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Preparation method of Yupingfeng polysaccharide lipidosome

A kind of jade screen and liposome technology, which is applied in the directions of liposome delivery, medical preparations of inactive ingredients, pharmaceutical formulations, etc., can solve the problems of unsatisfactory effect, poor controllability, complicated process, etc., and achieve excellent stability and cell penetration

Inactive Publication Date: 2018-10-16
FOSHAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, traditional Yupingfeng polysaccharide preparations have disadvantages such as poor stability, low bioavailability, easy degradation, and weak cell penetration. The effect is not good
In the prior art, the polysaccharides of Yupingfeng are mostly modified to improve its biological activity so as to better exert its medicinal effect. However, the effect of these modifications is not ideal, and the process is complicated and the controllability is poor.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A preparation method of Yupingfeng polysaccharide liposome, which comprises the following processing steps:

[0024] 1) Purification of Yupingfeng polysaccharide: take Yupingfeng crude polysaccharide and dissolve it in distilled water, then slowly pour it into a resin column, the resin volume in the resin column is 2 / 3 of the volume of the resin column, repeat several times, and then add 50% ethanol Slowly pour into the resin column, and then use a vacuum filtration pump to make the eluate flow into the filter bottle, collect the eluate in the filter bottle, concentrate and vacuum dry for 12 hours to obtain purified Yupingfeng polysaccharide powder with a purity of ≥80%. ;

[0025] 2) Liposome preparation: Soybean lecithin and cholesterol were dissolved in chloroform solution at a mass ratio of 7:1, and then subjected to reduced-pressure rotary evaporation at a rotation speed of 75r / min and a temperature of 37°C. , after forming a thin film, add ether film to dissolve ...

Embodiment 2

[0030] A preparation method of Yupingfeng polysaccharide liposome, which comprises the following processing steps:

[0031] 1) Purification of Yupingfeng polysaccharide: take Yupingfeng crude polysaccharide and dissolve it in distilled water, then slowly pour it into a resin column, the resin volume in the resin column is 2 / 3 of the volume of the resin column, repeat several times, and then add 50% ethanol Slowly pour into the resin column, and then use a vacuum filtration pump to make the eluate flow into the filter bottle, collect the eluate in the filter bottle, concentrate and vacuum dry for 12 hours to obtain purified Yupingfeng polysaccharide powder with a purity of ≥80%. ;

[0032] 2) Liposome preparation: Soybean lecithin and cholesterol were dissolved in chloroform solution at a mass ratio of 9:1, and then subjected to reduced-pressure rotary evaporation at a rotational speed of 60r / min and a temperature of 37°C. , after forming a thin film, add ether film to dissolv...

Embodiment 3

[0037] A preparation method of Yupingfeng polysaccharide liposome, which comprises the following processing steps:

[0038] 1) Purification of Yupingfeng polysaccharide: take Yupingfeng crude polysaccharide and dissolve it in distilled water, then slowly pour it into a resin column, the resin volume in the resin column is 2 / 3 of the volume of the resin column, repeat several times, and then add 50% ethanol Slowly pour into the resin column, and then use a vacuum filtration pump to make the eluate flow into the filter bottle, collect the eluate in the filter bottle, concentrate and vacuum dry for 12 hours to obtain purified Yupingfeng polysaccharide powder with a purity of ≥80%. ;

[0039] 2) Liposome preparation: Soybean lecithin and cholesterol were dissolved in chloroform solution at a mass ratio of 8:1, and then subjected to vacuum rotary evaporation at a rotation speed of 70r / min and a temperature of 37°C. , after forming a thin film, add ether film to dissolve to obtain ...

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Abstract

The invention discloses a preparation method of Yupingfeng polysaccharide lipidosome. The preparation method comprises the following steps: (1) purifying Yupingfeng polysaccharide; (2) preparing lipidosome; (3) preparing a Yupingfeng polysaccharide buffering solution; and (4) preparing lipidosome. The Yupingfeng polysaccharide lipidosome is prepared by adopting a reversing evaporation method, by performing the preliminary purification of the Yupingfeng polysaccharide and adjusting the proportion of all raw materials as well as process parameters, an enveloping rate of the Yupingfeng polysaccharide lipidosome suspension reaches up to 86 percent or more; and moreover, the prepared Yupingfeng polysaccharide lipidosome has excellent stability and cellar permeability, thereby having important clinical significance for improving the biological utilization rate and reducing the application dose of the Yupingfeng polysaccharide, and further effectively promoting the research and development ofnovel preparations of the Yupingfeng polysaccharide.

Description

technical field [0001] The invention relates to the field of chemical industry and medicine, in particular to a preparation method of liposome. Background technique [0002] Yupingfeng polysaccharide is composed of active polysaccharides such as Astragalus polysaccharide, Fangfeng polysaccharide and Atractylodes polysaccharide. According to relevant research, Yupingfeng polysaccharide can promote the growth and development of human and animal thymus, spleen and other immune organs, enhance the activity of immune cells, improve the vaccination effect of various vaccines and delay the duration of antibodies. Adjustment has unique advantages. However, traditional Yupingfeng polysaccharide preparations have disadvantages such as poor stability, low bioavailability, easy degradation, and weak cell penetration. The curative effect is not good. In the prior art, the polysaccharides of Yupingfeng are mostly modified to improve its biological activity so as to better exert its med...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/127A61K47/24A61K47/28A61K31/715A61P37/04
CPCA61K9/127A61K31/715A61K47/24A61K47/28A61P37/04
Inventor 杨鸿张勇军邓桦王少杰巴娟巫辅达
Owner FOSHAN UNIVERSITY
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