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Preparation method of xylooligosaccharide

A technology of xylooligosaccharides and strains, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of high production cost, low conversion efficiency, high equipment requirements, etc., and achieve high yield and production Simple process and high efficiency

Inactive Publication Date: 2018-10-26
JUNHE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the conversion efficiency of these methods is low, the chemical pretreatment process is complicated, the equipment requirements are high, and the sewage treatment pressure is high, resulting in high production costs.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Crush the corn cob until the particle size is less than or equal to 2mm, take 100 grams of corn cob powder, add 900 grams of water, cook at 125 degrees Celsius for 1 hour to sterilize, add 5 grams of yeast extract after sterilization, and then insert Clostridium kleifrey (Clostridium clariflavum) bacterium liquid 10 grams, temperature is controlled at 60 degrees centigrade, pH is controlled at 7, hydrolytic fermentation is 5 days. The total conversion rate of cellulose and hemicellulose after hydrolysis and fermentation is 75%. The product is 55 grams, and the product contains xylooligosaccharides, amino acids, acetic acid, lactic acid, ethanol, etc., wherein 25 grams of xylooligosaccharides.

Embodiment 2

[0042] Crush wheat straw until the particle size is less than or equal to 2mm, take 100 grams of wheat straw powder, add 900 grams of water, cook at 125 degrees Celsius for 1 hour to sterilize, add 5 grams of yeast extract after sterilization, and then inoculate Clostridium kleiferi (Clostridium clariflavum) bacterial liquid 10 grams, temperature is controlled at 60 degrees centigrade, pH is controlled at 7, hydrolytic fermentation is 5 days. The total conversion rate of cellulose and hemicellulose after hydrolysis and fermentation is 70%. The product is 45 grams, and the product contains xylooligosaccharides, amino acids, acetic acid, lactic acid, ethanol, etc., wherein, 17 grams of xylooligosaccharides.

Embodiment 3

[0044] Crush corncobs until the particle size is less than or equal to 2mm, take 100 grams of corncob powder, add 900 grams of water, cook at 125 degrees Celsius for 1 hour to sterilize, add 5 grams of yeast extract after sterilization, and then insert Clostridium thermocellum ) 10 grams of bacterial liquid, the temperature is controlled at 60 degrees Celsius, the pH is controlled at 7, and hydrolysis and fermentation are carried out for 5 days. The total conversion rate of cellulose and hemicellulose after hydrolysis and fermentation is 75%. The product is 55 grams, and the product contains xylooligosaccharides, amino acids, acetic acid, lactic acid, ethanol, etc., wherein xylooligosaccharides are 25 grams.

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PUM

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Abstract

The invention relates to a preparation method of xylooligosaccharide. The xylooligosaccharide is prepared by hydrolysis fermentation of lignocellulosic biomass materials from one or multiple strains of self-produced cellulase and / or hemicellulase. The high effective strains are adopted, enzyme is hydrolyzed by the aid of the self-secreted cellulase and hemicellulase of the strains, the lignocellulosic materials are degraded by controllable liquid deep hydrolysis fermentation, and reparation of the xylooligosaccharide is achieved; compared with the prior art, the preparation method has the advantages of wide material application range, high yield, simple production process and high efficiency.

Description

technical field [0001] The invention relates to a preparation method of xylooligosaccharide. Background technique [0002] Xylo-oligosaccharide (Xylo-oligosaccharide) is an oligosaccharide mixture composed of 2-7 D-xylose residues combined with β-1,4 glycosidic bonds. Xylo-oligosaccharide is one of the varieties with the strongest function of proliferating bifidobacteria among polymeric sugars. Its efficacy is nearly 20 times that of other polymeric sugars. There is no enzyme to hydrolyze xylo-oligosaccharide in the human gastrointestinal tract, so its It can directly enter the large intestine and be preferentially used by bifidobacteria to promote the proliferation of bifidobacteria and produce a variety of organic acids. Lower intestinal pH, inhibit the growth of harmful bacteria, and make probiotics proliferate in large numbers in the intestinal tract. The specific health effects of xylo-oligosaccharides on the human body are: 1. Reduce the production of toxic fermentat...

Claims

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Application Information

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IPC IPC(8): C12P19/00C12P19/04C12P19/12C12R1/145C12R1/01
CPCC12P19/00C12P19/04C12P19/12
Inventor 邵雄俊虞方磊黄永和
Owner JUNHE BIOTECH CO LTD
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