Method of enriching and purifying total flavonoids of all grass of cardamine tangutorum

A technology of total flavonoids and shigecai, which is applied to the digestive system, pharmaceutical formulations, medical preparations containing active ingredients, etc., to achieve the effect of stable and feasible process

Inactive Publication Date: 2018-10-16
SOUTHWEST UNIVERSITY FOR NATIONALITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the prior art, mainly focus on the separation research of the chemical constituents of the broken rice chestnuts in central China, the white flowered rice chestnuts and the purple flowered rice chestnuts produced in

Method used

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  • Method of enriching and purifying total flavonoids of all grass of cardamine tangutorum
  • Method of enriching and purifying total flavonoids of all grass of cardamine tangutorum
  • Method of enriching and purifying total flavonoids of all grass of cardamine tangutorum

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Embodiment Construction

[0021] 1. Instruments and materials

[0022] 1 instrument

[0023] METTLER AE240 electronic balance (Mettler-Toledo (Shanghai) Co., Ltd.); IKARV10 rotary evaporator (German IKA company); DHG-9246 electric constant temperature blast drying oven (Shanghai Jinghong Experimental Equipment Co., Ltd.); Analyze TU-1950 ultraviolet spectrophotometer (Beijing General Analysis Company); PHS-3 laboratory PH meter (Shanghai Jinmai Instrument Company).

[0024] 2 Materials and reagents

[0025] The sample of Shige Cai was self-collected on July 23, 2016 in Meiwo Township, Xiaojin County, Aba Tibetan and Qiang Autonomous Prefecture, Sichuan Province, at an altitude of 3600m. Dry, crush, and pass through a No. 3 sieve for later use.

[0026] Rutin standard product (batch number: 100080-200707, China Institute for the Control of Pharmaceutical and Biological Products); macroporous adsorption resin AB-8, D101, HPD450 (batch number: 20160408, Tianjin Guangfu Fine Chemical Research Institute)...

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Abstract

The invention relates to a method of enriching and purifying total flavonoids of all grass of cardamine tangutorum. The method comprises the following steps: (1) preparing extract of the all grass ofcardamine tangutorum; (2) loading the extract of the all grass of cardamine tangutorum on a macroporous resin adsorption column, wherein the loading quantity is 8-48ml of the extract for each gram ofdry macroporous resin, and the flow is 3-4BV/h; after the loading, washing with water and then eluting with ethanol, wherein the volume fraction of ethanol is 10-90%, and the flow is 2-8BV/h; collecting the eluent and detecting the content of total flavonoids. The technology is stable and feasible, provides test data and scientific basis for the enrichment and purification of the total flavonoidsof the all grass of cardamine tangutorum, and lays a foundation for further studying the active chemical ingredients in the all grass of cardamine tangutorum and studying the digestion promoting function of the all grass of cardamine tangutorum.

Description

technical field [0001] The invention relates to a method for enriching and purifying total flavonoids of chrysanthemum. Background technique [0002] Cardamine L. is a cruciferous plant. There are about 160 species in the world. They are mainly distributed in temperate regions, where they grow in plains, mountains, sandy land or fine stone land. in calcareous soils. The resource survey shows that: there are 39 kinds of plants of the genus Orina in our country, accounting for about 30% of the total number of Orina genus, and they are distributed in various places in the north and south of my country. The representative plants of the genus are mainly purple-flowered oryza, white-flowered oryza, big-leaf oryza, Chinese central oryza, spring-cracked oryza, paddy-field broken oryza, pot-pot broken oryza, and Viola leaf Broken rice chestnuts, etc. In the prior art, mainly focus on the separation research of the chemical constituents of the broken rice chestnuts in central China...

Claims

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Application Information

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IPC IPC(8): A61K36/31A61P1/14
CPCA61K36/31A61K2236/19A61K2236/333A61K2236/51A61K2236/55A61P1/14
Inventor 刘圆李伯超李莹黄艳菲
Owner SOUTHWEST UNIVERSITY FOR NATIONALITIES
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