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A kind of extraction method of Sorbus nigra and its extract

A technology of Sorbus sagograss and its extraction method, which is applied in the field of Sorbus sagograss extraction method and its extract, can solve the problems of ineffective use of active ingredients of Sorbus sagoculus and complex extraction methods, etc., and achieve high extraction efficiency , Improve the effect of economic value

Active Publication Date: 2021-05-07
北京国康本草物种生物科学技术研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Existing methods for extracting Sorbus nigrum are relatively complicated, and the active ingredients of Sorbus nigrum cannot be effectively utilized

Method used

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  • A kind of extraction method of Sorbus nigra and its extract
  • A kind of extraction method of Sorbus nigra and its extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] A kind of extracting method of Sorbus nigra, the steps are as follows:

[0048] Wash the Sorbus black fruit, add water in the proportion of 0.5ml water per gram of Sorbus black fruit, beat the pulp (time 30s), add ethanol and hydrochloric acid, adjust the volume fraction of ethanol to 70%, adjust the volume fraction of hydrochloric acid to 0.5%, 40 Extract 3 times at ℃ for 1 hour each time, filter, combine the filtrates, concentrate under reduced pressure, and dry to obtain the Sorbus argentina extract rich in polyphenols and anthocyanins;

[0049] Add 5 times the volume of water (v / m) to the filter residue, add 0.1% biological enzyme (pectinase: protease 2:1), enzymolyze at 40°C for 1 hour, filter, add 6 times the volume of water (v / m) to the filter residue , extracted twice at 80°C for 1 hour each time, filtered, combined the filtrates, added ethanol, precipitated polysaccharides, proteins and other substances, filtered, dried the supernatant, and obtained Sorbus nigr...

Embodiment 2

[0051] A kind of extracting method of Sorbus nigra, the steps are as follows:

[0052] Sorbus black fruit, wash, add water in the proportion of 0.5ml water per gram of Sorbus black fruit, beating (time 90s), add ethanol, make the volume fraction be 80%, add 1% hydrochloric acid, extract 3 times at 50 ℃, Filter for 1 hour each time, combine the filtrates, concentrate under reduced pressure, and dry to obtain the Sorbus nigra extract rich in polyphenols and anthocyanins;

[0053] Add 5 times the volume of water (v / m) to the filter residue, add 0.5% biological enzyme (pectinase: cellulase: protease 2:1:1), enzymatically hydrolyze at 40°C for 1 hour, filter, add 6 times the volume of Water (v / m), extract twice at 90°C for 1 hour each time, filter, combine the filtrates, add ethanol, precipitate polysaccharides, proteins and other substances, filter, and dry the supernatant to obtain Sorbus nigra water-soluble dietary fiber.

Embodiment 3

[0055] A kind of extracting method of Sorbus nigra, the steps are as follows:

[0056] Wash the Sorbus black fruit, add water in the proportion of 0.5ml water per gram of Sorbus black fruit, beat the pulp (time 30s), add ethanol and hydrochloric acid, adjust the volume fraction of ethanol to 70%, adjust the volume fraction of hydrochloric acid to 0.5%, 40 Extract 3 times at ℃ for 1 hour each time, filter, combine the filtrates, concentrate under reduced pressure, and dry to obtain the Sorbus argentina extract rich in polyphenols and anthocyanins;

[0057] Add 5 times the volume of water (v / m) to the filter residue, add 0.1% biological enzyme (pectinase: cellulase 4:1), enzymolyze at 40°C for 1 hour, filter, add 6 times the volume of water (v / m) to the filter residue m), extract twice at 80° C. for 1 hour each time, filter, combine the filtrates, add ethanol, precipitate polysaccharides, proteins and other substances, filter, and dry the supernatant to obtain the Sorbus nigra w...

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Abstract

The invention relates to the field of crop extraction, in particular to a method for extracting Sorbus nigra and its extract. A method for extracting Sorbus nigrum, comprising the following steps: beating the fresh fruit of Sorbus nigrum to obtain a Sorbus nigrue slurry; extracting the Sorbus nigrue slurry with ethanol solution and hydrochloric acid solution as solvents to obtain polyphenol-rich, The extract of anthocyanins; the remaining filter residue after extraction is added to the biological enzyme as the first filter residue for enzymolysis, and the obtained enzymolysis liquid is filtered to obtain the filtrate and the second filter residue, and the second filter residue is subjected to water extraction to obtain a water extract; the filtrate and The water extracts are combined, precipitated and separated to obtain an extract mainly composed of polysaccharides and proteins, and the remaining supernatant is a solution containing the water-soluble dietary fiber of Sorbus nigra. The invention realizes high-efficiency extraction of the components of the black-fruited rowan, enables the classified utilization of the components, and improves the economic value of the black-fruited rowan.

Description

technical field [0001] The invention relates to the field of crop extraction, in particular to a method for extracting Sorbus nigra and its extract. Background technique [0002] Aroniamelanocarpa, also known as aroniamelanocarpa, Aronia berry, and old berry, is a deciduous shrub belonging to Rosaceae Aronia. It can be used as medicine and a food with high nutritional value. Nutritional studies have found that the fruit of Sorbus argentina has potential liver-protecting, heart-protecting, blood-sugar-lowering and anti-mutation effects, and has been used in European and American countries to develop functional foods for the prevention of hypertension, cardiovascular and cerebrovascular diseases. At present, there are few related products in the Chinese market. [0003] The patents on the extraction of active ingredients from Sorbus argentina are as follows: [0004] The application number is 201510573488.1, which discloses a production method of Aronia nigra extract. The s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/73A61P39/06A61P9/12A61K131/00
CPCA61K36/73A61K2236/19A61K2236/33A61K2236/331A61K2236/333A61K2236/39A61P9/12A61P39/06
Inventor 王代军闫雪孟王娟科陈松
Owner 北京国康本草物种生物科学技术研究院有限公司
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