Method for determining fermentation capability of functional microbial carrier

A technology of functional microorganisms and fermentation capacity, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, and microbial measurement/inspection. It can solve the problems of limited types of organic fertilizers and the inability to accurately detect changes in the number of functional microorganisms. To achieve the effect of improving the screening efficiency

Active Publication Date: 2018-10-12
GUIZHOU TOBACCO SCI RES INST
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Problems solved by technology

[0004] The technical problem to be solved in the present invention is to provide a method for measuring the fermentation capacity of functional microbial carriers, by comparing the difference between the characteristic carbon and nitrogen sources of functional strains and the characteristic carbon and nitrogen sources of bio-organic fertilizers after fermentation, it can be Quickly determine the fermentation capacity of functional microbial carriers to solve the problems of the traditional inoculation method, such as the limited types of organic fertilizers to be tested, and the inability to accurately detect changes in the number of functional microorganisms

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  • Method for determining fermentation capability of functional microbial carrier
  • Method for determining fermentation capability of functional microbial carrier
  • Method for determining fermentation capability of functional microbial carrier

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Embodiment Construction

[0024] Below in conjunction with accompanying drawing and specific embodiment the invention is further introduced:

[0025] 1. Embodiment

[0026] A method for measuring the fermentative capacity of a functional microbial carrier according to the present invention comprises the following steps:

[0027] (1) Preparation of functional bio-organic fertilizer

[0028] Inoculate the purified microbial strains (such as Bacillus amyloliquefaciens, Bacillus subtilis, Bacillus brevis, Pseudomonas, Bacillus licheniformis, Bacillus megaterium, etc.) in beef extract peptone liquid medium, shake at 30°C and 170rpm for 48h . The fermentation broth was centrifuged at 6000×g for 10 min to collect the bacteria. The bacterium is inoculated on different organic fertilizer carriers (such as cow dung organic fertilizer, oil dry organic fertilizer, etc.) according to the inoculum amount of 5%. Adjust the water content of the organic fertilizer to 40%, and ferment for 5-6 days to obtain a functi...

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Abstract

The invention discloses a method for determining the fermentation capability of a functional microbial carrier. The method comprises the following steps that 1, a functional strain is inoculated to anorganic fertilizer carrier, and a functional bio-organic fertilizer for use is obtained through fermentation; 2, the distinctive carbon and nitrogen sources of the functional strain and the functional bio-organic fertilizer are determined, the area of the carbon and nitrogen sources which can be metabolized by the functional strain is used for reference and is marked with a red color, the area ofthe carbon and nitrogen sources which can be metabolized by the functional bio-organic fertilizer is used for detection and is marked with a green color, and the area of the carbon and nitrogen sources, having the same capacity, of the functional strain and the functional bio-organic fertilizer are marked with a yellow color; 3, the fermentation capability of an organic fertilizer carrier is calculated. According to the method, by comparing the difference between the distinctive carbon and nitrogen sources of functional microbes and the distinctive carbon and nitrogen sources of the fermentedbio-organic fertilizer, the fermentation capability of the microbial carrier can be quickly determined, thus the best carrier for fermentation of the functional microbes can be quickly determined, and the screening efficiency of the functional microbial fermentation carrier is greatly improved.

Description

technical field [0001] The invention relates to functional microorganisms, in particular to a method for measuring the fermentation capacity of functional microorganism carriers. Background technique [0002] At present, the main problems facing my country's soil are: slow transformation of soil nutrients, accumulation of soil pollutants and frequent occurrence of soil-borne diseases. The application of organic fertilizers, especially bio-organic fertilizers with special functions, has become a new idea for repairing soil in tobacco fields and preventing various soil-borne diseases and continuous cropping obstacles. However, functional strains are not easy to colonize when applied alone in the soil. Inoculating them in suitable organic fertilizers for secondary solid fermentation to make functional bio-organic fertilizers can improve the colonization ability of functional strains in soil and rhizosphere , so as to achieve the purpose of regulating soil microecology, improvi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/02C12R1/07
CPCC12Q1/02G01N2333/32
Inventor 刘艳霞李想石俊雄张恒
Owner GUIZHOU TOBACCO SCI RES INST
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