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A kind of method for detecting rice flax leaf spot pathogen

A technology of flax leaf spot fungus and flax leaf spot disease is applied in the field of molecular biology to achieve high specificity and improve identification efficiency

Active Publication Date: 2022-04-19
YIELEAD RICE IND
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide an effective method for quickly identifying rice flax leaf spot strains based on DNA molecular marker technology in view of the current problems in the identification of rice flax leaf spot pathogens

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  • A kind of method for detecting rice flax leaf spot pathogen

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Embodiment 1

[0032] The following methods were used to identify rice leaf samples of flax leaf spot disease obtained in the field:

[0033] (1) After culturing the preserved known flax leaf spot pathogen on potato agar sucrose medium for 10 days, cut 3 pieces of mycelia (5mm×5mm size) along the edge of the colony and transfer them to 100mL potato sucrose liquid medium In a conical flask, culture at 27°C on a shaking table at 140rpm for 8 days, filter directly with gauze, rinse with sterile water 2 to 3 times, collect the mycelial balls, absorb the moisture on the surface of the mycelium with filter paper, and store in a -20°C refrigerator spare;

[0034] (2) After the mycelium was freeze-dried, the CTAB method (refer to "Molecular Cloning Experiment Guide") was used to extract DNA, and the concentration and purity of the extracted DNA were detected by a UV spectrophotometer, and ddH 2 O After adjusting the DNA concentration to 100ng / μL, put the detected DNA sample in a -20°C refrigerator ...

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Abstract

The invention discloses a method for detecting rice flax leaf spot pathogen, and relates to the field of molecular biology. The invention transforms SCAR markers with RAPD primers, can quickly identify the pathogen of rice flax leaf spot disease, and has high specificity, and does not need to design and screen specific primers according to the genome DNA sequence of rice flax leaf spot disease bacteria, and can directly extract the sample DNA sequence. It can effectively improve the identification efficiency of rice flax leaf spot pathogen.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to a method for detecting rice flax leaf spot pathogen. Background technique [0002] Rice flax leaf spot disease, also known as rice flax spot disease, flax leaf blight. All can be ill from the seedling stage to the harvest stage, and the aboveground part of the rice plant can be damaged, especially the leaves are the most common. The disease occurs in the bud stage, the cotyledons turn brown, the buds are not drawn out, and the cotyledons die. The leaves and leaf sheaths at the seedling stage are mostly oval scabs, the size of flax grains, dark brown, and sometimes the scabs expand and connect into strips, and the seedlings die when there are many scabs. The leaves of adult plants are infected, initially as small brown spots, which gradually expand into elliptical spots, the size of sesame seeds, the center of the lesion is grayish brown to grayish white, the edges are brown, an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12Q1/6848C12Q1/04
CPCC12Q1/6848C12Q1/6895C12Q2600/156C12Q2549/119
Inventor 乔保建乔琪任代胜夏祥华付锡江陶元平彭冲
Owner YIELEAD RICE IND