Test paper strip for detecting morphine as well as preparation method and application method thereof

A technology of test strips and morphine, applied in the field of morphine detection, can solve the problems of inability to realize rapid detection of large batches of samples, time-consuming, complicated operation, etc., and achieve the effects of short detection time, simple storage and simple operation

Inactive Publication Date: 2018-11-13
郑州左安检测科技有限公司
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  • Abstract
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  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to overcome the existing methods for detecting morphine, which are complex in operation, time-consuming, and unable to realize rapid detection of large batches of samples, and provide a method with simple operation, high sensitivity, fast detection speed, Low-cost test strip for detecting morphine and its preparation method and application method, so as to realize rapid detection and on-site monitoring of large quantities of morphine samples

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  • Test paper strip for detecting morphine as well as preparation method and application method thereof
  • Test paper strip for detecting morphine as well as preparation method and application method thereof
  • Test paper strip for detecting morphine as well as preparation method and application method thereof

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preparation example Construction

[0041] The preparation method of the test strip for detecting morphine mainly comprises the following steps:

[0042] 1) Label the morphine antibody with fluorescent microspheres and spray it on the conjugate release pad to prepare the conjugate release pad sprayed with fluorescent microsphere-labeled morphine antibody;

[0043] 2) Spray the hapten-carrier protein conjugate and goat anti-mouse secondary antibody on the reaction membrane as the test line (T) and quality control line (C) to prepare the morphine-coated hapten-carrier protein conjugate The detection line of the substance and the reaction membrane of the quality control line coated with goat anti-mouse secondary antibody;

[0044] 3) Assemble the conjugate release pad prepared in 1) and 2), the reaction membrane, the sample absorption pad, the water absorption pad and the bottom plate into a test strip.

[0045] The following step-by-step detailed description:

[0046] 1. Preparation of fluorescent microsphere ma...

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Abstract

The invention discloses a test paper strip for detecting morphine as well as a preparation method and an application method thereof. The test paper strip comprises a sample absorption pad, a conjugaterelease pad, a reaction membrane, a water absorbing pad and a substrate in sequence, wherein the conjugate release pad is coated with an antibody marked by time resolved fluorescence microspheres; the reaction membrane is coated with a detection line of hapten-carrier protein conjugate and a quality control line of a goat-anti-mouse second antibody. Due to adoption of the time resolved fluorescence marked antibody and an immunochromatography technique, rapid immunoassay of morphine can be achieved. The invention further provides a method for detecting morphine in a sample by using the test paper strip. The test paper strip has the advantages of being high in sensitivity, accurate in quantification, rapid in detection, convenient to operate, economical, and practical, and rapid detection and on-site detection on large scales of morphine samples can be achieved.

Description

technical field [0001] The invention relates to the detection of morphine, in particular to a test strip for quantitative detection of morphine by using time-resolved fluorescent microsphere immunochromatography technology and its preparation method and application method. Background technique [0002] Morphine is the main component of opium and heroin. It is extracted from opium and is a pure opioid receptor agonist with analgesic and hypnotic effects. Therefore, in medicine, morphine is a narcotic analgesic. Morphine has the function of depressing the central nervous system, and eaters are prone to develop tolerance and dependence, and are easily addicted and abused. [0003] The current detection and monitoring methods for morphine mainly include high performance liquid chromatography, gas chromatography, liquid chromatography or gas chromatography, etc. These chromatographic methods have good sensitivity and specificity, but the operation is complicated, the throughput ...

Claims

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Application Information

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IPC IPC(8): G01N33/94
CPCG01N33/9486
Inventor 吕小翠张振兴贾嘉赵琳周亮崔浩哲张鹏高翔
Owner 郑州左安检测科技有限公司
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