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A kind of compound modification method of laccase and its application

A composite modification and laccase technology, applied in the field of laccase modification, can solve the problems of poor laccase modification activity modification effect, etc., and achieve the effect of improving the performance of pulp and paper making

Active Publication Date: 2022-03-25
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved in the present invention is to overcome the defects and deficiencies in the prior art that the modification effect of laccase modification activity is not good, and provide a method for compound modification of laccase, by simultaneously modifying the laccase amino acid residue side chain Amino and carboxyl, greatly improving the modification effect of chemical modification

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Examples 1 to 11

[0045] A laccase composite modification method, comprising the steps:

[0046] S1. Carboxyl group modification: successively add a laccase inhibitor, a carboxyl group activator, and a modifier to the laccase aqueous solution to react, and after the reaction is complete, the reaction system is dialyzed to obtain a carboxyl group-modified laccase;

[0047] The modifier is formamidine acetate solution prepared from potassium dihydrogen phosphate / dipotassium hydrogen phosphate (pH=8.01) buffer solution, and the aqueous laccase solution is 10 mL (50 mg / mL) of laccase solution prepared with distilled water. , the carboxyl activator is 10 mg 2-ethyl-5-phenylisoxazole-3'-sulfonate (1 mg / mL),

[0048] After reacting at 25°C for 1 h, 12 mL of acetic acid / sodium acetate (pH=4.79) buffer solution was added to terminate the reaction, the solution was put into a dialysis bag, and the solution was added in potassium dihydrogen phosphate / dipotassium hydrogen phospha...

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PUM

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Abstract

The invention discloses a method for compound modification of laccase and its application. The method for compound modification of laccase comprises the following steps: carboxyl modification: successively add laccase inhibitor, carboxyl activator and modifier to the laccase aqueous solution for reaction, and then dialyze the reaction system to obtain carboxyl modified laccase after the reaction is complete; Modification: add an amino activator and a modifier to the carboxyl-modified laccase solution in S1 successively for reaction, and dialyze after the reaction is complete to obtain a composite modified laccase. The compound modification of laccase of the present invention carries out compound modification to the carboxyl group and amino group on the amino acid residue side chain or terminal amino acid of laccase, protects the active group carboxyl group and amino group on the laccase chain at the same time, and protects the active center of laccase Under the premise that the site is not affected, the activity and stability of the laccase can be changed, and the obtained composite modified laccase can significantly improve the pulping and papermaking performance of the modified fiber, and provide a new way for fiber reuse.

Description

technical field [0001] The invention belongs to the technical field of laccase modification, and more particularly relates to a laccase composite modification method and application thereof. Background technique [0002] Royal bamboo grass is a large-yielding plant, which has the characteristics of strong adaptability, strong tillering, fast growth, high yield, rich nutrition, high fiber content, and can be harvested for many years. It is a valuable non-wood fiber pulping and papermaking. raw material, but the strength of the paper sheet is low, in order to improve its utilization level and use value, it is necessary to modify it. In terms of fiber modification, biomodification of laccase mediator system is a hot and difficult research topic at present. Biomodification of laccase mediator system has many advantages, such as mild reaction conditions, less fiber damage, and the use of environmentally friendly drugs, etc. , but because laccase has amino acid residues or termin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/02D06M16/00D06M13/342
CPCC12N9/0061C12Y110/03002D06M13/342D06M16/003D06M2101/04
Inventor 陈杨梅蒋雨吴启堂陈家进仇卓涛陈烁娜
Owner SOUTH CHINA AGRI UNIV