Nervilia fordii tissue culture and rapid propagation method

A technology of tissue culture and blue skyflower, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve problems such as low reproduction coefficient and exhaustion of wild blue skyflower resources, reduce cultivation steps, solve large-scale seedling cultivation, and realize The effect of mass production

Inactive Publication Date: 2018-10-02
佛山市元诺科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The blue skyflower mainly reproduces by bulbs, but the reproduction coefficient is extremely low. In addition, people have been digging extinctly for many years, which has led to the depletion of wild blue skyflower resources.
However, the demand in domestic and foreign markets is increasing year by year, and Qingtiankui is far from meeting the demand of the market.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] An example of the rapid propagation method of blue skyflower tissue culture of the present invention, comprises the steps:

[0020] (1) Extraction and disinfection of explants: As the explants, the bulbs of the blue skyflower are sterilized. First, 3-5 drops of detergent are dripped into a beaker with 50ml of tap water, and the explants are put into the beaker and stirred gently. 5min, then use cotton to clean the dirt on the surface of the explant, rinse with tap water for 20-30min; move the ultra-clean bench, soak in 75v / v% ethanol for 30s, rinse with sterile water, and then add 1-2 Sterilize 50ml of Tween-20 with 0.1v / v% mercuric chloride for 1-2min, rinse with sterile water for 3-5 times to obtain sterile explants, wherein the sterile water is distilled water sterilized by high temperature and high pressure ;

[0021] (2) Acquisition of sterile rhizomes: Place the sterilized explants in the induction medium for adventitious bud induction, at a temperature of 25-27°...

Embodiment 2

[0028] Another example of the method for rapid propagation of blue skyflower tissue culture of the present invention, comprises the steps:

[0029] Other conditions are the same as embodiment 1, only the following content is different:

[0030] In step (3), the integrated medium for rhizome propagation and bulb induction is 1 / 2MS as the basic medium, and 5g L-1 agar, 20g L-1 sucrose, 1.5mg L-1 of 6 - benzyl adenine, 0.3mg·L-1 kinetin (KT), 0.3mg·L-1 naphthaleneacetic acid, the pH value of the medium is 6.8; the bulb induction coefficient is 21.

[0031] In step (4), the transplanting survival rate was 93%.

Embodiment 3

[0033] Another example of the method for rapid propagation of blue skyflower tissue culture of the present invention comprises the following steps:

[0034] Other conditions are the same as embodiment 1, only the following content is different:

[0035] In step (3), the integrated medium for rhizome propagation and bulb induction is 1 / 2MS as the basic medium, and 5g L-1 agar, 20g L-1 sucrose, 1.5mg L-1 of 6 - Benzyl adenine, 0.5 mg·L-1 kinetin (KT), 0.5 mg·L-1 naphthalene acetic acid, the pH value of the medium is 6.8; the bulb induction coefficient is 24.4.

[0036] In step (4), the transplanting survival rate was 91.5%.

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PUM

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Abstract

The invention discloses a nervilia fordii tissue culture and a rapid propagation method which is characterized by comprising the following steps: (1) selecting and disinfecting explants; (2) obtainingsterile rhizomes; (3) performing propagation of rhizomes and corm-induced integrated culture; (4) performing corm seedling hardening and transplanting. Compared with the prior art, the method in thetechnical scheme of the invention has the beneficial effects that nervilia fordii corms are subjected to tissue culture and rapid propagation by virtue of a biotechnology, lots of nervilia fordii seedlings for field production can be cultivated in a short time, the propagation coefficient and quality of the nervilia fordii seedlings are obviously improved, large-scale production is realized, and production needs are met.

Description

technical field [0001] The invention relates to a rapid propagation method of blue skyflower tissue culture. Background technique [0002] Blue Sky Kwai is the leaf or bulbous leaf of orchid plant Nerviliafordii (Hance) Schitr, also known as unifoliate, uniped lotus, pearl leaf, falling jack, iron hat, samosa, and green lotus. Flat in nature, bitter in the mouth, sweet, cool. Astragalus is a rare and endangered plant in my country. It has the functions of clearing the lung and relieving cough, strengthening the spleen and eliminating stagnation, clearing away heat and detoxification, eliminating stagnation and dispelling pain. Scrofula, sores, swollen toxins, bruises, etc. The blue skyflower is mainly propagated by bulbs, but the reproduction coefficient is extremely low. In addition, the extinction-style excavation by people over the years has led to the depletion of wild blue skyflower resources. However, the demand in domestic and foreign markets is increasing year by y...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 袁萍
Owner 佛山市元诺科技有限公司
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