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High-yield extraction method for polysaccharide of lotus seeds

An extraction method and high-yield technology are applied in the field of high-yield extraction of lotus seed polysaccharide, can solve the problems of low polysaccharide yield, yield and purity, and achieve the effect of convenience of microcrystalline cellulose

Active Publication Date: 2018-11-06
福建恒正品食品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the polysaccharide yield, yield and purity extracted by this method are not high.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A method for extracting lotus seed polysaccharide with high yield, comprising the following steps:

[0032] A, pretreatment: after washing the lotus seeds, add microcrystalline cellulose to coarsely pulverize them, put them into an ultrafine pulverizer for ultrafine pulverization after coarse pulverization, and obtain lotus seed fine powder;

[0033] B. Emulsification: Put the lotus seed powder into water, add emulsifier Tween-80 and fully emulsify to obtain the lotus seed emulsion containing microcrystalline cellulose;

[0034] C, filtration: the lotus seed emulsion is filtered to obtain filtrate and filter residue;

[0035] D. Filter residue treatment: 1) Alkali foaming: soak the filter residue in step C in alkaline solution to obtain soaking material liquid, 2) Neutralization: use acid to neutralize the soaking material liquid, adjust the pH to 7.5, 3) once Filtration: filter the neutralized soaking liquid to obtain filtrate and filter residue, 4) Secondary filtrati...

Embodiment 2

[0041] A method for extracting lotus seed polysaccharide with high yield, comprising the following steps:

[0042] A. Pretreatment: Wash the lotus seeds and dry them at 30°C. The moisture content of the dried lotus seeds is 10%. Then add microcrystalline cellulose to the dried lotus seeds for coarse crushing. Put it into a superfine pulverizer for superfine pulverization to obtain lotus seed powder;

[0043] B. Emulsification: Put the lotus seed powder into water, add emulsifier Tween-80 and fully emulsify to obtain the lotus seed emulsion containing microcrystalline cellulose;

[0044] C, filtration: the lotus seed emulsion is filtered to obtain filtrate and filter residue;

[0045] D. Filter residue treatment: 1) Alkali foaming: soak the filter residue in step C in alkali solution to obtain soaking material liquid, 2) neutralization: use acid to neutralize the soaking material liquid, adjust the pH to 6.5, 3) once Filtration: filter the neutralized soaking liquid to obtain...

Embodiment 3

[0051] A method for extracting lotus seed polysaccharide with high yield, comprising the following steps:

[0052] A. Pretreatment: Wash the lotus seeds and dry them at 40°C. The moisture content of the dried lotus seeds is 5%. Then add microcrystalline cellulose to the dried lotus seeds for coarse crushing. Freeze at -8°C for 2 hours, and then put it into a superfine pulverizer for superfine pulverization to obtain lotus seed powder;

[0053] B. Emulsification: Put the lotus seed powder into water, add emulsifier Tween-80 and fully emulsify to obtain the lotus seed emulsion containing microcrystalline cellulose;

[0054] C, filtration: the lotus seed emulsion is filtered to obtain filtrate and filter residue;

[0055] D. Treatment of filter residue: 1) Alkali foaming: soak the filter residue in step C in alkaline solution to obtain soaking liquid, 2) neutralization: use acid to neutralize the soaking liquid, adjust the pH to 7, 3) once Filtration: filter the neutralized soa...

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PUM

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Abstract

The invention discloses a high-yield extraction method for polysaccharide of lotus seeds, and relates to the technical field of polysaccharide of lotus seeds. The high-yield extraction method comprises the following steps: after cleaning the lotus seeds, adding microcrystalline cellulose to carry out coarse grinding, superfine grinding, emulsification and filtration to obtain filter liquor and a filter residue, carrying out centrifugal emulsification on the filter liquor to obtain a supernate and a precipitate, and carrying out different extraction manners on the filter residue, the supernateand the precipitate to obtain the polysaccharide of lotus seeds. The extraction rate of the polysaccharide of lotus seeds is improved by using the microcrystalline cellulose, different extraction manners are carried out on different parts after smashing filtration and centrifugal emulsification according to different lotus seeds, the yield and productivity of the polysaccharide can be improved, and the purity of the polysaccharide can also be improved.

Description

technical field [0001] The invention relates to the technical field of lotus seed polysaccharide extraction, in particular to a high-yield extraction method of lotus seed polysaccharide. Background technique [0002] Polysaccharides are formed by the condensation and dehydration of multiple monosaccharide molecules, and are a class of carbohydrates with complex and large molecular structures. All carbohydrates and their derivatives that conform to the concept of polymer compounds are called polysaccharides. Polysaccharides are widely distributed in nature and are also very important. Some are components that make up the cell walls of animals and plants, such as peptidoglycan and cellulose; some are nutrients stored in animals and plants, such as glycogen and starch; some have special biological activities, such as heparin in the human body, which has anticoagulant effect, Polysaccharides in the cell wall of pneumococci act as antigens. The structural unit of polysaccharid...

Claims

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Application Information

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IPC IPC(8): C12P19/14C12P19/04C08B37/00
CPCC08B37/0003C12P19/04C12P19/14
Inventor 邓加聪李莉娟陈联邦童秋霞
Owner 福建恒正品食品有限公司
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