Intestinal content separation and recycling device and method and product thereof

A technology for separation and recovery of content, applied in the direction of microorganism-based methods, biochemical cleaning devices, biochemical equipment and methods, etc., can solve the problem of the inability to selectively screen intestinal microorganisms, the inability to achieve repeatable operations, and the results Uncontrollable and other problems, to achieve the effect of preventing micropore clogging, high degree of automation, and easy operation

Pending Publication Date: 2018-10-19
QIHUI BIOTECHNOLOGY YANGZHOU CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This collection method is very inconvenient to operate. And due to the limitations of the technology used, many small and light bacteria are poured out with the supernatant, and there are still a large number of fecal residues (more than 40 μm) in the retained species, while Most gut bacteria are smaller than 5 μm in size
Moreover, due to the diversity and complexity of stool samples, and the differences in each person's operation, the method is very different, the results are uncontrollable, and standardized repeatable operations cannot be achieved.
In addition, the fecal bacteria obtained by these methods are mixed, and specific intestinal microorganisms cannot be selectively screened, which is also unsatisfactory for the precise treatment of intestinal diseases in the future

Method used

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  • Intestinal content separation and recycling device and method and product thereof
  • Intestinal content separation and recycling device and method and product thereof
  • Intestinal content separation and recycling device and method and product thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0026] A device for separating and recovering intestinal microorganisms, such as figure 1 As shown, it includes an impurity removal module 1 and a target content collection module 2. The target content collection module includes a microporous membrane filter 5, and the microporous membrane adopts a nuclear pore membrane. The impurity removal module is a filter module, and the filter module includes a stirring coarse filter module 3 and a fine filter module 4. The stirring coarse filter module 3 is a container 7 with a stirring device 6 on the top, and the bottom of the container 7 is provided with a coarse filter screen 8. The aperture of filter screen 8 is 2mm, after 130 grams of feces and 750ml normal saline are mixed in container 7, be fully dispersed by agitator 6, remove the larger impurity in the feces through coarse filter screen 8 filtration, fine filter module 4 It includes two layers of fine filter screens 9 with different apertures. The apertures of the fine filter ...

Embodiment 2

[0034] A device for separating and recovering intestinal microorganisms, such as Figure 4 As shown, it includes an impurity removal module 1 and a target content collection module 2. The target content collection module includes a microporous membrane filter 5, and the microporous membrane adopts a nuclear pore membrane. The impurity removal module is a filter module, and the filter module includes a stirring coarse filter module 3 and a fine filter module 4. The stirring coarse filter module 3 is a container 7 with a stirring device 6 on the top, and the bottom of the container 7 is provided with a coarse filter screen 8. The aperture of filter screen 8 is 2mm, after 130 grams of feces and 750ml normal saline are mixed in container 7, be fully dispersed by agitator 6, remove the larger impurity in the feces through coarse filter screen 8 filtration, fine filter module 4 It includes two layers of fine filter screens 9 with different apertures. The apertures of the fine filter...

Embodiment 3

[0039] A device for separating and recovering intestinal microorganisms, such as Figure 5 As shown, it includes an impurity removal module 1 and a target content collection module 2. The target content collection module includes a microporous membrane filter 5, and the microporous membrane adopts a nuclear pore membrane. The impurity removal module is a filter module, and the filter module includes a stirring coarse filter module 3 and a fine filter module 4. The stirring coarse filter module 3 is a container 7 with a stirring device 6 on the top, and the bottom of the container 7 is provided with a coarse filter screen 8. The aperture of filter screen 8 is 2mm, after 130 grams of feces and 750ml normal saline are mixed in container 7, be fully dispersed by agitator 6, remove the larger impurity in the feces through coarse filter screen 8 filtration, fine filter module 4 It includes two layers of fine filter screens 9 with different apertures. The apertures of the fine filter...

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Abstract

The invention relates to an intestinal content separation and recycling device and method and a product thereof. The device comprises an impurity removal module 1 and a target content collection module 2, wherein the target content collection module comprises a micro-pore membrane filtering net 5; after excrement is mixed with a solvent (such as normal saline), impurities are firstly removed by utilizing the impurity removal module 1, filtration is implemented by utilizing the micro-pore membrane filtering net 5 secondly, and target contents are collected from the micro-pore membrane filteringnet 5. By utilizing a micro-pore membrane technique, intestinal microorganisms are separated and extracted, a conventional centrifugal separation technique is replaced, firstly, an extraction processcan be precise and controllable, high in separation efficiency and meanwhile simple, convenient to operate and high in automation degree, secondly, micro-pore membranes of different apertures can beused, due to a size effect, expected intestinal microorganisms can be selectively separated, and relative abundance of different bacteria can be precisely controlled.

Description

technical field [0001] The invention belongs to the technical field of medical devices, in particular to the technical field of separation of intestinal contents. Background technique [0002] Human intestinal microbes are always accompanied by human growth, not only helping to digest food, but also an effective barrier for the human body to resist the invasion of external pathogenic bacteria. The balanced and stable intestinal microecological environment formed by the long evolutionary process plays a vital role in the nutrition, metabolism and immunity of the human body. There are a large number of symbiotic microorganisms in the human body, most of which live in the human intestinal tract, and the number exceeds 1000 trillion (10 14 Order of magnitude), more than 10 times the total number of human cells. In the long evolutionary process, intestinal microbes have reached a good cooperation with human beings, and play a vital role in the nutrition, metabolism and immunity...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/26C12M1/12C12M1/02C12M1/00C12N1/02C12R1/01C12R1/19C12R1/225
CPCC12M27/02C12M23/48C12M29/06C12M33/14C12N1/02
Inventor 勾振辉骆奇
Owner QIHUI BIOTECHNOLOGY YANGZHOU CO LTD
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