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Test paper for rapidly detecting uric acid in saliva

A technology for detecting test strips and saliva, which is applied in the direction of material analysis by observing the influence of chemical indicators, and analysis by making materials undergo chemical reactions. It can solve the problems of invasive price, high cost, and complicated uric acid detection process. Low cost, good stability, conducive to large-scale production

Inactive Publication Date: 2018-09-28
SUN YAT SEN UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0004] Aiming at the shortcomings of complicated, invasive and expensive uric acid detection process at the present stage, the present invention provides a detection test paper for rapid detection of saliva uric acid. When the test paper block is in contact, the enzyme chromogen solution on the test paper block will react with uric acid, and the quinone imine compound will be generated according to the color reaction principle of the coupling terminal colorimetry method. The color concentration of the quinone imine compound is directly proportional to the uric acid content. , and because of the correlation between saliva uric acid concentration and serum uric acid concentration, the serum uric acid concentration can be further judged by the color change of the test strip, providing a safer, economical and convenient means for monitoring health

Method used

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  • Test paper for rapidly detecting uric acid in saliva

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Embodiment 1

[0042] This embodiment provides a detection test paper for rapid detection of sialouric acid, comprising a plastic cardboard 1 and a test paper block 2 pasted on one end of the plastic card paper 1, the test paper block 2 is impregnated with an enzyme developer solution, the The test paper block 2 can be selected from any medium-speed quantitative filter paper or cellulose paper. In the present embodiment, the test paper block 2 is selected from a medium-speed quantitative filter paper, and the size of the test paper block 2 is 5 mmⅹ5 mm. The test paper block 2 and plastic Cardstock 1 is pasted with double-sided tape. Wherein, the enzyme chromogen solution uses a borate standard buffer solution as a dissolving solution, and the pH value of the borate standard buffer solution is 8.5. The enzyme chromogen solution specifically includes uricase, horseradish catalase, 4-aminotipyrine, 3-hydroxy-2,4,6-tribromobenzoic acid and 2,3-dichloro-5-trifluoro picoline, and the concentratio...

Embodiment 2

[0050] This embodiment is similar to the embodiment, further, the enzyme chromogen solution specifically includes uricase, horseradish catalase, 4-aminotipyrine, 3-hydroxy-2,4,6-tribromobenzene Formic acid, 2,3-dichloro-5-trifluoromethylpyridine, bilirubin oxidase and gelatin solution, the concentration of the gelatin solution is 1.6 mg / mL-2.0 mg / mL. The content of the above-mentioned components in the enzyme developer solution is as follows: the concentration of uricase is 0.15 KU / L-0.3 KU / L; the concentration of horseradish catalase is 2.0 KU / L-3.5KU / L; The concentration of tipyrine is 100 mg / L; the concentration of 3-hydroxy-2,4,6-tribromobenzoic acid is 4 g / L-6 g / L; 2,3-dichloro-5-trifluoromethyl The concentration of pyridine is 1.5 g / L-2 g / L; the concentration of bilirubin oxidase is 0.05 g / L-0.2 g / L; the gelatin solution is 0.5 mL. In this embodiment, the preferred concentration of each component in the enzyme developer solution is as follows: the concentration of urica...

Embodiment 3

[0052] This embodiment provides a method for preparing a detection test paper for rapid detection of sialuric acid, which specifically includes the following steps:

[0053] S1. The preparation of gelatin solution, first add an appropriate amount of gelatin in ultra-clean water to foam for 2 hours, and then dissolve it at 60 °C to form a gelatin solution with a concentration of 1.6 mg / mL-2.0 mg / mL. The concentration of the gelatin solution is optimal 1.8 mg / mL;

[0054] S2, the preparation of enzyme chromogen solution, with the borate standard buffer solution with a pH value of 8.5 as the dissolving solution, add appropriate amount of uricase, horseradish catalase, 4-aminotipyrine, 3-hydroxy - 2,4,6-tribromobenzoic acid, 2,3-dichloro-5-trifluoromethylpyridine, bilirubin oxidase and gelatin solution, mixed evenly to form an enzyme developer solution;

[0055] S3. Fully immerse the test paper block 2 in the enzyme chromogen solution for 30-40 min, then air-dry it under low temp...

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Abstract

The invention relates to a test paper for rapidly detecting uric acid in saliva. The test paper comprises a plastic card paper and a test paper block pasted to one end of the plastic card paper; and the test paper block is impregnated with an enzyme developer solution. The test paper is produced through pasting the test block impregnated with the enzyme developer solution to one end of the plasticcard paper. A production technology of the test paper has the advantages of simplicity, low cost, and facilitation of large-scale production. Additionally, the detection process realizes semi-quantitative detection of the concentration of uric acid based on the color development reaction principle of coupled endpoint colorimetry, the test paper ahs a good stability at 35 DEG C, is convenient fora patient to autonomously carry in order to monitor the uric acid, and is beneficial for the patient to timely find the disease condition and select optimum treatment timing. The test paper is also suitable for monitoring uric acid in urine.

Description

technical field [0001] The invention relates to a test paper for detecting uric acid, in particular to a test paper for rapidly detecting uric acid in saliva. The detection test paper is made based on the color reaction principle of the coupled end-point colorimetric method, and can quickly and semi-quantitatively detect the concentration of uric acid in saliva. Background technique [0002] In recent years, with the rapid development of my country's economy and the improvement of living standards, the dietary structure has also undergone significant changes. The intake of protein and foods rich in purine components has increased, and the prevalence of hyperuricemia has increased year by year. The prevalence of kidney disease has also increased. Many studies at home and abroad have confirmed that hyperuricemia is closely related to the occurrence of gout, cardiovascular disease, metabolic syndrome, hypertension and kidney disease, and is an independent risk factor for the oc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 周建华林茂仁王瑛姝婷黄文威罗崇岱
Owner SUN YAT SEN UNIV
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