Uses of chlorogenic acid in preparation of injections with skin whitening effect
A technology of skin whitening and injection, which is applied in the field of medicine and can solve problems such as increasing melanin content
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Embodiment 1
[0023] Preparation of Example 1 Chlorogenic Acid Freeze-dried Powder for Injection
[0024] In this example, chlorogenic acid freeze-dried powder for injection was prepared according to the method of Example 1 in patent ZL201310366945.0.
[0025] 1. Main drug
[0026] Chlorogenic acid, extracted from Eucommia ulmoides leaves by Sichuan Jiuzhang Biotechnology Co., Ltd., has a purity of 98.4%.
[0027] 2. Prescription:
[0028]
[0029] According to the above prescription, 1000 chlorogenic acid freeze-dried powders for injection were prepared by freeze-drying with a specification of 30 mg / bottle.
[0030] In the following experimental examples, the chlorogenic acid freeze-dried powder injections were all prepared according to Example 1.
[0031] The beneficial effects of the present invention are demonstrated through experimental examples below.
experiment example 1
[0032] Experimental Example 1 Clinical trial of chlorogenic acid for injection to improve facial skin pigmentation and skin whitening effect
[0033] There is currently a phase I clinical trial of chlorogenic acid for injection in the treatment of malignant glioma (clinical approval number: 2013L01855) carried out in Beijing Shijitan Hospital Affiliated to Capital Medical University. The administration dose of the group is respectively intramuscular injection of chlorogenic acid freeze-dried powder injection (prepared according to Example 1) every morning, and continuous injection for 4 weeks.
[0034] As an exploratory index of clinical trials, after using chlorogenic acid for injection, all subjects were ① using computer local skin photography to observe the change of skin color; ② testing their skin pigment in MDD-MX18 skin melanin tester, There are 12 subjects in total.
[0035] Among them, computer partial photos were used to compare the changes in skin color. The curat...
experiment example 2
[0042] Experimental Example 2 Animal Research of Inhibiting Pigment Synthesis with Chlorogenic Acid for Injection
[0043] 1. Experimental drugs and animals
[0044] Chlorogenic acid freeze-dried powder for injection was provided by Sichuan Jiuzhang Biotechnology Co., Ltd.; pure chlorogenic acid with a purity of 99.5% was provided by Sichuan Jiuzhang Biotechnology Co., Ltd.; Balb / c mice were purchased from Sichuan Provincial People’s Hospital.
[0045] 2. Grouping of experimental animals and experimental methods
[0046] The experimental mice that passed the quarantine were randomly divided into 4 groups, 10 in each group: ① Group 1 was the blank control group; ② Group 2 was the intramuscular administration group of chlorogenic acid for injection; ③ Group 3 was the intravenous administration of chlorogenic acid for injection group; ④ Group 4 was oral administration of pure chlorogenic acid;
[0047] Among them, the mice in the blank control group were kept without any treat...
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