Pteris vittata spore germination and intensive seedling culture method
A technology for spore germination and grass spores, which is applied in the field of inducing fern spore germination and seedling cultivation, can solve problems such as difficult practical application, complicated methods, and small centipede grass spores, and achieve the effect of shortening the seedling cultivation cycle and easy operation
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[0039] The first step, collecting the spores of Centipede spores: Harvest the spores of Centipedes spores with mature spores, dry them in the shade, knead and crush them, pass through a 100-mesh fine sieve, remove the mixed stem and leaf debris in the spore powder, and collect them to obtain Pure spore powder is refrigerated at -20°C refrigerator;
[0040] The second step, the preparation of spore germination culture solution: the formula is 0.5‰ agarose + 1mg / L gibberellin GA3 + 0.1mg / L naphthalene acetic acid NAA solution, the pH value is adjusted to 7.5-8.0, and then after 120 ℃ high temperature Sterilize for 20 minutes.
[0041] The third step is to induce the germination of centipede grass spores: pour 1g of centipede grass spore powder into 100mL of spore germination culture medium, oscillate with a vortex shaker to make the spores evenly suspended in the culture medium, put it into a shaking incubator at 25°C for 30-50 rpm Shake per minute and incubate in the dark for ...
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