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Application of the protein nog1 in the regulation of plant yield and grain number per panicle

A protein and grain number per panicle technology, applied in the biological field, can solve problems such as narrow genetic diversity, similar genetic basis and genotype, and constraints on rice yield potential

Active Publication Date: 2019-12-13
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The researchers believe that many cultivars currently used in production have the same or similar genetic sources, and the utilization of genetic resources of rice cultivars tends to be saturated. The narrow genetic diversity among rice varieties has caused the genetic basis and genotype of rice varieties Similarly, this has become a bottleneck restricting the further improvement of rice yield potential

Method used

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  • Application of the protein nog1 in the regulation of plant yield and grain number per panicle
  • Application of the protein nog1 in the regulation of plant yield and grain number per panicle
  • Application of the protein nog1 in the regulation of plant yield and grain number per panicle

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Embodiment 1, the discovery of nog1 gene

[0063] Using Jiangxi Dongxiang wild rice as the donor parent and Guichao 2 as the recurrent parent, a set of introgressive line populations containing 265 lines (one of which is SIL176) was constructed by crossing and backcrossing. The coverage rate of the wild rice genome in this population is 79.4%, and the yield per plant of 15 lines (one of which is SIL176) is more than 35% lower than that of Guichao 2. The morphology of the main stem panicle, the number of grains on the main stem panicle and the yield per plant of Guichao 2 and SIL176 were compared and counted. The experiment was repeated three times, with 30 plants each time.

[0064] See the experimental results figure 1 (A is the shape of the main stem and ear, bar=5cm; B is the number of grains on the main stem and ear; C is the yield per plant; ** indicates that P<0.01 is extremely significant). The results showed that compared with Guichao 2, the number of grains...

Embodiment 2

[0066] Example 2, T 2 Obtaining and phenotypic identification of homozygous RNAi interfering strains

[0067] 1. Construction of recombinant plasmid pRNAi-nog1

[0068] The construction steps of the recombinant plasmid pRNAi-nog1 are as follows:

[0069] 1. Synthetic primers

[0070] According to the sequence of the nog1 gene shown in Sequence 1 in the sequence listing, primers 860-rnai-320F, 860-rnai-681R, 860-rnai-681F and 860-rnai-314R were designed and synthesized; the primer sequences are as follows:

[0071] 860-rnai-320F: 5′-GG ACTAGT GGGAGAAAGATGAGGA-3' (the underline is the recognition site of restriction endonuclease SpeI);

[0072] 860-rnai-681R: 5′-TCC GAGCTC GGTCAAAGCCAGGTAC-3' (the underline is the restriction endonuclease SacI recognition site);

[0073] 860-rnai-681F: 5′-CG GGATCC GGTCAAAGCCAGGTAC-3' (the underline is the restriction endonuclease BamHI recognition site);

[0074] 860-rnai-314R: 5′-GG GGTAC CAGAGCTGGGAGAAAGA-3' (the underline is the...

Embodiment 3

[0103] Example 3, T 2 Generation and phenotypic identification of homozygous complementation lines

[0104] 1. Construction of recombinant plasmid pCAMBIA1300-NOG1

[0105] The construction steps of the recombinant plasmid pCAMBIA1300-NOG1 are as follows:

[0106] 1. Using the 2-week-old seedling experimental material of Guichao 2, extract genomic DNA and use it as a template, use 860HBF: 5'-GA AGATCT CATCTGATGCCTCATACTGA-3' (the underline is the restriction endonuclease BglII recognition site) and 860HBR: 5'-CCG ACGCGT CATGCTTAGGCTGTTGAT-3' (the underline is the recognition site of the restriction endonuclease MluI) was used as a primer for PCR amplification to obtain a PCR amplification product of about 7 kb.

[0107] 2. Digest the PCR amplification product with restriction endonucleases BglII and MluI, and recover the digested product.

[0108] 3. The transformed plant expression vector pCAMBIA1300 was digested with restriction endonucleases BglII and MluI, and about ...

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Abstract

The invention discloses the application of protein nog1 in regulating plant yield and / or grain number per panicle. The amino acid sequence of the protein nog1 is the protein shown in Sequence 2 in the sequence table; the yield is the yield per plant; the number of grains per spike is the number of spikes on the main stem. Experiments have shown that transgenic plant B is obtained by introducing a substance that inhibits the expression of the protein nog1 into Guichao 2; compared with Guichao 2, the yield per plant of transgenic plant B is reduced and / or the number of grains per spike on the main stem is reduced. The nucleic acid molecule encoding the protein nog1 is introduced into SIL176 to obtain transgenic plant A; compared with SIL176, the yield per plant of the transgenic plant A increases and / or the number of grains on the main stem increases. Therefore, the protein nog1 plays a very important role in the regulation of rice yield and grain number per panicle.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of protein nog1 in regulating plant yield and grain number per spike. Background technique [0002] Rice is one of the most important food crops in the world. More than 120 countries around the world grow rice, and the cultivated area is maintained at more than 150 million hectares all year round. 50% of the world's population uses rice as a staple food. Today, as the population continues to increase and the area of ​​arable land that can be planted decreases year by year, increasing rice yield per unit area is one of the powerful measures to ensure world food security. Looking back on the history of rice breeding for more than half a century, my country's rice yield has experienced two leaps, the first is the green revolution marked by dwarf breeding, and the second is the utilization of rice heterosis. But rice yields have stagnated for nearly 20 years. The resear...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/82
CPCC12N15/8218C12N15/8261C07K14/415Y02A40/146A01H5/10
Inventor 孙传清霍兴谭禄宾刘凤霞付永彩朱作峰顾凭
Owner CHINA AGRI UNIV
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