anti-depression, and the VL and the LL used as the good branch chain amino acid supplementing agent can be remained.

Description

technical field [0001] The invention relates to a corn oligopeptide and its industrial production method, in particular to a corn oligopeptide with pyroglutamyl leucine, valyl leucine and leucyl leucine peptide segments and its Industrial production process. Background technique [0002] Corn is one of the three major food crops in the world, and it is also a traditional crop in my country. One of the main uses of corn is to produce corn starch, and the main by-product in the process of producing corn starch by wet milling is corn gluten meal (Corn Gluten Meal, CGM), also known as corn yellow powder. Corn gluten powder generally contains about 60% protein, and the main components are zein, gluten and globulin. Corn gluten powder contains a high proportion of hydrophobic amino acids such as leucine, isoleucine, valine, and alanine, and proline, glutamic acid, etc., but the content of essential amino acids such as lysine and tryptophan Low, this unique amino acid compositio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K1/18
CPCC07K1/14C07K1/18C07K1/34C07K1/36C12P21/06
Inventor 蔡木易谷瑞增鲁军张海欣潘兴昌董哲马勇徐亚光马永庆陈亮陆路刘文颖魏颖刘艳曹珂璐王憬李国明周明王雨辰王雨晴凌空毕园崔欣悦秦修远
Owner CHINA NAT RES INST OF FOOD & FERMENTATION IND CO LTD
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