Preparation process and preparation device of polypeptide compound
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A preparation process and technology for peptide compounds, which are applied in the field of preparation technology and preparation device of polypeptide compounds, can solve the problems of low extraction efficiency and low purity of extraction methods, and achieve the effects of good quality and high efficiency.
Inactive Publication Date: 2018-09-28
郑娟娟
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[0003] At present, there are many ways to extract velvet antler polypeptides, some of which have low extraction efficiency, and some extraction methods are not high in purity.
Method used
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Embodiment 1
[0024] A preparation process of a polypeptide compound, characterized in that it comprises the following steps:
[0025] 1) 100kg velvet slices are frozen to form a frozen object;
[0026] 2) crushing the frozen object with a pulverizer to obtain a crushed object;
[0027] 3) Add the ground material to 350L of ethanol with a concentration of 85% to obtain a mixture;
[0028] 4) Add 12mol / L hydrochloric acid to the mixture, adjust the pH to 2.5-2.8, and keep stirring for more than 6 hours;
[0029] 5) filter off the residue to obtain a primary extract;
[0030] 6) The extract is cooled to below 3°C, and the pH is adjusted to 6.8-7.8 with concentrated ammonia water to obtain an alkaline extract;
[0031] 7) Add 8 kg of diatom ooze to the alkaline extract, press filter, add 2000 ml of sulfuric acid and 6000 ml of water for acidification, filter out the residue after acidification, and obtain a secondary extract again.
[0032] 8) adding 4 kg of zinc fluoride to the secondary ...
Embodiment 2
[0037] A preparation process of a polypeptide compound, characterized in that it comprises the following steps:
[0038] 1) 150kg velvet slices are frozen to form a frozen object;
[0039] 2) crushing the frozen object with a pulverizer to obtain a crushed object;
[0040] 3) Add the ground material to 350L of ethanol with a concentration of 95% to obtain a mixture;
[0041] 4) Add 12mol / L hydrochloric acid to the mixture, adjust the pH to 2.5-2.8, and keep stirring for more than 8 hours;
[0042] 5) filter off the residue to obtain a primary extract;
[0043] 6) The extract is cooled to below 3°C, and the pH is adjusted to 6.8-7.8 with concentrated ammonia water to obtain an alkaline extract;
[0044] 7) Add 10kg of diatom ooze to the alkaline extract, press filter, add 3000ml of sulfuric acid and 8000ml of water for acidification, filter off the residue after acidification, and obtain the secondary extract again.
[0045] 8) adding 6 kg of zinc fluoride to the secondary ...
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Abstract
The invention discloses a preparation process of a polypeptide compound. The process comprises the following steps of freezing and crushing raw material slices; adding ethanol to obtain a mixture; adding the mixture into hydrochloric acid; performing stirring; performing filtering; cooling extraction liquid to the temperature of 3 DEG C or below; regulating the pH by stronger ammonia water to obtain basic extraction liquid; adding diatom ooze into the basic extraction liquid; performing press filtering; adding sulfuric acid for acidification; after the acidification, filtering away residues; adding zinc fluoride; adding hydrochloric acid for acidification; filtering away residues again to obtain extraction liquid filtered for three times and freezing to 5 DEG C or below; removing precipitates; regulating the pH to 5 to 6.0 by ammonia water; adding zinc acetate to obtain white precipitates; then, adding a mixture of hydrochloric acid, zinc sulfate and acetone; cooling crystalline liquid; performing alkalization by ammonia water; performing filtering to remove precipitates; performing stirring and crystallization; next, performing hydrolysis by pepsin and pancreatin; after enzyme deactivation, obtaining pilose antler protein hydrolysates; next, performing separation and purification to obtain a polypeptide mixture. The polypeptide mixture preparation efficiency of the process provided by the invention is high.
Description
technical field [0001] The invention relates to a preparation process and a preparation device of a polypeptide compound. Background technique [0002] Antler polypeptide is a protein with small molecules, strong activity, easy absorption, and small side effects. According to research, velvet antler polypeptide has anti-inflammatory, anti-oxidation, enhanced immunity, and can promote cell differentiation, wound healing, inhibit tumor cells, and promote nervous system peripheral nerves. It is one of the most effective ingredients in velvet antler for its pharmacological effects such as regeneration of systemic tissues. The high-purity single peptide obtained by separation and purification has been used as an emerging frontier drug in contemporary times. [0003] At present, there are many ways to extract velvet antler polypeptides, some of which have low extraction efficiency, and some of which have low purity. Contents of the invention [0004] In order to overcome above...
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