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Enzymatic chemiluminescence substrate liquid of alkaline phosphatase and preparation method of liquid

An enzymatic chemiluminescence and phosphatase technology, which is applied in the field of immunoassay to achieve the effects of improving market competitiveness, long duration and high luminescence intensity

Inactive Publication Date: 2018-09-21
JIANGSU ZECEN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to overcome the defect that the existing chemiluminescent substrate solution needs to mix equal volumes of liquid A and liquid B and need to be used up within 10 minutes, and to provide an enzymatic alkaline phosphatase Chemiluminescent substrate solution and preparation method thereof

Method used

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  • Enzymatic chemiluminescence substrate liquid of alkaline phosphatase and preparation method of liquid
  • Enzymatic chemiluminescence substrate liquid of alkaline phosphatase and preparation method of liquid

Examples

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Effect test

Embodiment 1

[0018] An enzyme-catalyzed chemiluminescent substrate solution for alkaline phosphatase, containing 110mg / L of ((4-chlorophenyl)thio)(10-methylacridine-9(10H)-subunit)methylphosphoric acid Disodium, 3.8mg / L lucigenin, 1g / L sodium lauryl sulfonate, 0.5mM magnesium chloride and 10mg / L sodium sulfite.

Embodiment 2

[0020] An enzyme-catalyzed chemiluminescent substrate solution for alkaline phosphatase, containing 95mg / L of ((4-chlorophenyl)thio)(10-methylacridine-9(10H)-subunit)methylphosphoric acid Disodium, 3.0 mg / L lucigenin, 1 g / L sodium lauryl sulfonate, 0.1 mM magnesium chloride and 5 mg / L sodium sulfite.

Embodiment 3

[0022] An enzyme-catalyzed chemiluminescent substrate solution for alkaline phosphatase, containing 120mg / L of ((4-chlorophenyl)thio)(10-methylacridine-9(10H)-subunit)methylphosphoric acid Disodium, lucigenin 4.5mg / L, sodium lauryl sulfonate 1g / L, magnesium chloride 1.0mM and sodium sulfite 15mg / L.

[0023] The preparation method of the enzymatic chemiluminescence substrate solution of alkaline phosphatase of embodiment 1-3 is to first prepare the tirs solution of 0.05-0.35mol / L, add sodium dodecylsulfonate and sodium sulfite, and adjust the pH value to 8.85 ±0.1, then add ((4-chlorophenyl)thio)(10-methylacridin-9(10H)-ylidene) disodium methylphosphate and lucigenin.

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Abstract

The invention discloses enzymatic chemiluminescence substrate liquid of alkaline phosphatase. The liquid contains 95-120 mg / L of ((4-chlorphenyl)sulfo)(10-methylacridine-9(10H)-subunit)methyl disodiumphosphate, and further contains 3.0-4.5 mg / L of lucigenin, 1 g / L of sodium dodecyl sulfate, 0.1-1.0 mM of magnesium chloride and / or 5-15 mg / L of sodium sulfite. The pH value is 8.85+ / -0.1 within thetemperature range being 18-25 DEG C, the relative luminous intensity RLU is smaller than or equal to 1,500, and when the alkaline phosphatase concentration is 0.1 microgram / mL, the luminous intensityRLU value reaches 32,000,000 or above. The enzymatic chemiluminescence substrate liquid of alkaline phosphatase can reach a plateau phase rapidly within 100 s, the luminous intensity is high, the timeduration is long and can reach 30 min or above, the clinical examination requirements are met, and the main performance indexes already achieve the same level as the international standard, so that the cost of the luminous substrate is greatly lowered, and the market competitiveness of a chemiluminescent reagent and control force of a core technology are improved.

Description

technical field [0001] The invention relates to the technical field of immunoassay, in particular to an alkaline phosphatase enzyme-catalyzed chemiluminescence substrate solution and a preparation method thereof. Background technique [0002] The enzyme-catalyzed chemiluminescent substrate solution belongs to the technical field of immunodiagnosis. In the mid-1970s, Arakawe first reported the use of luminescent signals for enzyme immunoassay, using luminescent chemical reactions to analyze ultra-trace substances, especially for the detection of ultra-trace active substances in clinical immunoassays. At present, this technology has become a routine detection method in clinical medicine. Chemiluminescence Immunoassay (CLIA) is a new type of labeled immunoassay technology that combines chemiluminescence or bioluminescence system with immune reaction to detect trace antigen or antibody. Its detection principle is similar to that of radioimmunoassay (RIA) and enzyme immunoassay...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/531G01N21/76
CPCG01N21/76G01N33/531
Inventor 郭翠刘振世刘振华程云燕倪磊
Owner JIANGSU ZECEN BIOTECH CO LTD
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