A method for acid-assisted dispersion liquid-liquid micro-extraction of mycomycin in fruit juice

An auxiliary dispersion and fungomycin technology, applied in the field of detection, can solve the problems of high price and high experimental cost, and achieve the effects of high accuracy, simple operation and good controllability of conditions.

Active Publication Date: 2020-11-03
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are four commonly used mycomycin detection methods in the laboratory. Simultaneous detection of multiple mycomycins is mainly to directly extract the mycotoxins in the sample and use liquid chromatography-mass spectrometry for simultaneous determination. However, this method needs to add Full-carbon-labeled stable isotope mycin is used as an internal standard, but it is expensive and the cost of the experiment is high. Now there is a need for an extraction method that can shorten the extraction time, improve detection efficiency, reduce costs, and improve safety.

Method used

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  • A method for acid-assisted dispersion liquid-liquid micro-extraction of mycomycin in fruit juice
  • A method for acid-assisted dispersion liquid-liquid micro-extraction of mycomycin in fruit juice
  • A method for acid-assisted dispersion liquid-liquid micro-extraction of mycomycin in fruit juice

Examples

Experimental program
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Effect test

Embodiment 1

[0024] Add 5 mL of water into a 10 mL stoppered centrifuge tube as a matrix, and add 10 ng / mL of a mixture of 10 target toxins. Prepare 1 mL of hydrochloric acid solution with a pH of 0.5, and add 100 μL o-anisidine as an extractant to dissolve it. Add the prepared mixed solution of hydrochloric acid into water, and then add Na3PO4 buffer solution until no more misty colloids are produced, and then place it in a centrifuge and centrifuge at 4000r / min for 5min.

[0025] Sampling method: Remove the upper aqueous phase solution, use 180 μL of methanol-acetonitrile mixed solution for the sedimentary phase, dilute to 200 μL, dissolve and inject the sample through the membrane.

Embodiment 2

[0027] Add 5 mL of water into a 10 mL stoppered centrifuge tube as a matrix, and add 10 ng / mL of a mixture of 10 target toxins. Prepare 1 mL of hydrochloric acid solution with a pH of 0.5, and add 100 μL of o-toluidine as an extractant to dissolve it. Add the prepared hydrochloric acid mixed solution into water, then add Na 3 PO 4 Buffer solution, until no excess mist colloid is produced, place in a centrifuge and centrifuge at 4000r / min for 5min.

[0028] Sampling method: Remove the upper aqueous phase solution, use 180 μL of methanol-acetonitrile mixed solution for the sedimentary phase, dilute to 200 μL, dissolve and inject the sample through the membrane.

Embodiment 3

[0030] Add 5 mL of water into a 10 mL stoppered centrifuge tube as a matrix, and add 10 ng / mL of a mixture of 10 target toxins. Prepare 1 mL of hydrochloric acid solution with a pH of 0.5, and add 100 μL of trichloroaniline as an extractant to dissolve it. Add the prepared hydrochloric acid mixed solution into water, then add Na 3 PO 4 Buffer solution, until no excess mist colloid is produced, place in a centrifuge and centrifuge at 4000r / min for 5min.

[0031] Sampling method: Remove the upper aqueous phase solution, use 180 μL of methanol-acetonitrile mixed solution for the sedimentary phase, dilute to 200 μL, dissolve and inject the sample through the membrane.

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Abstract

The invention discloses a method for extracting fungimycin in juice by acid-assisted dispersive liquid-liquid microextraction. The method comprises the following steps: adding water to a centrifugal tube as a matrix, and adding 10 ng / mL of a target toxin mixture; preparing hydrochloric acid solution, adding an extraction agent to the hydrochloric acid solution, and preparing hydrochloric acid mixed solution; adding the hydrochloric acid mixed solution to the water, and adding 0.5 mol / L-1Na3PO4 buffer solution and adjusting a pH value, forming vaporific colloid, and centrifuging; and measuringa volume of lower-layer liquid, and taking the lower-layer liquid for detecting. The method is capable of rapidly achieving extraction balance without operations of ultrasound, vortex and the like, and the method is simple.

Description

technical field [0001] The invention relates to the technical field of detection, in particular to a method for acid-assisted dispersion liquid-liquid micro-extraction of mycomycin in fruit juice. Background technique [0002] Mycomycin is a secondary metabolite produced by fungi. It has been present in food, vegetables, and fruits for many years, and its risk factor to human health has increased. At present, there are four commonly used mycomycin detection methods in the laboratory. Simultaneous detection of multiple mycomycins is mainly to directly extract the mycotoxins in the sample and use liquid chromatography-mass spectrometry for simultaneous determination. However, this method needs to add Full-carbon-labeled stable isotope mycin is used as an internal standard, but it is expensive and the experimental cost is high. Now there is a need for an extraction method that can shorten the extraction time, improve detection efficiency, reduce costs, and improve safety. Con...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01D11/04
CPCB01D11/0492
Inventor 刘承兰邓年
Owner SOUTH CHINA AGRI UNIV
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