High-yield extraction method for polysaccharide of lotus seeds
An extraction method and high-yield technology are applied in the field of high-yield extraction of lotus seed polysaccharide, can solve the problems of low polysaccharide yield, yield and purity, and achieve the effect of convenience of microcrystalline cellulose
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Embodiment 1
[0031] A method for extracting lotus seed polysaccharide with high yield, comprising the following steps:
[0032] A, pretreatment: after washing the lotus seeds, add microcrystalline cellulose to coarsely pulverize them, put them into an ultrafine pulverizer for ultrafine pulverization after coarse pulverization, and obtain lotus seed fine powder;
[0033] B. Emulsification: Put the lotus seed powder into water, add emulsifier Tween-80 and fully emulsify to obtain the lotus seed emulsion containing microcrystalline cellulose;
[0034] C, filtration: the lotus seed emulsion is filtered to obtain filtrate and filter residue;
[0035] D. Filter residue treatment: 1) Alkali foaming: soak the filter residue in step C in alkaline solution to obtain soaking material liquid, 2) Neutralization: use acid to neutralize the soaking material liquid, adjust the pH to 7.5, 3) once Filtration: filter the neutralized soaking liquid to obtain filtrate and filter residue, 4) Secondary filtrati...
Embodiment 2
[0041] A method for extracting lotus seed polysaccharide with high yield, comprising the following steps:
[0042] A. Pretreatment: Wash the lotus seeds and dry them at 30°C. The moisture content of the dried lotus seeds is 10%. Then add microcrystalline cellulose to the dried lotus seeds for coarse crushing. Put it into a superfine pulverizer for superfine pulverization to obtain lotus seed powder;
[0043] B. Emulsification: Put the lotus seed powder into water, add emulsifier Tween-80 and fully emulsify to obtain the lotus seed emulsion containing microcrystalline cellulose;
[0044] C, filtration: the lotus seed emulsion is filtered to obtain filtrate and filter residue;
[0045] D. Filter residue treatment: 1) Alkali foaming: soak the filter residue in step C in alkali solution to obtain soaking material liquid, 2) neutralization: use acid to neutralize the soaking material liquid, adjust the pH to 6.5, 3) once Filtration: filter the neutralized soaking liquid to obtain...
Embodiment 3
[0051] A method for extracting lotus seed polysaccharide with high yield, comprising the following steps:
[0052] A. Pretreatment: Wash the lotus seeds and dry them at 40°C. The moisture content of the dried lotus seeds is 5%. Then add microcrystalline cellulose to the dried lotus seeds for coarse crushing. Freeze at -8°C for 2 hours, and then put it into a superfine pulverizer for superfine pulverization to obtain lotus seed powder;
[0053] B. Emulsification: Put the lotus seed powder into water, add emulsifier Tween-80 and fully emulsify to obtain the lotus seed emulsion containing microcrystalline cellulose;
[0054] C, filtration: the lotus seed emulsion is filtered to obtain filtrate and filter residue;
[0055] D. Treatment of filter residue: 1) Alkali foaming: soak the filter residue in step C in alkaline solution to obtain soaking liquid, 2) neutralization: use acid to neutralize the soaking liquid, adjust the pH to 7, 3) once Filtration: filter the neutralized soa...
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