Detection card for detecting trichlorohydroxydiphenyl ether, and preparation method and application thereof
A technology of trichlorohydroxydiphenyl ether and a detection card, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of high detection cost, solvent consumption, cumbersome processing, etc., achieve strong specificity, wide application range, and avoid The effect of false detection problems
- Application Information
AI Technical Summary
Problems solved by technology
 A test card for detecting triclosan, such as figure 1 As shown, the test card is provided with a sample pad 1, a gold standard pad 2, a nitrocellulose membrane 3 and a water-absorbing pad 7 which are sequentially lapped and pasted on the backing; A first quality control line 4, a detection line 6 and a second quality control line 5 are arranged in turn from the end to the end of the absorbent pad;
 The first antibody labeled with colloidal gold is coated on the gold label pad 2, and the first antibody is an antibody specific for anti-triclosan; the detection line 6 is coated with triclosan complete antigen, the complete antigen is a conjugate of triclosan and carrier protein; the first quality control line 4 and the second quality control line 5 are coated with a second antibody, in this embodiment , the second antibody is a commercially available goat anti-mouse IgG polyclonal antibody, which can specifically bind to the first antibody and does not bind to the...
 The detection card preparation method of the detection trichlorohydroxy diphenyl ether of embodiment 1 comprises the following steps:
 (1) Synthesis of triclosan-carrier protein conjugates:
 Disperse 0.29g of trichlorohydroxydiphenyl ether and 1.38g of potassium carbonate into 50mL of pyridine, stir for 30min, add 0.39g of ethyl 4-bromobutyrate, and heat to reflux for 6h, so that the phenolic hydroxyl group of trichlorohydroxydiphenyl ether is covered with butyric acid Ethyl substitution, to obtain an intermediate product, then add excess trifluoroacetic acid, reflux and stir for 1h, remove the ethyl group, expose the carboxyl group, and obtain the trichlorohydroxydiphenyl ether hapten. The purified triclosan hapten, N-hydroxysuccinimide and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide were mixed in pyridine and stirred overnight.
 Weigh 0.144g of bovine serum carrier protein (commercially available), dissolve in 5mL of 0.1mol / L NaHCO at pH 7.0...
 The detection card of Example 1 was used to carry out the detection limit test.
 1. Method.
 Accurately weigh trichlorohydroxydiphenyl ether, dissolve it in a small amount of methanol, and then use 50% methanol solution as a diluent to prepare a standard working solution with concentrations of 0ng / mL, 5ng / mL, 10ng / mL, and 20ng / mL , 50ng / mL.
 Take the above-mentioned standard working solution as a sample and add it dropwise to the test card for detecting trichlorohydroxydiphenyl ether in Example 1, and observe the result 5 minutes after adding the sample.
 2. Results.
 The color development results of the detection line T and the quality control line C are shown in Table 1.
 Table 1 Trichlorohydroxydiphenyl ether minimum detection limit test
 It can be seen from the above results that the test card for the semi-quantitative detection of triclosan in Example 1 above can detect all three tr...
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction