A Method for the Detection of Sudan Red I by Vortex-Assisted Dispersion Liquid-Liquid Microextraction in a Hydrophobic Deep Eutectic Solvent-High Performance Liquid Chromatography
A technology of high-performance liquid chromatography and deep eutectic solvent, which is applied in the field of environmental analysis, can solve problems such as unfavorable operators and the environment, and achieve the effect of broadening the scope of application and reducing hazards
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Embodiment 1
[0021] Detection of Sudan Red I in Environmental Water Samples
[0022] 1. Instrument: Waters high performance liquid chromatograph equipped with 1525 pump, model 1500 column thermostat and 2998 photodiode array detector; Waters SunFire C18 liquid chromatography column (150mm×4.6mm, particle size 5μm), The mobile phase is methanol / water (95 / 5, v / v), the flow rate is 1.0 mL / min, the column temperature is 35 °C, the injection volume is 20 μL, and the detection wavelength is 478 nm; hydrogen bonds in the hydrophobic eutectic solvent The molar ratio of the acceptor compound methyl trioctyl ammonium chloride to the hydrogen bond donor compound oleic acid is 1:2.
[0023] 2. Sample processing: The sample to be tested is an environmental water sample. The environmental water sample includes tap water, river water, well water or lake water. The environmental water sample is filtered with a 0.45μm water phase microporous membrane before analysis and testing.
[0024] 3. Standard curve drawin...
Embodiment 2
[0031] Detection of Sudan Red I in Food Samples
[0032] 1. Apparatus and reagents: Waters high performance liquid chromatograph, equipped with 1525 pump, model 1500 column thermostat and 2998 photodiode array detector; Waters SunFire C18 liquid chromatography column (150mm×4.6mm, particle size 5μm ), the mobile phase is methanol / water (95 / 5, v / v), the flow rate is 1.0 mL / min, the column temperature is 35 °C, the injection volume is 20 μL, and the detection wavelength is 478 nm; in a hydrophobic eutectic solvent The molar ratio of the hydrogen bond acceptor compound methyl trioctyl ammonium chloride to the hydrogen bond donor compound oleic acid is 1:2.
[0033] 2. Sample processing: The sample to be tested is a food sample, and the food sample is chili powder or chili noodles. Before analysis and testing, place 2g of the food sample in a plastic centrifuge tube, add 20mL n-hexane, ultrasound for 5min, filter, and use 10mL n-hexane The residue was washed with alkane several times,...
Embodiment 3
[0038] Detection of Sudan Red I in Biological Samples
[0039] 1. Instrument: Waters high performance liquid chromatograph equipped with 1525 pump, model 1500 column thermostat and 2998 photodiode array detector; Waters SunFire C18 liquid chromatography column (150mm×4.6mm, particle size 5μm), The mobile phase is methanol / water (95 / 5, v / v), the flow rate is 1.0 mL / min, the column temperature is 35°C, the injection volume is 20 μL, and the detection wavelength is 478 nm; hydrogen bonds in the hydrophobic eutectic solvent The molar ratio of the acceptor compound methyl trioctyl ammonium chloride to the hydrogen bond donor compound oleic acid is 1:2.
[0040] 2. Sample processing: The sample to be tested is a biological sample. The biological sample is duck blood or pig blood. Put 5g of the biological sample in a plastic centrifuge tube before analysis and detection, add 15mL n-hexane, vortex for 5min, and then rotate at 8000rpm Centrifuge at a centrifugal speed for 5 minutes, aspira...
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