Detection card for detecting trichlorohydroxydiphenyl ether, and preparation method and application thereof

A technology of trichlorohydroxydiphenyl ether and a detection card, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of high detection cost, solvent consumption, cumbersome processing, etc., achieve strong specificity, wide application range, and avoid The effect of false detection problems

Inactive Publication Date: 2018-11-13
GUANGZHOU QUALITY SUPERVISION & TESTING INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chromatography and other instrumental methods have the advantages of high specificity and accurate results, but the disadvantages are that the sample pretreatment is cumbersome, consumes a large amount of solvent, and requires expensive laboratory equipment, and the detection cost is high

Method used

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  • Detection card for detecting trichlorohydroxydiphenyl ether, and preparation method and application thereof
  • Detection card for detecting trichlorohydroxydiphenyl ether, and preparation method and application thereof
  • Detection card for detecting trichlorohydroxydiphenyl ether, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] A test card for detecting triclosan, such as figure 1 As shown, the test card is provided with a sample pad 1, a gold standard pad 2, a nitrocellulose membrane 3 and a water-absorbing pad 7 which are sequentially lapped and pasted on the backing; A first quality control line 4, a detection line 6 and a second quality control line 5 are arranged in turn from the end to the end of the absorbent pad;

[0055] The first antibody labeled with colloidal gold is coated on the gold label pad 2, and the first antibody is an antibody specific for anti-triclosan; the detection line 6 is coated with triclosan complete antigen, the complete antigen is a conjugate of triclosan and carrier protein; the first quality control line 4 and the second quality control line 5 are coated with a second antibody, in this embodiment , the second antibody is a commercially available goat anti-mouse IgG polyclonal antibody, which can specifically bind to the first antibody and does not bind to the...

Embodiment 2

[0067] The detection card preparation method of the detection trichlorohydroxy diphenyl ether of embodiment 1 comprises the following steps:

[0068] (1) Synthesis of triclosan-carrier protein conjugates:

[0069] Disperse 0.29g of trichlorohydroxydiphenyl ether and 1.38g of potassium carbonate into 50mL of pyridine, stir for 30min, add 0.39g of ethyl 4-bromobutyrate, and heat to reflux for 6h, so that the phenolic hydroxyl group of trichlorohydroxydiphenyl ether is covered with butyric acid Ethyl substitution, to obtain an intermediate product, then add excess trifluoroacetic acid, reflux and stir for 1h, remove the ethyl group, expose the carboxyl group, and obtain the trichlorohydroxydiphenyl ether hapten. The purified triclosan hapten, N-hydroxysuccinimide and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide were mixed in pyridine and stirred overnight.

[0070] Weigh 0.144g of bovine serum carrier protein (commercially available), dissolve in 5mL of 0.1mol / L NaHCO at pH 7.0...

Embodiment 3

[0083] The detection card of Example 1 was used to carry out the detection limit test.

[0084] 1. Method.

[0085] Accurately weigh trichlorohydroxydiphenyl ether, dissolve it in a small amount of methanol, and then use 50% methanol solution as a diluent to prepare a standard working solution with concentrations of 0ng / mL, 5ng / mL, 10ng / mL, and 20ng / mL , 50ng / mL.

[0086] Take the above-mentioned standard working solution as a sample and add it dropwise to the test card for detecting trichlorohydroxydiphenyl ether in Example 1, and observe the result 5 minutes after adding the sample.

[0087] 2. Results.

[0088] The color development results of the detection line T and the quality control line C are shown in Table 1.

[0089] Table 1 Trichlorohydroxydiphenyl ether minimum detection limit test

[0090]

[0091]

[0092] It can be seen from the above results that the test card for the semi-quantitative detection of triclosan in Example 1 above can detect all three tr...

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Abstract

The invention relates to a detection card for detecting trichlorohydroxydiphenyl ether, and a preparation method and an application thereof, and belongs to the technical field of additive detection. The detection card is equipped with a sample pad, a gold-labelled pad, a nitrate cellulose membrane and a water absorbent pad which are overlapped and pasted on a backing sequentially; a first qualitycontrol line, a detection line and a second quality control line are arranged on the nitrate cellulose membrane from a sample pad end to a water absorbent pad end sequentially; the gold-labelled pad is coated with a first antibody labeled with colloidal gold, and the first antibody is special trichlorohydroxydiphenyl ether-resisting antibody; the detection line is coated with a complete antigen, and the complete antigen is a conjugate of trichlorohydroxydiphenyl ether and a carrier protein; the first quality control line and the second quality control line are each coated with a second antibody, and the second antibody can be specifically combined with the first antibody and is not combined with the complete antigen. The detection card adopts double quality control lines, has the advantages of accurate results, simple structure and convenient use, and is suitable for rapid detection of trichlorohydroxydiphenyl ether.

Description

technical field [0001] The invention relates to the technical field of detection devices for food, food packaging and daily chemical products, in particular to a detection card for detecting trichlorohydroxydiphenyl ether, its preparation method and application. Background technique [0002] Triclosan, also known as triclosan, is a broad-spectrum high-efficiency antibacterial agent that can kill and inhibit Gram-positive and negative bacteria, fungi, viruses, etc., and is widely used in agricultural production, In the fields of food processing, food packaging materials and daily chemical products, its structure is shown in the following formula I. [0003] [0004] Food packaging materials added with trichlorohydroxydiphenyl ether have certain antibacterial properties, which can inhibit the growth of microorganisms and prolong the shelf life while ensuring food quality, safety and freshness. Food packaging products added with TCH mainly include: cooked food packaging box...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/558G01N33/543G01N33/531
CPCG01N33/531G01N33/543G01N33/558G01N33/577
Inventor 陈立伟黄金凤吴楚生寻知庆黄宇峰郭燕华郭新东侯向昶吴玉銮冼燕萍
Owner GUANGZHOU QUALITY SUPERVISION & TESTING INST
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