Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for lowering detection limit of lateral flow chromatographic test paper

A lateral flow chromatography and test paper technology, applied in the field of rapid detection, can solve the problems that the enrichment and concentration method is not simple enough, reduce the detection limit of the naked eye, and the detection limit is high, so as to increase the detection cost, reduce the detection limit, and have high flexibility. Effect

Active Publication Date: 2018-10-16
JIANGNAN UNIV
View PDF6 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to solve the problems that the detection limit of lateral flow chromatography test paper technology is too high and the existing enrichment and concentration methods are not simple enough, the present invention improves the structure, assembly sequence and sample loading method of lateral flow chromatography test paper, and provides A method for pre-enrichment and sensitization of target substances on lateral flow chromatography test paper is achieved, which is simple and significantly reduces the detection limit of the naked eye

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for lowering detection limit of lateral flow chromatographic test paper
  • Method for lowering detection limit of lateral flow chromatographic test paper
  • Method for lowering detection limit of lateral flow chromatographic test paper

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] A method for reducing the detection limit of lateral flow chromatography test paper (based on a sandwich detection mechanism), the detection target is the DNA sequence 5'-CTGTGCGTGTGACAGCGGCTGA-3' (sequence shown as SEQ ID NO: 1) that simulates the microRNA miR-210, The detection mechanism is similar to the sandwich detection mechanism, including the following steps:

[0044] 1) Synthesis of gold nanoparticles

[0045] Boil 49 mL of ultrapure water and 1 mL of 50 mM aqueous solution of chloroauric acid in a 100 mL round-bottomed flask, then quickly replace the hot electromagnetic stirring heating mantle at the bottom of the round-bottomed flask with a flat-plate electromagnetic stirrer, and stir the mixture as soon as possible while it is still hot. 5mL of 38.8mM trisodium citrate aqueous solution was added to the above round-bottomed flask, the solution in the flask changed from yellow to blue to dark red, and cooled to room temperature with stirring. That is, the gol...

Embodiment 2

[0053] A method for reducing the detection limit of lateral flow chromatography test paper (based on competition detection mechanism), the detection target is the DNA sequence 5'-CTGTGCGTGTGACAGCGGCTGA-3' of the simulated microRNA miR-210 (sequence shown in SEQ ID NO:1) , the detection mechanism is a competitive detection mechanism, including the following steps:

[0054] 1) Synthesis of gold nanoparticles

[0055] Boil 24.5mL ultrapure water and 0.5mL 50mM chloroauric acid aqueous solution in a 50mL round-bottomed flask, then quickly replace the hot electromagnetic stirring heating mantle at the bottom of the round-bottomed flask with a flat-plate electromagnetic stirrer, and stir while hot Add 2.5mL of 38.8mM trisodium citrate aqueous solution into the above-mentioned round bottom flask as soon as possible, the solution in the flask turns from yellow to blue and then to dark red, and cool to room temperature under stirring. That is, the gold nanoparticle hydrosol is obtaine...

Embodiment 3

[0063] A method for reducing the detection limit of lateral flow chromatography test paper (based on sandwich detection mechanism), wherein the detection target is thrombin, comprising the following steps:

[0064] 1) Synthesis of gold nanoparticles

[0065] Boil 98mL of ultrapure water and 2mL of 50mM chloroauric acid aqueous solution in a 250mL round-bottomed flask, then quickly replace the hot electromagnetic stirring heating mantle at the bottom of the round-bottomed flask with a flat-plate electromagnetic stirrer, and stir as soon as possible while hot Add 10mL of 38.8mM trisodium citrate aqueous solution into the above-mentioned round bottom flask, the solution in the flask turns from yellow to blue and then to deep red, and cool to room temperature under stirring. That is, the gold nanoparticle hydrosol is obtained. The above hydrosol was filtered through a 0.45 μm polyethersulfone aqueous filter, and stored as a gold nanoparticle stock solution in a refrigerator at 4 ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for lowering the detection limit of lateral flow chromatographic test paper, and belongs to the technical field of quick detection. The method includes sequentially arranging sample pads, chromatographic films and absorbent pads (without conjugation pads) in a linearly arraying manner; loading sample solution on the sample pads to preliminarily enrich target detection objects on detection zones (T zones) of the chromatographic films; inserting the conjugation pads between the sample pads and the chromatographic films; continuing to load sample solution or buffer solution on the sample pads. Compared with other methods for lowering the detection limit of existing lateral flow chromatographic test paper on the basis of sample enrichment and concentration, themethod has the obvious advantages that the detection limit can be lowered to a great extent; other amplification reagents, materials and equipment can be omitted, the detection cost does not need tobe increased, the method is simple, convenient and speedy and is high in efficiency, and the like.

Description

technical field [0001] The invention relates to a method for reducing the detection limit of lateral flow chromatography test paper, and belongs to the technical field of rapid detection. Background technique [0002] The lateral flow chromatography test strip prepared based on the lateral flow chromatography technology can be used for the rapid detection of small molecules, proteins, nucleic acids and other biological macromolecules and microorganisms on site. The detection method is easy to operate, low in cost, and the detection results are visible to the naked eye, and has been widely used in the fields of clinical diagnosis, environmental monitoring and food safety analysis. At present, the mainstream lateral flow chromatography test paper consists of four parts: the sample pad, the binding pad, the chromatography membrane and the water absorption pad. These four parts are superimposed on the supporting bottom plate to form a lateral flow chromatography test strip. The...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/53G01N33/531
Inventor 严秀平张毅张晓晓杨晗婕曹杭之马孝影黎炎张现童
Owner JIANGNAN UNIV