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Compound enzyme for eggshell membrane hydrolysis, eggshell membrane hydrolysis method and eggshell membrane hydrolysis product and extracting method thereof

A hydrolyzate and extraction method technology, applied in the field of bioengineering, can solve the problems of insufficient enzymatic hydrolysis of eggshell membranes, low yield of eggshell membrane hydrolyzate extraction, insufficient enzymatic hydrolysis, etc., and achieve reasonable temperature and pH values , reasonable settings, and the effect of improving the yield

Active Publication Date: 2018-11-13
四川德博尔制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The invention provides a compound enzyme for hydrolyzing eggshell membranes, aiming to improve the problem of insufficient hydrolysis of eggshell membranes by existing enzymes
[0005] The invention provides a method for hydrolyzing eggshell membranes, aiming at improving the problem of insufficient enzymatic hydrolysis of existing eggshell membranes
[0006] The present invention also provides a method for extracting eggshell membrane hydrolyzate, aiming at improving the problem of low yield of extracting eggshell membrane hydrolyzate in the existing enzymatic hydrolysis method

Method used

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  • Compound enzyme for eggshell membrane hydrolysis, eggshell membrane hydrolysis method and eggshell membrane hydrolysis product and extracting method thereof
  • Compound enzyme for eggshell membrane hydrolysis, eggshell membrane hydrolysis method and eggshell membrane hydrolysis product and extracting method thereof
  • Compound enzyme for eggshell membrane hydrolysis, eggshell membrane hydrolysis method and eggshell membrane hydrolysis product and extracting method thereof

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Embodiment 1

[0043] This embodiment provides a compound enzyme for hydrolyzing eggshell membranes, a method for hydrolyzing eggshell membranes, a hydrolyzate of eggshell membranes and an extraction method thereof.

[0044] A composite enzyme for hydrolyzing eggshell membranes, comprising keratinase and alkaline protease at a mass ratio of 0.2:1.

[0045] A method for hydrolyzing eggshell membranes, comprising: drying clean eggshell membranes and pulverizing them into eggshell membrane dry powder, mixing the eggshell membrane dry powder with water 8 times its weight, keeping the temperature at 70°C for 240 minutes, and then cooling to 50°C to obtain Eggshell membrane fluid. The eggshell liquid membrane is kept at 50°C with a mass concentration of 1M hydrochloric acid solution to adjust the pH to 8.0, and compound enzymes are added therein, wherein the amount of keratinase added is 0.2% of the weight of the eggshell membrane dry powder, and the addition of alkaline protease The amount is 1%...

Embodiment 2

[0048] This embodiment provides a compound enzyme for hydrolyzing eggshell membranes, a method for hydrolyzing eggshell membranes, a hydrolyzate of eggshell membranes and an extraction method thereof.

[0049] A composite enzyme for hydrolyzing eggshell membranes, comprising keratinase and alkaline protease at a mass ratio of 5:1.

[0050] A method for hydrolyzing eggshell membranes, comprising: drying clean eggshell membranes and pulverizing them into eggshell membrane dry powder, mixing the eggshell membrane dry powder with water 20 times its weight, keeping the temperature at 80°C for 10 minutes, and then cooling to 55°C to obtain Eggshell membrane fluid. The eggshell liquid membrane is kept at 55°C with a mass concentration of 4M hydrochloric acid solution to adjust the pH to 8.5, and compound enzymes are added therein, wherein the amount of keratinase added is 1% of the weight of the eggshell membrane dry powder, and the addition of alkaline protease The amount is 0.2% o...

Embodiment 3

[0053] This embodiment provides a compound enzyme for hydrolyzing eggshell membranes, a method for hydrolyzing eggshell membranes, a hydrolyzate of eggshell membranes and an extraction method thereof.

[0054] A composite enzyme for hydrolyzing eggshell membranes, comprising keratinase and alkaline protease at a mass ratio of 1:1.

[0055] A method for hydrolyzing eggshell membranes, comprising: drying clean eggshell membranes and pulverizing them into eggshell membrane dry powder, mixing the eggshell membrane dry powder with water 15 times its weight, keeping the temperature at 75°C for 30 minutes, and then cooling to 52°C to obtain Eggshell membrane fluid. The eggshell liquid membrane is kept at 52°C with a mass concentration of 3M hydrochloric acid solution to adjust the pH to 8.2, and compound enzymes are added thereto, wherein the amount of keratinase added is 1% of the weight of the eggshell membrane dry powder, and the addition of alkaline protease The amount is 1% of ...

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Abstract

The invention relates to the technical field of bioengineering and provides a compound enzyme for eggshell membrane hydrolysis. The compound enzyme comprises keratinase and alkaline protease in a massratio of 0.2-5:1, and the compound enzyme enables complete hydrolysis of eggshell membranes. An eggshell membrane hydrolysis method includes: mixing eggshell membrane dried powder with water, keepingthe temperature at 70-80 DEG C for 10-240min, cooling to 50-55 DEG C, and keeping the temperature to obtain eggshell membrane liquid; adjusting pH of the eggshell membrane liquid in a range of 8.0-8.5, adding the compound enzyme, and continuing heat preservation to obtain eggshell membrane hydrolysis mixed liquid. By adoption of the hydrolysis method, complete hydrolysis of the eggshell membranescan be realized. An extracting method of an eggshell membrane hydrolysis product includes the eggshell membrane hydrolysis method and includes steps of adjusting pH to 5.0-8.0, heating to 90-100DEG C, keeping the temperature for 10-60min, filtering, concentrating and spray drying. The hydrolysis product obtained by the extracting method is high in yield and quality. The invention further providesthe eggshell membrane hydrolysis product prepared by adoption of the extracting method.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a compound enzyme for hydrolyzing eggshell membranes, a method for hydrolyzing eggshell membranes, a hydrolyzate of eggshell membranes and an extraction method thereof. Background technique [0002] The main component of eggshell membrane is protein, which exists in the form of glycoprotein, accounting for 90% of the total weight of the membrane, and has high medicinal value, but it is difficult to achieve a good medicinal effect by direct consumption. Extraction of eggshell membranes becomes the key. However, the yields of active substances such as glycoproteins in eggshell membranes extracted by the existing enzymatic hydrolysis methods are all low. [0003] In view of this, the present application is proposed. Contents of the invention [0004] The invention provides a compound enzyme for hydrolyzing eggshell membranes, aiming at improving the problem of insufficien...

Claims

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Application Information

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IPC IPC(8): C12N9/50C12P21/06A61K35/57
CPCA61K35/57C12N9/50C12P21/06
Inventor 张革唐章勇陈诚陈云
Owner 四川德博尔制药有限公司