Method of preparing polypeptide through enzymolysis of proteins in multi-algae
A proteolysis and pepsin technology, which is applied in the field of polyalgae proteolysis to prepare polypeptides, can solve the problems of affecting absorption, indigestion, affecting the biological activity of Euglena, and achieves good surface morphology and uniformity, and improves the effect of conversion rate.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0029] 1) Pretreatment: Add 700g 1,2-dimethoxyethane and 0.8L water to 300g Chlorella, crush and homogenize at 15°C and 120MPa, let stand, discard the upper organic phase,
[0030] 2) Homogenization: Add 100 g of spirulina and 2 g of euglena to the chlorella treated in step 1), add 1.6 L of water, crush and homogenize at 15° C. and 120 MPa to obtain a homogeneous liquid;
[0031] 3) Enzymolysis: Add a mixed enzyme solution consisting of bromelain and pepsin at a concentration of 200IU / mL to the treated medium in step 2), and perform enzymolysis at 36°C and pH=4 to obtain an enzymolysis solution; pineapple The activity ratio of protease and pepsin is 2:1;
[0032] 4) Enzyme inactivation: heat the enzymatic hydrolysis solution to 70°C to inactivate the enzyme, after cooling to room temperature, centrifuge the enzymatic hydrolysis solution at 4000rpm, and take the supernatant;
[0033] 5) Ultrafiltration: the supernatant is filtered through an ultrafiltration centrifuge tube to ...
Embodiment 2
[0037] 1) Pretreatment: Add 700g 1,2-dimethoxyethane and 0.8L water to 300g Chlorella, crush and homogenize at 15°C and 120MPa, let stand, discard the upper organic phase,
[0038] 2) Homogenization: add 120 g of spirulina and 3 g of euglena to the chlorella treated in step 1), add 1.7 L of water, break and homogenize at 15° C. and 120 MPa;
[0039] 3) Enzymolysis: Add a mixed enzyme solution composed of bromelain and pepsin at a concentration of 200IU / mL to the homogeneous solution treated in step 2), and perform enzymolysis at 36°C and pH=4 to obtain enzymolysis solution; the activity ratio of bromelain and pepsin is 3:1;
[0040] 4) Enzyme inactivation: heat the enzymatic hydrolysis solution to 70°C to inactivate the enzyme, after cooling to room temperature, centrifuge the enzymatic hydrolysis solution at 4000rpm, and take the supernatant;
[0041] 5) Ultrafiltration: the supernatant is filtered through an ultrafiltration centrifuge tube to filter polypeptide molecules wi...
Embodiment 3
[0044] 1) Pretreatment: Add 700g 1,2-dimethoxyethane and 0.8L water to 300g Chlorella, crush and homogenize at 15°C and 120MPa, let stand, discard the upper organic phase,
[0045] 2) Homogenization: add 130 g of spirulina and 4 g of euglena to the chlorella treated in step 1), add 1.8 L of water, break and homogenize at 15° C. and 120 MPa;
[0046] 3) Enzymolysis: Add a mixed enzyme solution composed of bromelain and pepsin at a concentration of 220IU / mL to the homogeneous solution treated in step 2), and perform enzymolysis at 36°C and pH=4 to obtain enzymolysis solution; the activity ratio of bromelain and pepsin is 1:1;
[0047] 4) Enzyme inactivation: heat the enzymatic hydrolysis solution to 70°C to inactivate the enzyme, after cooling to room temperature, centrifuge the enzymatic hydrolysis solution at 4000rpm, and take the supernatant;
[0048] 5) Ultrafiltration: the supernatant is filtered through an ultrafiltration centrifuge tube to filter polypeptide molecules wi...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More