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A carbendazim-degrading bacterium and its application

A technology of bacilli and strains, applied in the field of carbendazim-degrading bacteria, can solve the problems of no public reports on the application of microbial degradation of pesticide residues, and achieve the effect of degrading carbendazim pesticide residues and low cost of use

Active Publication Date: 2021-06-08
YUNNAN UNIV
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  • Application Information

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Problems solved by technology

Sphingobacterium nematocida M-SX103 strain is a new bacterial species reported by the inventors in 2012 (Liu et al., 2012). In recent studies, the inventors first discovered that the M-SX103 strain has high-efficiency degradation ability to carbendazim , the effect of degrading carbendazim residues on tobacco leaves is remarkable; however, there are no public reports on the characteristics of this microorganism in degrading pesticide residues and its application in tobacco planting

Method used

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  • A carbendazim-degrading bacterium and its application
  • A carbendazim-degrading bacterium and its application
  • A carbendazim-degrading bacterium and its application

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preparation example Construction

[0023] Preparation of M-SX103 wettable powder: the microbial cells and their metabolites obtained by fermenter culture are mixed with an appropriate amount of diatomaceous earth, dried at below 65°C or air-dried to a moisture content of less than 5%, and pulverized to make . It is necessary to ensure that the viable count of the M-SX103 strain in the inoculum is 1×10 9 pcs / gram or more.

[0024] 2. Degradation effect test of bacterial agent of the present invention to residues of carbendazim in field tobacco leaves

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Abstract

The invention relates to a carbendazim-degrading bacterium and its application, and belongs to the technical field of microbial degradation of chemical pesticide residues. The carbendazim-degrading bacterium is Sphingobacterium nematocida M‑SX103 strain, which has been preserved in the General Microorganism Center of China Committee for the Collection of Microbial Cultures on September 18, 2014; the address of the depository unit: Beijing Institute of Microbiology, Chinese Academy of Sciences, No. 3, Courtyard No. 1, Beichen West Road, Chaoyang District, City; collection number: CGMCC No.9689. Application of M-SX103 strain in the preparation of bacterial agent for degrading tobacco leaf and tobacco planting soil multibacterial pesticide residues in tobacco cultivation. The beneficial effect of the present invention is that: the bacterial agent prepared by using the M-SX103 strain can efficiently degrade the carbendazim pesticide residue in tobacco planting, and has the characteristics of low use cost, no residue, and safety to humans and animals.

Description

Technical field: [0001] The invention relates to a carbendazim-degrading bacterium and application thereof, and belongs to the technical field of microbial degradation of chemical pesticide residues. Background technique: [0002] Carbendazim belongs to the main pesticides in benzimidazole fungicides. Benzimidazole fungicides have the characteristics of strong systemic absorption, high efficiency, broad spectrum and low toxicity, and have strong bactericidal effect on plant pathogenic fungi. It is widely used in the control of fungal diseases of various crops such as vegetables and fruits. In China, although carbendazim is not registered for the prevention and control of tobacco diseases, carbendazim is a commonly used pesticide in agricultural production. Anthrax, scab and low blackhead disease. At the same time, thiophanate-methyl, which is registered for the prevention and control of tobacco root black rot, can also be converted into carbendazim in tobacco plants, and t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A62D3/02C12R1/01A62D101/04A62D101/26
CPCA62D3/02A62D2101/04A62D2101/26C12N1/205C12R2001/01
Inventor 莫明和祝明亮夏振远刘子仪马莉
Owner YUNNAN UNIV
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