Platelet antibody detection kit based on solid phase agglutination method and detection method and application thereof

Abstract

The invention relates to a platelet antibody detection kit based on solid phase agglutination method and detection method and application thereof. The kit comprises a box body, a fixed sponge arrangedin the box body, a low ion strength solution bottle, an anti-human globulin reagent bottle, a concentrated detergent bottle, a platelet antibody positive control reagent bottle, a platelet antibody negative control reagent bottle, a reaction plate and a sealing plate film. The reaction plate is packaged, the mouse anti-human platelet monoclonal antibody is coated in the reaction plate, a gap is reserved between the fixed sponge and the vertical surface of the box body, the width of the gap is matched with the width of the reaction plate so as to meet the side of the fixed sponge abutting tightly against the box body, the low ion strength solution bottle, the anti-human globulin reagent bottle, the concentrated washing liquid bottle, the platelet antibody positive control reagent bottle and the platelet antibody negative control reagent bottle are respectively fixed in the fixed hole preset by the fixed sponge. The platelet antibody detection kit based on solid phase agglutination method can meet repeated detection in the application and can be seen on a reaction plate for a plurality of tests, and the platelet antibody detection kit based on solid phase agglutination method has the advantages that the contrast is clear, the layout is scientific, the transportation and storage and the carrying are facilitated, and the detection process is convenient and rapid, the experiment time is shortened, and the accuracy is provided.

Description

technical field [0001] The invention relates to a blood detection kit and its detection method and application, in particular to a platelet antibody detection kit based on the solid-phase agglutination principle, its detection method and its application. Background technique [0002] Platelet antibodies can cause platelet transfusion ineffectiveness, post-transfusion purpura, autoimmune thrombocytopenia and neonatal alloimmune thrombocytopenia and other diseases. At present, the commonly used clinical platelet antibody detection methods mainly include solid phase erythrocyte adhesion assay (SPRCA), monoclonal antibody immobilized platelet antigen assay (MAIPA), modified antigen capture ELISA assay (MACE), and flow cytometry immunofluorescence assay ( FCM) and lymphocytotoxicity test (LCT). [0003] In order to accurately detect platelet antibodies, those skilled in the art have developed a variety of detection methods and detection reagents based on different principles, su...

AI Technical Summary

Problems solved by technology

However, for different detection methods, the corresponding detection tools (kits) are often different, and there are defects such as complex operation or need for auxiliary professional instruments or low sensitivity or short shelf life of products, etc., making it difficult to promote on a large scale

Images