Pteris vittata spore germination and intensive seedling culture method

A technology for spore germination and grass spores, which is applied in the field of inducing fern spore germination and seedling cultivation, can solve problems such as difficult practical application, complicated methods, and small centipede grass spores, and achieve the effect of shortening the seedling cultivation cycle and easy operation

Active Publication Date: 2018-09-28
HUNAN INST OF AGRI ENVIRONMENT & ECOLOGY
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Problems solved by technology

[0002]The spores of Centipede Grass are small, it is difficult to control the number of sowing, and the high-density sowing of spore powder is likely to cause seedling failure due to mold infection
[0003] Previously, there were some reports on the breeding methods of centipede grass, but the results were not good
Some researchers have made related attempts by using tissue culture methods, such as Chinese patent CN200510086732.8, but these methods are relatively com...

Method used

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  • Pteris vittata spore germination and intensive seedling culture method
  • Pteris vittata spore germination and intensive seedling culture method

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Embodiment 1

[0039] The first step, collecting the spores of Centipede spores: Harvest the spores of Centipedes spores with mature spores, dry them in the shade, knead and crush them, pass through a 100-mesh fine sieve, remove the mixed stem and leaf debris in the spore powder, and collect them to obtain Pure spore powder is refrigerated at -20°C refrigerator;

[0040] The second step, the preparation of spore germination culture solution: the formula is 0.5‰ agarose + 1mg / L gibberellin GA3 + 0.1mg / L naphthalene acetic acid NAA solution, the pH value is adjusted to 7.5-8.0, and then after 120 ℃ high temperature Sterilize for 20 minutes.

[0041] The third step is to induce the germination of centipede grass spores: pour 1g of centipede grass spore powder into 100mL of spore germination culture medium, oscillate with a vortex shaker to make the spores evenly suspended in the culture medium, put it into a shaking incubator at 25°C for 30-50 rpm Shake per minute and incubate in the dark for ...

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Abstract

The invention provides a pteris vittata spore germination and intensive seedling culture method. The method comprises the following steps: (1) placing pteris vittata spore powder in a spore germination culture solution, and culturing under a dark condition until the spore is germinated and rooted; (2) uniformly planting the germinated and rooted spore treated by the step (1) in a seedling culturematrix, culturing until the pteris vittata leaves grow; (3) after the step (2), fertilizing at an appropriate time, performing the necessary field management, culturing for 4 to 5 months, and then transplanting into the field. The seedling culture method is simple in operation, shortens the seedling culture period, overcomes the weaknesses in the prior art, facilitates the mass and intensive seedling culture of the ultra-accumulation plant pteris vittata, and can provide a great amount of high-quality low-price ultra-accumulation plant pteris vittata seedlings to restore the arsenic pollutionof the soil in farmland.

Description

technical field [0001] The invention belongs to the technique of inducing fern spore germination and raising seedlings, and relates to a method for the germination and intensive seedling raising of centipede spores. Background technique [0002] Centipede grass spores are small, it is difficult to control the number of sowing, and high-density sowing of spore powder is easy to cause seedling failure due to mold infection. [0003] Previously, there were some reports on the methods of breeding centipede grass, but the results were not good. Some researchers have made related attempts by using tissue culture methods, such as Chinese patent CN200510086732.8, but these methods are relatively complicated and difficult to apply in practice. [0004] The Institute of Geographical Sciences and Natural Resources Research of the Chinese Academy of Sciences announced a method for industrialized seedling cultivation of centipede grass in its Chinese patent CN201510937385.9, but this me...

Claims

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Application Information

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IPC IPC(8): A01G31/00A01C1/00
CPCA01C1/00A01G31/00
Inventor 陈锦贺爱国纪雄辉吴家梅谢运河官迪陈山
Owner HUNAN INST OF AGRI ENVIRONMENT & ECOLOGY
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