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Method for preparing lactobacillus plantarum ZJ316 bacteriocin through spray drying

A Lactobacillus plantarum, spray-drying technology, applied in the biological field, can solve the problems of unsatisfactory purification effect, yield and vitality, differences in bacteriocin purification methods, difficult transportation, etc., to avoid dust flying, improve product purity, The effect of ensuring hygienic conditions

Inactive Publication Date: 2018-11-06
ZHEJIANG GONGSHANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the preservation of bacteriocins, in order to avoid the reduction of their activity, laboratories often use the method of freeze-drying, but this method is high in cost, less in handling capacity, difficult in transportation, and is not suitable for large-scale industrial production of bacteriocins
According to the characteristics of different bacteriocins, there are differences in the purification methods of bacteriocins, and the purification effect, yield and activity of the traditional methods disclosed at present are not very ideal

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Lactobacillus plantarum ZJ316 was activated, and the seed liquid that had been subcultured twice was put into the fermenter with an inoculation amount of 3%. During the fermentation process, the stirring speed was 100 r / min, the culture temperature was 30°C, and the fermentation started for 12 h. Start to feed according to the exponential feeding method, calculate the flow acceleration rate through the fed-batch equation to feed, and finally obtain the fermentation broth; filter the fermentation broth through a biofilm filtration system model Sartorius 16828, and collect the supernatant liquid; add salt to the supernatant at a volume ratio of 1:4.8; after mixing, collect through a micro spray dryer model Buchi B-290, set the inlet temperature of the micro spray dryer to 153 °C, and the compressed gas flow rate to 900 L / h, the working efficiency of the peristaltic pump is 18%, and finally the bacteriocin is obtained.

Embodiment 2

[0029] Lactobacillus plantarum ZJ316 was activated, and the seed liquid that had been subcultured twice was put into the fermenter with an inoculation amount of 3%. During the fermentation process, the stirring speed was 100 r / min, the culture temperature was 30°C, and the fermentation started for 12 h. Start to feed according to the exponential feeding method, calculate the flow acceleration rate through the fed-batch equation to feed, and finally obtain the fermentation broth; filter the fermentation broth through a biofilm filtration system model Sartorius 16828, and collect the supernatant liquid; add salt to the supernatant at a volume ratio of 1:5; after mixing, collect through a micro spray dryer model Buchi B-290, set the inlet temperature of the micro spray dryer to 155 °C, and the compressed gas flow rate to 950 L / h, the working efficiency of the peristaltic pump is 20%, and finally the bacteriocin is obtained.

Embodiment 3

[0031]Lactobacillus plantarum ZJ316 was activated, and the seed liquid that had been subcultured twice was put into the fermenter with an inoculation amount of 3%. During the fermentation process, the stirring speed was 100 r / min, the culture temperature was 30°C, and the fermentation started for 12 h. Start to feed according to the exponential feeding method, calculate the flow acceleration rate through the fed-batch equation to feed, and finally obtain the fermentation broth; filter the fermentation broth through a biofilm filtration system model Sartorius 16828, and collect the supernatant liquid; add salt to the supernatant at a volume ratio of 1:5.2; after mixing, collect it through a micro spray dryer model Buchi B-290, set the inlet temperature of the micro spray dryer to 157 °C, and the compressed gas flow rate to 1000 L / h, the working efficiency of the peristaltic pump is 22%, and finally the bacteriocin is obtained.

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Abstract

The invention discloses a method for preparing lactobacillus plantarum ZJ316 bacteriocin through spray drying. The method comprises the following steps: (1), fermenting: activating lactobacillus plantarum ZJ316, and performing fermentation culture to obtain a fermentation solution; (2), separating: filtering the fermentation solution through a membrane separation system, and collecting a supernatant; (3), collecting: adding auxiliary materials into the supernatant, mixing, and then collect by a spray drying method to obtain the bacteriocin. By the method, the yield of the bacteriocin is greatly increased, the vitality of the bacteriocin is greatly improved, and the purity of the bacteriocin is also increased; a liquid state is changed into a solid state, so that the bacteriocin is convenient to store and transport; the liquid-state bacteriocin is unstable, so that after the bacteriocin is changed into the solid state, the biological properties of the bacteriocin are also effectively protected; the defect of high cost of the conventional collecting method is avoided, and the yield of the bacteriocin is effectively increased.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for preparing Lactobacillus plantarum ZJ316 bacteriocin by spray drying. Background technique [0002] Bacteriocin is a short antibacterial peptide produced by Lactobacillus, which is high temperature resistant, acid resistant, degradable in the human body, safe and non-toxic, and has strong antibacterial activity. Bacteriocin is a kind of protein or low molecular weight polypeptide substance with antibacterial activity produced by microorganisms through the ribosome synthesis mechanism. The antibacterial range is not limited to homologous bacteria, and the producing bacteria are autoimmune to their bacteriocins. [0003] As a special antibacterial and antibacterial protein, bacteriocin has the characteristics of strong antibacterial activity, good biocompatibility, stable performance, biodegradability, digestibility and high biological safety. It is used in the prevention a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/02C07K1/34C12R1/25
CPCC07K14/335
Inventor 顾青郦萍王月姣周青青叶开
Owner ZHEJIANG GONGSHANG UNIVERSITY
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